Values CatLog read from this paper
A highly active adipyl-cephalosporin acylase obtained via rational randomization
PMID 17922842doi:10.1111/j.1742-4658.2007.06081.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 17 records (34 values) from this paper: glutaryl acylase (cephalosporin acylase) SY-77 from Pseudomonas.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 17 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glutaryl acylase (cephalosporin acylase) SY-77 | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 0.38 | 0.8 | 0.47 calc. | Open record: glutaryl acylase (cephalosporin acylase) SY-77, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77 | Pseudomonas | glutaryl-7-aminocephalosporanic acid | 4.1 | 0.042 | 98 calc. | Open record: glutaryl acylase (cephalosporin acylase) SY-77, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant N266Q/F375L | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 0.48 | 0.17 | 2.8 calc. | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant N266Q/F375L, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant N266Q/F375L | Pseudomonas | glutaryl-7-aminocephalosporanic acid | 0.75 | 0.17 | 4.4 calc. | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant N266Q/F375L, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 0.66 | 0.47 | 1.4 calc. | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H | Pseudomonas | glutaryl-7-aminocephalosporanic acid | 2.5 | 0.08 | 31 calc. | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/F229L | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 0.71 | 1.1 | 0.65 calc. | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/F229L, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/F375H | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 0.44 | 0.56 | 0.79 calc. | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/F375H, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/F375L | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 1 | 0.7 | 1.4 calc. | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/F375L, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/M271V/Q291K/T374S | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 0.44 | 0.8 | 0.55 calc. | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/M271V/Q291K/T374S, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/N266H | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 0.95 | 0.19 | 5.0 calc. | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/N266H, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/N266H | Pseudomonas | glutaryl-7-aminocephalosporanic acid | 2.2 | 0.08 | 28 calc. | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/N266H, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/N266M | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 1.2 | 0.17 | 7.1 calc. | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/N266M, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/N266M | Pseudomonas | glutaryl-7-aminocephalosporanic acid | 0.89 | 0.3 | 3.0 calc. | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/N266M, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/N266Q/F375M | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 0.72 | 0.28 | 2.6 calc. | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/N266Q/F375M, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/N266Q/F375M | Pseudomonas | glutaryl-7-aminocephalosporanic acid | 0.72 | 0.62 | 1.2 calc. | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/N266Q/F375M, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylase (cephalosporin acylase) SY-77variant Y178H/N266S | Pseudomonas | adipyl-7-aminodesacetoxycephalosporanic acid | 0.43 | 0.9 | 0.48 calc. | Open record: glutaryl acylase (cephalosporin acylase) SY-77 variant Y178H/N266S, adipyl-7-aminodesacetoxycephalosporanic acid |