Values CatLog read from this paper
Mutational analysis of a key residue in the substrate specificity of a cephalosporin acylase
PMID 15174165doi:10.1002/cbic.200300764Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 31 records (62 values) from this paper: glutaryl acylase from Pseudomonas sp..
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 31 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glutaryl acylase | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.41 | 1.2 | 0.34 calc. | Open record: glutaryl acylase, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylase | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 4 | 0.031 | 130 calc. | Open record: glutaryl acylase, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266A | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.18 | 2.2 | 0.082 calc. | Open record: glutaryl acylase variant N266A, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266A | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 1.4 | 0.26 | 5.4 calc. | Open record: glutaryl acylase variant N266A, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266C | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.31 | 0.8 | 0.39 calc. | Open record: glutaryl acylase variant N266C, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266C | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 2 | 0.16 | 12 calc. | Open record: glutaryl acylase variant N266C, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266D | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.002 | 2.2 | 9.1 × 10⁻⁴ calc. | Open record: glutaryl acylase variant N266D, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266E | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.023 | 13.8 | 0.0017 calc. | Open record: glutaryl acylase variant N266E, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266E | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 0.025 | 0.29 | 0.086 calc. | Open record: glutaryl acylase variant N266E, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266F | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.37 | 0.6 | 0.62 calc. | Open record: glutaryl acylase variant N266F, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266F | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 0.9 | 0.1 | 9.0 calc. | Open record: glutaryl acylase variant N266F, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266G | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.23 | 1.4 | 0.16 calc. | Open record: glutaryl acylase variant N266G, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266G | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 2 | 0.54 | 3.7 calc. | Open record: glutaryl acylase variant N266G, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266H | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.47 | 0.14 | 3.4 calc. | Open record: glutaryl acylase variant N266H, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266H | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 3.1 | 0.044 | 70 calc. | Open record: glutaryl acylase variant N266H, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266L | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.25 | 1 | 0.25 calc. | Open record: glutaryl acylase variant N266L, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266L | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 0.082 | 0.43 | 0.19 calc. | Open record: glutaryl acylase variant N266L, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266M | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 1.9 | 0.33 | 5.8 calc. | Open record: glutaryl acylase variant N266M, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266M | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 1.8 | 0.17 | 11 calc. | Open record: glutaryl acylase variant N266M, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266P | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.061 | 4.4 | 0.014 calc. | Open record: glutaryl acylase variant N266P, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266P | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 0.13 | 1.3 | 0.10 calc. | Open record: glutaryl acylase variant N266P, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266Q | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.46 | 0.62 | 0.74 calc. | Open record: glutaryl acylase variant N266Q, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266Q | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 2.4 | 0.12 | 20 calc. | Open record: glutaryl acylase variant N266Q, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266S | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.24 | 0.99 | 0.24 calc. | Open record: glutaryl acylase variant N266S, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266S | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 1.6 | 0.075 | 21 calc. | Open record: glutaryl acylase variant N266S, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266T | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.15 | 5.7 | 0.026 calc. | Open record: glutaryl acylase variant N266T, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266T | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 0.49 | 1.5 | 0.33 calc. | Open record: glutaryl acylase variant N266T, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266W | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.33 | 0.59 | 0.56 calc. | Open record: glutaryl acylase variant N266W, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266W | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 1.3 | 0.18 | 7.2 calc. | Open record: glutaryl acylase variant N266W, glutaryl-7-aminocephalosporanic acid | |
| glutaryl acylasevariant N266Y | Pseudomonas sp. | adipyl-7-aminodesacetoxycephalosporanic acid | 0.33 | 0.65 | 0.51 calc. | Open record: glutaryl acylase variant N266Y, adipyl-7-aminodesacetoxycephalosporanic acid | |
| glutaryl acylasevariant N266Y | Pseudomonas sp. | glutaryl-7-aminocephalosporanic acid | 0.61 | 0.11 | 5.5 calc. | Open record: glutaryl acylase variant N266Y, glutaryl-7-aminocephalosporanic acid |