Values CatLog read from this paper
Crucial role of conserved lysine 277 in the fidelity of tRNA aminoacylation by Escherichia coli valyl-tRNA synthetase
PMID 12475234doi:10.1021/bi0205101Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (17 values) from this paper: valyl-tRNA synthetase (ValRS) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| valyl-tRNA synthetase (ValRS) | Escherichia coli | ATP | — | 0.11 | — | pH 7.5, 25 °C | Open record: valyl-tRNA synthetase (ValRS), ATP |
| valyl-tRNA synthetase (ValRS) | Escherichia coli | L-valine[32P]PPi-ATP exchange (initial rates); 2 mM valine; 2 mM ATP | 51 | — | — | pH 7.5, 25 °C | Open record: valyl-tRNA synthetase (ValRS), L-valine |
| valyl-tRNA synthetase (ValRS) | Escherichia coli | L-valinevaline-dependent [32P]PPi-ATP exchange (initial rates) | 50 | 0.07 | 710 calc. | pH 7.5, 25 °C | Open record: valyl-tRNA synthetase (ValRS), L-valine |
| valyl-tRNA synthetase (ValRS) | Escherichia coli | L-valinetRNAVal aminoacylation (initial rates); 2 mM ATP | 1.5 | 0.047 | 32 calc. | pH 7.5, 25 °C | Open record: valyl-tRNA synthetase (ValRS), L-valine |
| valyl-tRNA synthetase (ValRS) | Escherichia coli | tRNAVal17.7 µM [14C]valine; 7 mM MgCl2; various tRNAVal1 | 1.7 | 8 × 10⁻⁵ | 2.1 × 10⁴ calc. | pH 7.5, 25 °C | Open record: valyl-tRNA synthetase (ValRS), tRNAVal1 |
| valyl-tRNA synthetase (ValRS) | Escherichia coli | tRNAVal17 mM MgCl2 (5 mM free Mg2+ excess); His6-tagged | — | 1 × 10⁻⁴ | — | pH 7.5, 25 °C | Open record: valyl-tRNA synthetase (ValRS), tRNAVal1 |
| valyl-tRNA synthetase (ValRS)variant K277A | Escherichia coli | ATP | — | 0.11 | — | pH 7.5, 25 °C | Open record: valyl-tRNA synthetase (ValRS) variant K277A, ATP |
| valyl-tRNA synthetase (ValRS)variant K277A | Escherichia coli | L-valinevaline-dependent [32P]PPi-ATP exchange (initial rates) | 57 | 0.072 | 790 calc. | pH 7.5, 25 °C | Open record: valyl-tRNA synthetase (ValRS) variant K277A, L-valine |
| valyl-tRNA synthetase (ValRS)variant K277A | Escherichia coli | L-valinetRNAVal aminoacylation (initial rates); 20 mM Tris-HCl | 0.064 | 0.0019 | 34 calc. | pH 7.5, 25 °C | Open record: valyl-tRNA synthetase (ValRS) variant K277A, L-valine |
| valyl-tRNA synthetase (ValRS)variant K277A | Escherichia coli | L-valinetRNAVal aminoacylation; cosubstrate tRNAVal1; ATP (2 mM) | 1.7 | 0.011 | 150 calc. | pH 7.5, 25 °C | Open record: valyl-tRNA synthetase (ValRS) variant K277A, L-valine |
| valyl-tRNA synthetase (ValRS)variant K277A | Escherichia coli | tRNAVal1 | — | 2.6 × 10⁻⁴ | — | pH 7.5, 25 °C | Open record: valyl-tRNA synthetase (ValRS) variant K277A, tRNAVal1 |