CatLogenzyme kinetics catalog

EC 6.1.1.9: valine-tRNA ligase

LigasesOpen in Browse

CatLog read 26 records for EC 6.1.1.9 from 2 papers, for 2 organisms. Names in these papers: valyl-tRNA synthetase (ValRS).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 31 records for EC 6.1.1.9 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.1.1.9 in Browse.

Papers

PaperOrganismsRecords
Mutational analysis suggests the same design for editing activities of two tRNA synthetasesBacillus stearothermophilus15
Crucial role of conserved lysine 277 in the fidelity of tRNA aminoacylation by Escherichia coli valyl-tRNA synthetaseEscherichia coli11

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
valyl-tRNA synthetase (ValRS)Bacillus stearothermophilusThr-tRNAVal1.47——pH 7.5, 25 °CPMID 8611551Open
valyl-tRNA synthetase (ValRS)Bacillus stearothermophilusthreonine12.8160.8pH 7.5, 37 °CPMID 8611551Open
valyl-tRNA synthetase (ValRS)Bacillus stearothermophilusvaline18.10.059310pH 7.5, 37 °CPMID 8611551Open
valyl-tRNA synthetase (ValRS)variant D87ABacillus stearothermophilusThr-tRNAVal1.36——pH 7.5, 25 °CPMID 8611551Open
valyl-tRNA synthetase (ValRS)variant D87ABacillus stearothermophilusthreonine0.019——pH 7.5, 37 °CPMID 8611551Open
valyl-tRNA synthetase (ValRS)variant D87ABacillus stearothermophilusvaline0.0676.40.011pH 7.5, 37 °CPMID 8611551Open
valyl-tRNA synthetase (ValRS)variant G85RBacillus stearothermophilusthreonine0.173260.0067pH 7.5, 37 °CPMID 8611551Open
valyl-tRNA synthetase (ValRS)variant G85RBacillus stearothermophilusvaline0.920.1864.9pH 7.5, 37 °CPMID 8611551Open
valyl-tRNA synthetase (ValRS)variant P47ABacillus stearothermophilusthreonine8.43190.44pH 7.5, 37 °CPMID 8611551Open
valyl-tRNA synthetase (ValRS)variant P47ABacillus stearothermophilusvaline26.70.189140pH 7.5, 37 °CPMID 8611551Open
valyl-tRNA synthetase (ValRS)variant P47GBacillus stearothermophilusthreonine0.583380.015pH 7.5, 37 °CPMID 8611551Open
valyl-tRNA synthetase (ValRS)variant P47GBacillus stearothermophilusvaline6.450.1446pH 7.5, 37 °CPMID 8611551Open
valyl-tRNA synthetase (ValRS)variant P47IBacillus stearothermophilusThr-tRNAVal1.25——pH 7.5, 25 °CPMID 8611551Open
valyl-tRNA synthetase (ValRS)variant P47IBacillus stearothermophilusthreonine0.027——pH 7.5, 37 °CPMID 8611551Open
valyl-tRNA synthetase (ValRS)variant P47IBacillus stearothermophilusvaline0.0241102.2 × 10⁻⁴ calc.pH 7.5, 37 °CPMID 8611551Open
valyl-tRNA synthetase (ValRS)Escherichia coliATP—0.11—pH 7.5, 25 °CPMID 12475234Open
valyl-tRNA synthetase (ValRS)Escherichia coliL-valine[32P]PPi-ATP exchange (initial rates); 2 mM valine; 2 mM ATP51——pH 7.5, 25 °CPMID 12475234Open
valyl-tRNA synthetase (ValRS)Escherichia coliL-valinevaline-dependent [32P]PPi-ATP exchange (initial rates)500.07710 calc.pH 7.5, 25 °CPMID 12475234Open
valyl-tRNA synthetase (ValRS)Escherichia coliL-valinetRNAVal aminoacylation (initial rates); 2 mM ATP1.50.04732 calc.pH 7.5, 25 °CPMID 12475234Open
valyl-tRNA synthetase (ValRS)Escherichia colitRNAVal17.7 µM [14C]valine; 7 mM MgCl2; various tRNAVal11.78 × 10⁻⁵2.1 × 10⁴ calc.pH 7.5, 25 °CPMID 12475234Open
valyl-tRNA synthetase (ValRS)Escherichia colitRNAVal17 mM MgCl2 (5 mM free Mg2+ excess); His6-tagged—1 × 10⁻⁴—pH 7.5, 25 °CPMID 12475234Open
valyl-tRNA synthetase (ValRS)variant K277AEscherichia coliATP—0.11—pH 7.5, 25 °CPMID 12475234Open
valyl-tRNA synthetase (ValRS)variant K277AEscherichia coliL-valinevaline-dependent [32P]PPi-ATP exchange (initial rates)570.072790 calc.pH 7.5, 25 °CPMID 12475234Open
valyl-tRNA synthetase (ValRS)variant K277AEscherichia coliL-valinetRNAVal aminoacylation (initial rates); 20 mM Tris-HCl0.0640.001934 calc.pH 7.5, 25 °CPMID 12475234Open
valyl-tRNA synthetase (ValRS)variant K277AEscherichia coliL-valinetRNAVal aminoacylation; cosubstrate tRNAVal1; ATP (2 mM)1.70.011150 calc.pH 7.5, 25 °CPMID 12475234Open
valyl-tRNA synthetase (ValRS)variant K277AEscherichia colitRNAVal1—2.6 × 10⁻⁴—pH 7.5, 25 °CPMID 12475234Open