Values CatLog read from this paper
Mutational analysis suggests the same design for editing activities of two tRNA synthetases
PMID 8611551doi:10.1021/bi960011yOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 15 records (34 values) from this paper: valyl-tRNA synthetase (ValRS) from Bacillus stearothermophilus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| valyl-tRNA synthetase (ValRS) | Bacillus stearothermophilus | Thr-tRNAVal | 1.47 | — | — | pH 7.5, 25 °C | Open record: valyl-tRNA synthetase (ValRS), Thr-tRNAVal |
| valyl-tRNA synthetase (ValRS) | Bacillus stearothermophilus | threonine | 12.8 | 16 | 0.8 | pH 7.5, 37 °C | Open record: valyl-tRNA synthetase (ValRS), threonine |
| valyl-tRNA synthetase (ValRS) | Bacillus stearothermophilus | valine | 18.1 | 0.059 | 310 | pH 7.5, 37 °C | Open record: valyl-tRNA synthetase (ValRS), valine |
| valyl-tRNA synthetase (ValRS)variant D87A | Bacillus stearothermophilus | Thr-tRNAVal | 1.36 | — | — | pH 7.5, 25 °C | Open record: valyl-tRNA synthetase (ValRS) variant D87A, Thr-tRNAVal |
| valyl-tRNA synthetase (ValRS)variant D87A | Bacillus stearothermophilus | threonine | 0.019 | — | — | pH 7.5, 37 °C | Open record: valyl-tRNA synthetase (ValRS) variant D87A, threonine |
| valyl-tRNA synthetase (ValRS)variant D87A | Bacillus stearothermophilus | valine | 0.067 | 6.4 | 0.011 | pH 7.5, 37 °C | Open record: valyl-tRNA synthetase (ValRS) variant D87A, valine |
| valyl-tRNA synthetase (ValRS)variant G85R | Bacillus stearothermophilus | threonine | 0.173 | 26 | 0.0067 | pH 7.5, 37 °C | Open record: valyl-tRNA synthetase (ValRS) variant G85R, threonine |
| valyl-tRNA synthetase (ValRS)variant G85R | Bacillus stearothermophilus | valine | 0.92 | 0.186 | 4.9 | pH 7.5, 37 °C | Open record: valyl-tRNA synthetase (ValRS) variant G85R, valine |
| valyl-tRNA synthetase (ValRS)variant P47A | Bacillus stearothermophilus | threonine | 8.43 | 19 | 0.44 | pH 7.5, 37 °C | Open record: valyl-tRNA synthetase (ValRS) variant P47A, threonine |
| valyl-tRNA synthetase (ValRS)variant P47A | Bacillus stearothermophilus | valine | 26.7 | 0.189 | 140 | pH 7.5, 37 °C | Open record: valyl-tRNA synthetase (ValRS) variant P47A, valine |
| valyl-tRNA synthetase (ValRS)variant P47G | Bacillus stearothermophilus | threonine | 0.583 | 38 | 0.015 | pH 7.5, 37 °C | Open record: valyl-tRNA synthetase (ValRS) variant P47G, threonine |
| valyl-tRNA synthetase (ValRS)variant P47G | Bacillus stearothermophilus | valine | 6.45 | 0.14 | 46 | pH 7.5, 37 °C | Open record: valyl-tRNA synthetase (ValRS) variant P47G, valine |
| valyl-tRNA synthetase (ValRS)variant P47I | Bacillus stearothermophilus | Thr-tRNAVal | 1.25 | — | — | pH 7.5, 25 °C | Open record: valyl-tRNA synthetase (ValRS) variant P47I, Thr-tRNAVal |
| valyl-tRNA synthetase (ValRS)variant P47I | Bacillus stearothermophilus | threonine | 0.027 | — | — | pH 7.5, 37 °C | Open record: valyl-tRNA synthetase (ValRS) variant P47I, threonine |
| valyl-tRNA synthetase (ValRS)variant P47I | Bacillus stearothermophilus | valine | 0.024 | 110 | 2.2 × 10⁻⁴ calc. | pH 7.5, 37 °C | Open record: valyl-tRNA synthetase (ValRS) variant P47I, valine |