CatLogenzyme kinetics catalog

Values CatLog read from this paper

Analysis of the catalytic and binding residues of the diadenosine tetraphosphate pyrophosphohydrolase from Caenorhabditis elegans by site-directed mutagenesis

PMID 12475970doi:10.1074/jbc.m211983200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 17 records (34 values) from this paper: diadenosine tetraphosphate pyrophosphohydrolase (Ap4A hydrolase) from Caenorhabditis elegans.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 17 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.6.1.17

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
diadenosine tetraphosphate pyrophosphohydrolase (Ap4A hydrolase)Caenorhabditis elegansdiadenosine 5',5'''-P1,P4-tetraphosphate230.00882.6 × 10³ calc.Open
diadenosine tetraphosphate pyrophosphohydrolase (Ap4A hydrolase)variant E103QCaenorhabditis elegansdiadenosine 5',5'''-P1,P4-tetraphosphate0.750.0058130 calc.Open
diadenosine tetraphosphate pyrophosphohydrolase (Ap4A hydrolase)variant E52QCaenorhabditis elegansdiadenosine 5',5'''-P1,P4-tetraphosphate0.00520.00530.98 calc.Open
diadenosine tetraphosphate pyrophosphohydrolase (Ap4A hydrolase)variant E56QCaenorhabditis elegansdiadenosine 5',5'''-P1,P4-tetraphosphate2.4 × 10⁻⁴0.00790.030 calc.Open
diadenosine tetraphosphate pyrophosphohydrolase (Ap4A hydrolase)variant H31ACaenorhabditis elegansdiadenosine 5',5'''-P1,P4-tetraphosphate120.073160 calc.Open
diadenosine tetraphosphate pyrophosphohydrolase (Ap4A hydrolase)variant H31VCaenorhabditis elegansdiadenosine 5',5'''-P1,P4-tetraphosphate140.11130 calc.Open
diadenosine tetraphosphate pyrophosphohydrolase (Ap4A hydrolase)variant H38GCaenorhabditis elegansdiadenosine 5',5'''-P1,P4-tetraphosphate310.00585.3 × 10³ calc.Open
diadenosine tetraphosphate pyrophosphohydrolase (Ap4A hydrolase)variant H38KCaenorhabditis elegansdiadenosine 5',5'''-P1,P4-tetraphosphate310.00417.6 × 10³ calc.Open
diadenosine tetraphosphate pyrophosphohydrolase (Ap4A hydrolase)variant K36MCaenorhabditis elegansdiadenosine 5',5'''-P1,P4-tetraphosphate2.10.013160 calc.Open
diadenosine tetraphosphate pyrophosphohydrolase (Ap4A hydrolase)variant K79MCaenorhabditis elegansdiadenosine 5',5'''-P1,P4-tetraphosphate0.160.01511 calc.Open
diadenosine tetraphosphate pyrophosphohydrolase (Ap4A hydrolase)variant K81MCaenorhabditis elegansdiadenosine 5',5'''-P1,P4-tetraphosphate300.021.5 × 10³ calc.Open
diadenosine tetraphosphate pyrophosphohydrolase (Ap4A hydrolase)variant K83MCaenorhabditis elegansdiadenosine 5',5'''-P1,P4-tetraphosphate0.60.144.3 calc.Open
diadenosine tetraphosphate pyrophosphohydrolase (Ap4A hydrolase)variant Y121ACaenorhabditis elegansdiadenosine 5',5'''-P1,P4-tetraphosphate140.075190 calc.Open
diadenosine tetraphosphate pyrophosphohydrolase (Ap4A hydrolase)variant Y27ACaenorhabditis elegansdiadenosine 5',5'''-P1,P4-tetraphosphate260.0112.4 × 10³ calc.Open
diadenosine tetraphosphate pyrophosphohydrolase (Ap4A hydrolase)variant Y27DCaenorhabditis elegansdiadenosine 5',5'''-P1,P4-tetraphosphate290.0161.8 × 10³ calc.Open
diadenosine tetraphosphate pyrophosphohydrolase (Ap4A hydrolase)variant Y76ACaenorhabditis elegansdiadenosine 5',5'''-P1,P4-tetraphosphate20.05934 calc.Open
diadenosine tetraphosphate pyrophosphohydrolase (Ap4A hydrolase)variant Y76A/Y121ACaenorhabditis elegansdiadenosine 5',5'''-P1,P4-tetraphosphate1.10.03333 calc.Open