CatLogenzyme kinetics catalog

Values CatLog read from this paper

Molecular evolution by change of function. Alkaloid-specific homospermidine synthase retained all properties of deoxyhypusine synthase except binding the eIF5A precursor protein

PMID 12562768doi:10.1074/jbc.m207112200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 22 records (22 values) from this paper: deoxyhypusine synthase, homospermidine synthase from Senecio vernalis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.5.1.45, EC 2.5.1.46

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
deoxyhypusine synthaseSenecio vernaliseukaryotic initiation factor 5A precursor proteinstandard conditions: 0.1 M glycine-NaOH0.0022——pH 9.25, 30 °COpen
deoxyhypusine synthaseSenecio vernaliseukaryotic initiation factor 5A precursor protein[14C]spermidine fixed at 10 µM; 0.1 M glycine-NaOH—0.0013—pH 9.25, 30 °COpen
deoxyhypusine synthaseSenecio vernalisN-(3-aminopropyl)tetramethylene-1,4-diaminefixed putrescine 40 µM—0.005—pH 9.25, 30 °COpen
deoxyhypusine synthaseSenecio vernalisN-(3-aminopropyl)tetramethylene-1,4-diaminefixed putrescine 400 µM—0.027—pH 9.25, 30 °COpen
deoxyhypusine synthaseSenecio vernalisN-(3-aminopropyl)tetramethylene-1,4-diaminefixed putrescine 1000 µM—0.13—pH 9.25, 30 °COpen
deoxyhypusine synthaseSenecio vernalisN-(4-aminobutyl)tetramethylene-1,4-diaminefixed putrescine 40 µM—0.007—pH 9.25, 30 °COpen
deoxyhypusine synthaseSenecio vernalisN-(4-aminobutyl)tetramethylene-1,4-diaminefixed putrescine 400 µM—0.037—pH 9.25, 30 °COpen
deoxyhypusine synthaseSenecio vernalisN-(4-aminobutyl)tetramethylene-1,4-diaminefixed putrescine 1000 µM—0.054—pH 9.25, 30 °COpen
deoxyhypusine synthaseSenecio vernalisputrescine[14C]putrescine as labeled substrate; fixed spermidine 40 µM—0.31—pH 9.25, 30 °COpen
deoxyhypusine synthaseSenecio vernalisputrescine[14C]putrescine as labeled substrate; apparent Km; Table II—0.431—pH 9.25, 30 °COpen
deoxyhypusine synthaseSenecio vernalisputrescine[14C]putrescine as labeled substrate; apparent Km; Table II—1.057—pH 9.25, 30 °COpen
deoxyhypusine synthaseSenecio vernalisputrescine400 µM [14C]putrescine; 400 µM spermidine; kcat per subunit0.032——pH 9.25, 30 °COpen
homospermidine synthaseSenecio vernalisN-(3-aminopropyl)tetramethylene-1,4-diaminefixed putrescine 40 µM—0.014—pH 9.25, 30 °COpen
homospermidine synthaseSenecio vernalisN-(3-aminopropyl)tetramethylene-1,4-diaminefixed putrescine 400 µM—0.085—pH 9.25, 30 °COpen
homospermidine synthaseSenecio vernalisN-(3-aminopropyl)tetramethylene-1,4-diaminefixed putrescine 1000 µM—0.115—pH 9.25, 30 °COpen
homospermidine synthaseSenecio vernalisN-(4-aminobutyl)tetramethylene-1,4-diaminefixed putrescine 40 µM—0.007—pH 9.25, 30 °COpen
homospermidine synthaseSenecio vernalisN-(4-aminobutyl)tetramethylene-1,4-diaminefixed putrescine 400 µM—0.063—pH 9.25, 30 °COpen
homospermidine synthaseSenecio vernalisN-(4-aminobutyl)tetramethylene-1,4-diaminefixed putrescine 1000 µM—0.148—pH 9.25, 30 °COpen
homospermidine synthaseSenecio vernalisputrescine[14C]putrescine as labeled substrate; fixed spermidine 40 µM—0.137—pH 9.25, 30 °COpen
homospermidine synthaseSenecio vernalisputrescine[14C]putrescine as labeled substrate; apparent Km; Table II—0.176—pH 9.25, 30 °COpen
homospermidine synthaseSenecio vernalisputrescine[14C]putrescine as labeled substrate; apparent Km; Table II—0.196—pH 9.25, 30 °COpen
homospermidine synthaseSenecio vernalisputrescine400 µM [14C]putrescine; 400 µM spermidine; kcat per subunit0.032——pH 9.25, 30 °COpen