CatLogenzyme kinetics catalog

EC 2.5.1.46: deoxyhypusine synthase

TransferasesOpen in Browse

CatLog read 22 records for EC 2.5.1.46 from 2 papers, for 2 organisms. Names in these papers: deoxyhypusine synthase.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 16 records for EC 2.5.1.46 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.5.1.46 in Browse.

Papers

PaperOrganismsRecords
Molecular evolution by change of function. Alkaloid-specific homospermidine synthase retained all properties of deoxyhypusine synthase except binding the eIF5A precursor proteinSenecio vernalis12
Reversal of the deoxyhypusine synthesis reaction. Generation of spermidine or homospermidine from deoxyhypusine by deoxyhypusine synthaseHomo sapiens10

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
deoxyhypusine synthaseHomo sapiens1,4-diaminobutane1.0 mM NAD; 100 μg BSA; pure eIF5A([3H]Dhp)—1.118—pH 9.5, 37 °CPMID 12788913Open
deoxyhypusine synthaseHomo sapiens1,4-diaminobutane0.5 mM NAD; 50 μg BSA; spermidine (200 μM fixed)—0.203—pH 9.5, 37 °CPMID 12788913Open
deoxyhypusine synthaseHomo sapiensdeoxyhypusine-containing eIF5A intermediate (eIF5A([3H]Dhp), human eukaryotic initiation factor 5A carrying a [3H]deoxyhypusine residue)—8.6 × 10⁻⁴—pH 9.5, 37 °CPMID 12788913Open
deoxyhypusine synthaseHomo sapiensdeoxyhypusine-containing eIF5A intermediate (eIF5A([3H]Dhp), human eukaryotic initiation factor 5A carrying a [3H]deoxyhypusine residue)—6.6 × 10⁻⁴—pH 7.5, 37 °CPMID 12788913Open
deoxyhypusine synthaseHomo sapiensec-eIF5A, recombinant human eIF5A precursor protein (unmodified, lysine form)—0.00151—pH 9.5, 37 °CPMID 12788913Open
deoxyhypusine synthaseHomo sapienspropane-1,3-diamine—0.1058—pH 9.5, 37 °CPMID 12788913Open
deoxyhypusine synthaseHomo sapienspropane-1,3-diamine—0.0954—pH 7.5, 37 °CPMID 12788913Open
deoxyhypusine synthaseHomo sapiensspermidine1 mM DTT; 50 μg BSA; 20 min incubation—0.028—pH 9.5, 37 °CPMID 12788913Open
deoxyhypusine synthaseHomo sapiensspermidine1 mM DTT (Experimental Procedures); 30 min incubation—0.00726—pH 9.5, 37 °CPMID 12788913Open
deoxyhypusine synthaseHomo sapienssym-homospermidine (N-(4-aminobutyl)butane-1,4-diamine)—0.0193—pH 9.5, 37 °CPMID 12788913Open
deoxyhypusine synthaseSenecio vernaliseukaryotic initiation factor 5A precursor proteinstandard conditions: 0.1 M glycine-NaOH0.0022——pH 9.25, 30 °CPMID 12562768Open
deoxyhypusine synthaseSenecio vernaliseukaryotic initiation factor 5A precursor protein[14C]spermidine fixed at 10 µM; 0.1 M glycine-NaOH—0.0013—pH 9.25, 30 °CPMID 12562768Open
deoxyhypusine synthaseSenecio vernalisN-(3-aminopropyl)tetramethylene-1,4-diaminefixed putrescine 40 µM—0.005—pH 9.25, 30 °CPMID 12562768Open
deoxyhypusine synthaseSenecio vernalisN-(3-aminopropyl)tetramethylene-1,4-diaminefixed putrescine 400 µM—0.027—pH 9.25, 30 °CPMID 12562768Open
deoxyhypusine synthaseSenecio vernalisN-(3-aminopropyl)tetramethylene-1,4-diaminefixed putrescine 1000 µM—0.13—pH 9.25, 30 °CPMID 12562768Open
deoxyhypusine synthaseSenecio vernalisN-(4-aminobutyl)tetramethylene-1,4-diaminefixed putrescine 40 µM—0.007—pH 9.25, 30 °CPMID 12562768Open
deoxyhypusine synthaseSenecio vernalisN-(4-aminobutyl)tetramethylene-1,4-diaminefixed putrescine 400 µM—0.037—pH 9.25, 30 °CPMID 12562768Open
deoxyhypusine synthaseSenecio vernalisN-(4-aminobutyl)tetramethylene-1,4-diaminefixed putrescine 1000 µM—0.054—pH 9.25, 30 °CPMID 12562768Open
deoxyhypusine synthaseSenecio vernalisputrescine[14C]putrescine as labeled substrate; fixed spermidine 40 µM—0.31—pH 9.25, 30 °CPMID 12562768Open
deoxyhypusine synthaseSenecio vernalisputrescine[14C]putrescine as labeled substrate; apparent Km; Table II—0.431—pH 9.25, 30 °CPMID 12562768Open
deoxyhypusine synthaseSenecio vernalisputrescine[14C]putrescine as labeled substrate; apparent Km; Table II—1.057—pH 9.25, 30 °CPMID 12562768Open
deoxyhypusine synthaseSenecio vernalisputrescine400 µM [14C]putrescine; 400 µM spermidine; kcat per subunit0.032——pH 9.25, 30 °CPMID 12562768Open