Values CatLog read from this paper

Reversal of the deoxyhypusine synthesis reaction. Generation of spermidine or homospermidine from deoxyhypusine by deoxyhypusine synthase

PMID 12788913doi:10.1074/jbc.m304247200Open in Browse

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CatLog read 10 records (10 values) from this paper: deoxyhypusine synthase from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.5.1.46

Records read from this paper
EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsOpen record
deoxyhypusine synthaseHomo sapiens1,4-diaminobutane1.0 mM NAD; 100 μg BSA; pure eIF5A([3H]Dhp)—1.118—pH 9.5, 37 °COpen record: deoxyhypusine synthase, 1,4-diaminobutane
deoxyhypusine synthaseHomo sapiens1,4-diaminobutane0.5 mM NAD; 50 μg BSA; spermidine (200 μM fixed)—0.203—pH 9.5, 37 °COpen record: deoxyhypusine synthase, 1,4-diaminobutane
deoxyhypusine synthaseHomo sapiensdeoxyhypusine-containing eIF5A intermediate (eIF5A([3H]Dhp), human eukaryotic initiation factor 5A carrying a [3H]deoxyhypusine residue)—8.6 × 10⁻⁴—pH 9.5, 37 °COpen record: deoxyhypusine synthase, deoxyhypusine-containing eIF5A intermediate (eIF5A([3H]Dhp), human eukaryotic initiation factor 5A carrying a [3H]deoxyhypusine residue)
deoxyhypusine synthaseHomo sapiensdeoxyhypusine-containing eIF5A intermediate (eIF5A([3H]Dhp), human eukaryotic initiation factor 5A carrying a [3H]deoxyhypusine residue)—6.6 × 10⁻⁴—pH 7.5, 37 °COpen record: deoxyhypusine synthase, deoxyhypusine-containing eIF5A intermediate (eIF5A([3H]Dhp), human eukaryotic initiation factor 5A carrying a [3H]deoxyhypusine residue)
deoxyhypusine synthaseHomo sapiensec-eIF5A, recombinant human eIF5A precursor protein (unmodified, lysine form)—0.00151—pH 9.5, 37 °COpen record: deoxyhypusine synthase, ec-eIF5A, recombinant human eIF5A precursor protein (unmodified, lysine form)
deoxyhypusine synthaseHomo sapienspropane-1,3-diamine—0.1058—pH 9.5, 37 °COpen record: deoxyhypusine synthase, propane-1,3-diamine
deoxyhypusine synthaseHomo sapienspropane-1,3-diamine—0.0954—pH 7.5, 37 °COpen record: deoxyhypusine synthase, propane-1,3-diamine
deoxyhypusine synthaseHomo sapiensspermidine1 mM DTT; 50 μg BSA; 20 min incubation—0.028—pH 9.5, 37 °COpen record: deoxyhypusine synthase, spermidine
deoxyhypusine synthaseHomo sapiensspermidine1 mM DTT (Experimental Procedures); 30 min incubation—0.00726—pH 9.5, 37 °COpen record: deoxyhypusine synthase, spermidine
deoxyhypusine synthaseHomo sapienssym-homospermidine (N-(4-aminobutyl)butane-1,4-diamine)—0.0193—pH 9.5, 37 °COpen record: deoxyhypusine synthase, sym-homospermidine (N-(4-aminobutyl)butane-1,4-diamine)