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The molecular basis of the expansive substrate specificity of the antibiotic resistance enzyme aminoglycoside acetyltransferase-6'-aminoglycoside phosphotransferase-2". The role of ASP-99 as an…

PMID 12566434doi:10.1074/jbc.m211680200Open in Browse

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CatLog read 21 records (57 values) from this paper: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)).

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EC 2.3.1.82

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))	—	fortimicin A	0.28	0.0254	11	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)), fortimicin A
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))	—	neamine	0.43	0.0783	5.6	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)), neamine
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))	—	paromomycin	0.059	0.324	0.18	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)), paromomycin
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))variant D99A	—	acetyl-CoA	0.035	0.0453	0.79	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)) variant D99A, acetyl-CoA
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))variant D99A	—	fortimicin A	2 × 10⁻⁴	—	—	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)) variant D99A, fortimicin A
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))variant D99A	—	kanamycin A	0.033	0.126	0.26	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)) variant D99A, kanamycin A
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))variant D99A	—	neamine	0.029	0.443	0.065	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)) variant D99A, neamine
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))variant D99E	—	acetyl-CoA	0.11	0.017	6.5	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)) variant D99E, acetyl-CoA
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))variant D99E	—	fortimicin A	0.006	0.07	0.082	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)) variant D99E, fortimicin A
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))variant D99E	—	kanamycin A	0.19	0.102	1.8	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)) variant D99E, kanamycin A
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))variant D99E	—	neamine	0.066	0.229	0.29	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)) variant D99E, neamine
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))variant D99E	—	paromomycin	4 × 10⁻⁴	—	—	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)) variant D99E, paromomycin
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))variant D99N	—	acetyl-CoA	0.17	0.0399	4.4	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)) variant D99N, acetyl-CoA
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))variant D99N	—	fortimicin A	8 × 10⁻⁴	—	—	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)) variant D99N, fortimicin A
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))variant D99N	—	kanamycin A	0.038	0.0902	0.43	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)) variant D99N, kanamycin A
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))variant D99N	—	neamine	0.028	0.509	0.055	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)) variant D99N, neamine
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))variant Y96F	—	acetyl-CoA	1.3	0.0736	18	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)) variant Y96F, acetyl-CoA
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))variant Y96F	—	fortimicin A	0.45	0.0435	10	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)) variant Y96F, fortimicin A
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))variant Y96F	—	kanamycin A	1.1	0.079	13	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)) variant Y96F, kanamycin A
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))variant Y96F	—	neamine	1.3	0.326	3.9	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)) variant Y96F, neamine
AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″))variant Y96F	—	paromomycin	0.026	0.771	0.033	pH 7.5, 37 °C	Open record: AAC(6′)-Ie (aminoglycoside 6′-N/O-acetyltransferase domain of bifunctional AAC(6′)-APH(2″)) variant Y96F, paromomycin