CatLogenzyme kinetics catalog

Values CatLog read from this paper

Enzyme catalysis via control of activation entropy: site-directed mutagenesis of 6,7-dimethyl-8-ribityllumazine synthase

PMID 12581640doi:10.1016/s0022-2836(02)01473-0Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 37 records (56 values) from this paper: 6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase) from Bacillus subtilis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 19 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.5.1.78

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)Bacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.05570.054661.02 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)Bacillus subtilis5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione—0.00857—pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant A56SBacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.04530.070150.646 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant A56SBacillus subtilis5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione—0.0042—pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant D138ABacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.0510.063640.80 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant D138ABacillus subtilis5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione—0.00996—pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant E58QBacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.03910.1460.268 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant E58QBacillus subtilis5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione—0.1732—pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant F113SBacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.00380.066450.057 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant F113SBacillus subtilis5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione—0.2785—pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant F22DBacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.00810.67480.012 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant F22SBacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.02630.13730.191 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant F22SBacillus subtilis5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione—0.1405—pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant F22VBacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.01470.12440.118 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant F22VBacillus subtilis5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione—0.01072—pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant F22WBacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.02440.059830.408 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant F22WBacillus subtilis5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione—0.00612—pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant F57SBacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.02440.080070.305 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant F57SBacillus subtilis5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione—0.03449—pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant H88ABacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.00670.060120.11 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant H88ABacillus subtilis5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione—0.1465—pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant H88KBacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.00220.042190.052 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant H88KBacillus subtilis5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione—0.05557—pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant K131NBacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.00540.28250.019 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant K131NBacillus subtilis5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione—0.03552—pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant K131RBacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.01660.049980.332 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant K131RBacillus subtilis5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione—0.01379—pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant K135ABacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.01220.16660.0732 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant K135ABacillus subtilis5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione—0.01148—pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant N23SBacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.01220.058080.210 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant N23SBacillus subtilis5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione—0.01191—pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant R127HBacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.03883.1380.0124 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant R127HBacillus subtilis5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione—0.08492—pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant S142LBacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.03470.090520.383 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant S142LBacillus subtilis5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione—0.01148—pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant T80VBacillus subtilis3,4-dihydroxy-2-butanone 4-phosphate0.03070.041960.732 calc.pH 7, 37 °COpen
6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase)variant T80VBacillus subtilis5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione—0.00406—pH 7, 37 °COpen