Values CatLog read from this paper
Heat effect on the structure and activity of the recombinant glutamate dehydrogenase from a hyperthermophilic archaeon Pyrococcus horikoshii
PMID 12590923doi:10.1016/s0003-9861(02)00713-0Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (12 values) from this paper: glutamate dehydrogenase (Pho-GDH) from Pyrococcus horikoshii.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glutamate dehydrogenase (Pho-GDH) | Pyrococcus horikoshii | 2-oxoglutarate | 165.3 | 0.53 | 310 calc. | pH 7.6, 90 °C | Open record: glutamate dehydrogenase (Pho-GDH), 2-oxoglutarate |
| glutamate dehydrogenase (Pho-GDH) | Pyrococcus horikoshii | ammonia | 43.8 | 2.82 | 15.5 calc. | pH 7.6, 90 °C | Open record: glutamate dehydrogenase (Pho-GDH), ammonia |
| glutamate dehydrogenase (Pho-GDH) | Pyrococcus horikoshii | L-glutamate | 121.2 | 0.6 | 200 calc. | pH 7.6, 90 °C | Open record: glutamate dehydrogenase (Pho-GDH), L-glutamate |
| glutamate dehydrogenase (Pho-GDH) | Pyrococcus horikoshii | NADH | 374.5 | 0.083 | 4.5 × 10³ calc. | pH 7.6, 90 °C | Open record: glutamate dehydrogenase (Pho-GDH), NADH |
| glutamate dehydrogenase (Pho-GDH) | Pyrococcus horikoshii | NADP+ | 399.6 | 0.025 | 1.6 × 10⁴ calc. | pH 7.6, 90 °C | Open record: glutamate dehydrogenase (Pho-GDH), NADP+ |
| glutamate dehydrogenase (Pho-GDH) | Pyrococcus horikoshii | NADPH | 387.5 | 0.037 | 1.0 × 10⁴ calc. | pH 7.6, 90 °C | Open record: glutamate dehydrogenase (Pho-GDH), NADPH |