Values CatLog read from this paper
Site-directed mutagenesis of a loop at the active site of E1 (alpha2beta2) of the pyruvate dehydrogenase complex. A possible common sequence motif
PMID 12603319doi:10.1046/j.1432-1033.2003.03444.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (60 values) from this paper: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex from Bacillus stearothermophilus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex | Bacillus stearothermophilus | pyruvate | 0.48 | 0.0011 | 427 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex, pyruvate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex | Bacillus stearothermophilus | thiamin diphosphate | 0.46 | 0.023 | 20 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex, thiamin diphosphate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant D276A | Bacillus stearothermophilus | pyruvate | 0.71 | 0.0111 | 64 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex variant D276A, pyruvate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant D276A | Bacillus stearothermophilus | thiamin diphosphate | 0.91 | 0.021 | 42 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex variant D276A, thiamin diphosphate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant F266A | Bacillus stearothermophilus | pyruvate | 0.3 | 0.0012 | 246 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex variant F266A, pyruvate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant F266A | Bacillus stearothermophilus | thiamin diphosphate | 0.35 | 0.021 | 17 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex variant F266A, thiamin diphosphate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant R267A | Bacillus stearothermophilus | pyruvate | 1.62 | 0.291 | 6 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex variant R267A, pyruvate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant R267A | Bacillus stearothermophilus | thiamin diphosphate | 1.25 | 5.6 × 10⁻⁴ | 2232 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex variant R267A, thiamin diphosphate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant R282A | Bacillus stearothermophilus | pyruvate | 0.55 | 0.0088 | 63 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex variant R282A, pyruvate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant R282A | Bacillus stearothermophilus | thiamin diphosphate | 0.62 | 0.0052 | 121 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex variant R282A, thiamin diphosphate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant S283C | Bacillus stearothermophilus | pyruvate | 0.38 | 0.0021 | 186 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex variant S283C, pyruvate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant S283C | Bacillus stearothermophilus | thiamin diphosphate | 0.45 | 0.013 | 33 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex variant S283C, thiamin diphosphate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant Y281A | Bacillus stearothermophilus | pyruvate | 0.82 | 0.063 | 13 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex variant Y281A, pyruvate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant Y281A | Bacillus stearothermophilus | thiamin diphosphate | 0.97 | 0.0055 | 177 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex variant Y281A, thiamin diphosphate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant Y281A/R282A | Bacillus stearothermophilus | pyruvate | 0.96 | 0.056 | 17 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex variant Y281A/R282A, pyruvate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant Y281A/R282A | Bacillus stearothermophilus | thiamin diphosphate | 0.98 | 0.003 | 326 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex variant Y281A/R282A, thiamin diphosphate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant Y281A/R282A/S283A | Bacillus stearothermophilus | pyruvate | 1.23 | 0.049 | 25 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex variant Y281A/R282A/S283A, pyruvate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant Y281A/R282A/S283A | Bacillus stearothermophilus | thiamin diphosphate | 1.39 | 0.0029 | 481 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex variant Y281A/R282A/S283A, thiamin diphosphate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant Y281S/R282S | Bacillus stearothermophilus | pyruvate | 1.21 | 0.066 | 19 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex variant Y281S/R282S, pyruvate |
| pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant Y281S/R282S | Bacillus stearothermophilus | thiamin diphosphate | 1.3 | 0.0027 | 475 | pH 7, 30 °C | Open record: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex variant Y281S/R282S, thiamin diphosphate |