CatLogenzyme kinetics catalog

EC 1.2.4.1: pyruvate dehydrogenase (acetyl-transferring)

OxidoreductasesOpen in Browse

CatLog read 44 records for EC 1.2.4.1 from 6 papers, for 4 organisms. Names in these papers: pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complex, pyruvate dehydrogenase complex E1 component (PDHc-E1), pyruvate dehydrogenase (E1 subunit of the pyruvate dehydrogenase multienzyme complex), pyruvate dehydrogenase complex and 1 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 63 records for EC 1.2.4.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.2.4.1 in Browse.

Papers

PaperOrganismsRecords
Site-directed mutagenesis of a loop at the active site of E1 (alpha2beta2) of the pyruvate dehydrogenase complex. A possible common sequence motifBacillus stearothermophilus20
Glutamate 636 of the Escherichia coli pyruvate dehydrogenase-E1 participates in active center communication and behaves as an engineered acetolactate synthase with unusual stereoselectivityEscherichia coli13
Inhibition of the Escherichia coli pyruvate dehydrogenase complex E1 subunit and its tyrosine 177 variants by thiamin 2-thiazolone and thiamin 2-thiothiazolone diphosphates. Evidence for reversible…Escherichia coli6
Reaction mechanism of the heterotetrameric (alpha2beta2) E1 component of 2-oxo acid dehydrogenase multienzyme complexesBacillus stearothermophilus2
Methylcitrate synthase from Aspergillus fumigatus. Propionyl-CoA affects polyketide synthesis, growth and morphology of conidiaAspergillus fumigatus2
The E2 domain of OdhA of Corynebacterium glutamicum has succinyltransferase activity dependent on lipoyl residues of the acetyltransferase AceFCorynebacterium glutamicum1

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
AceE (E1p, pyruvate dehydrogenase E1)Corynebacterium glutamicumpyruvate—0.066—pH 7.5PMID 20675489Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexBacillus stearothermophiluspyruvate0.480.0011427pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexBacillus stearothermophilusthiamin diphosphate0.460.02320pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant D276ABacillus stearothermophiluspyruvate0.710.011164pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant D276ABacillus stearothermophilusthiamin diphosphate0.910.02142pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant F266ABacillus stearothermophiluspyruvate0.30.0012246pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant F266ABacillus stearothermophilusthiamin diphosphate0.350.02117pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant H271A (E1α)Bacillus stearothermophiluspyruvate3.80.4429pH 7, 30 °CPMID 12795594Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant H271A (E1α)Bacillus stearothermophilusthiamin diphosphate2.490.0026950pH 7, 30 °CPMID 12795594Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant R267ABacillus stearothermophiluspyruvate1.620.2916pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant R267ABacillus stearothermophilusthiamin diphosphate1.255.6 × 10⁻⁴2232pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant R282ABacillus stearothermophiluspyruvate0.550.008863pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant R282ABacillus stearothermophilusthiamin diphosphate0.620.0052121pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant S283CBacillus stearothermophiluspyruvate0.380.0021186pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant S283CBacillus stearothermophilusthiamin diphosphate0.450.01333pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant Y281ABacillus stearothermophiluspyruvate0.820.06313pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant Y281ABacillus stearothermophilusthiamin diphosphate0.970.0055177pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant Y281A/R282ABacillus stearothermophiluspyruvate0.960.05617pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant Y281A/R282ABacillus stearothermophilusthiamin diphosphate0.980.003326pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant Y281A/R282A/S283ABacillus stearothermophiluspyruvate1.230.04925pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant Y281A/R282A/S283ABacillus stearothermophilusthiamin diphosphate1.390.0029481pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant Y281S/R282SBacillus stearothermophiluspyruvate1.210.06619pH 7, 30 °CPMID 12603319Open
pyruvate decarboxylase (E1, α2β2) component of the pyruvate dehydrogenase complexvariant Y281S/R282SBacillus stearothermophilusthiamin diphosphate1.30.0027475pH 7, 30 °CPMID 12603319Open
pyruvate dehydrogenase (E1 subunit of the pyruvate dehydrogenase multienzyme complex)Escherichia colipyruvateTable I28.89——pH 8, 30 °CPMID 11583990Open
pyruvate dehydrogenase (E1 subunit of the pyruvate dehydrogenase multienzyme complex)Escherichia colipyruvateTable II—0.51556pH 8, 30 °CPMID 11583990Open
pyruvate dehydrogenase (E1 subunit of the pyruvate dehydrogenase multienzyme complex)variant Y177AEscherichia colipyruvateTable I3.31——pH 8, 30 °CPMID 11583990Open
pyruvate dehydrogenase (E1 subunit of the pyruvate dehydrogenase multienzyme complex)variant Y177AEscherichia colipyruvateTable II—0.2812pH 8, 30 °CPMID 11583990Open
pyruvate dehydrogenase (E1 subunit of the pyruvate dehydrogenase multienzyme complex)variant Y177AEscherichia colithiamin diphosphate—0.00665498pH 8, 30 °CPMID 11583990Open
pyruvate dehydrogenase (E1 subunit of the pyruvate dehydrogenase multienzyme complex)variant Y177FEscherichia colipyruvate—0.531—pH 8, 30 °CPMID 11583990Open
pyruvate dehydrogenase complexAspergillus fumigatuscoenzyme A—0.0085—PMID 16008561Open
pyruvate dehydrogenase complexAspergillus fumigatuscoenzyme Awith 0.15 mM propionyl-CoA (competitive inhibitor)—0.0325—PMID 16008561Open
pyruvate dehydrogenase complex E1 component (PDHc-E1)Escherichia colipyruvateTable I98.7——pH 8, 30 °CPMID 15802265Open
pyruvate dehydrogenase complex E1 component (PDHc-E1)Escherichia colipyruvate1.28——PMID 15802265Open
pyruvate dehydrogenase complex E1 component (PDHc-E1)Escherichia colipyruvateTable II—0.515192pH 8, 30 °CPMID 15802265Open
pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636AEscherichia colipyruvateTable I2.41——pH 8, 30 °CPMID 15802265Open
pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636AEscherichia colipyruvate0.48——PMID 15802265Open
pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636AEscherichia colipyruvateTable II—0.23710pH 8, 30 °CPMID 15802265Open
pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636AEscherichia colipyruvate2.23——pH 7, 30 °CPMID 15802265Open
pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636AEscherichia colipyruvateacetolactate1.01——pH 7PMID 15802265Open
pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636AEscherichia colipyruvateacetate (oxygenase side reaction)0.07——pH 7PMID 15802265Open
pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636QEscherichia colipyruvateTable I25.9——pH 8, 30 °CPMID 15802265Open
pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636QEscherichia colipyruvate0.45——PMID 15802265Open
pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636QEscherichia colipyruvateTable II—0.205126pH 8, 30 °CPMID 15802265Open
pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636QEscherichia colipyruvate1.66——pH 7, 30 °CPMID 15802265Open