Values CatLog read from this paper
Glutamate 636 of the Escherichia coli pyruvate dehydrogenase-E1 participates in active center communication and behaves as an engineered acetolactate synthase with unusual stereoselectivity
PMID 15802265doi:10.1074/jbc.m502691200Open in Browse
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CatLog read 13 records (16 values) from this paper: pyruvate dehydrogenase complex E1 component (PDHc-E1) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| pyruvate dehydrogenase complex E1 component (PDHc-E1) | Escherichia coli | pyruvateTable I | 98.7 | — | — | pH 8, 30 °C | Open record: pyruvate dehydrogenase complex E1 component (PDHc-E1), pyruvate |
| pyruvate dehydrogenase complex E1 component (PDHc-E1) | Escherichia coli | pyruvate | 1.28 | — | — | Open record: pyruvate dehydrogenase complex E1 component (PDHc-E1), pyruvate | |
| pyruvate dehydrogenase complex E1 component (PDHc-E1) | Escherichia coli | pyruvateTable II | — | 0.515 | 192 | pH 8, 30 °C | Open record: pyruvate dehydrogenase complex E1 component (PDHc-E1), pyruvate |
| pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636A | Escherichia coli | pyruvateTable I | 2.41 | — | — | pH 8, 30 °C | Open record: pyruvate dehydrogenase complex E1 component (PDHc-E1) variant E636A, pyruvate |
| pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636A | Escherichia coli | pyruvate | 0.48 | — | — | Open record: pyruvate dehydrogenase complex E1 component (PDHc-E1) variant E636A, pyruvate | |
| pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636A | Escherichia coli | pyruvateTable II | — | 0.237 | 10 | pH 8, 30 °C | Open record: pyruvate dehydrogenase complex E1 component (PDHc-E1) variant E636A, pyruvate |
| pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636A | Escherichia coli | pyruvate | 2.23 | — | — | pH 7, 30 °C | Open record: pyruvate dehydrogenase complex E1 component (PDHc-E1) variant E636A, pyruvate |
| pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636A | Escherichia coli | pyruvateacetolactate | 1.01 | — | — | pH 7 | Open record: pyruvate dehydrogenase complex E1 component (PDHc-E1) variant E636A, pyruvate |
| pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636A | Escherichia coli | pyruvateacetate (oxygenase side reaction) | 0.07 | — | — | pH 7 | Open record: pyruvate dehydrogenase complex E1 component (PDHc-E1) variant E636A, pyruvate |
| pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636Q | Escherichia coli | pyruvateTable I | 25.9 | — | — | pH 8, 30 °C | Open record: pyruvate dehydrogenase complex E1 component (PDHc-E1) variant E636Q, pyruvate |
| pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636Q | Escherichia coli | pyruvate | 0.45 | — | — | Open record: pyruvate dehydrogenase complex E1 component (PDHc-E1) variant E636Q, pyruvate | |
| pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636Q | Escherichia coli | pyruvateTable II | — | 0.205 | 126 | pH 8, 30 °C | Open record: pyruvate dehydrogenase complex E1 component (PDHc-E1) variant E636Q, pyruvate |
| pyruvate dehydrogenase complex E1 component (PDHc-E1)variant E636Q | Escherichia coli | pyruvate | 1.66 | — | — | pH 7, 30 °C | Open record: pyruvate dehydrogenase complex E1 component (PDHc-E1) variant E636Q, pyruvate |