CatLogenzyme kinetics catalog

Values CatLog read from this paper

Steroid-binding site residues dictate optimal substrate positioning in rat 3alpha-hydroxysteroid dehydrogenase (3alpha-HSD or AKR1C9)

PMID 12604226doi:10.1016/s0009-2797(02)00176-xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 35 records (92 values) from this paper: 3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9) from Rattus norvegicus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.

EC 1.1.1.50

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)Rattus norvegicus5α-Androstan-3α-ol-17-one2.70.07535.3pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)Rattus norvegicus5α-Pregnan-3α-ol-20-one0.350.0060357.5pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)Rattus norvegicus5β-Androstan-3α-ol-17-one2.570.07435pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)Rattus norvegicus5β-Pregnan-3α-ol-20-one1.420.026453.8pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant F118ARattus norvegicus5α-Androstan-3α-ol-17-one0.01170.3670.033pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant F118ARattus norvegicus5α-Pregnan-3α-ol-20-one0.00520.008530.67pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant F118ARattus norvegicus5β-Androstan-3α-ol-17-one0.04020.7210.05pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant F129ARattus norvegicus5α-Androstan-3α-ol-17-one0.0150.2680.05pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant F129ARattus norvegicus5α-Pregnan-3α-ol-20-one0.00350.004510.83pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant F129ARattus norvegicus5β-Androstan-3α-ol-17-one0.02470.3970.067pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant F129ARattus norvegicus5β-Pregnan-3α-ol-20-one——0.05pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant L54ARattus norvegicus5α-Androstan-3α-ol-17-one0.1620.2050.83pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant L54ARattus norvegicus5α-Pregnan-3α-ol-20-one0.00930.006331.5pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant L54ARattus norvegicus5β-Androstan-3α-ol-17-one——0.5pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant L54ARattus norvegicus5β-Pregnan-3α-ol-20-one——0.33pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant N306ARattus norvegicus5α-Androstan-3α-ol-17-one0.04030.1860.22pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant N306ARattus norvegicus5α-Pregnan-3α-ol-20-one0.00970.0120.83pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant N306ARattus norvegicus5β-Androstan-3α-ol-17-one0.1920.2880.67pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant N306ARattus norvegicus5β-Pregnan-3α-ol-20-one0.10.1210.83pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant T226ARattus norvegicus5α-Androstan-3α-ol-17-one1.90.08822pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant T226ARattus norvegicus5α-Pregnan-3α-ol-20-one0.4380.0078455.8pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant T226ARattus norvegicus5β-Androstan-3α-ol-17-one20.0728.7pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant T226ARattus norvegicus5β-Pregnan-3α-ol-20-one2.620.1221.8pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant T24ARattus norvegicus5α-Androstan-3α-ol-17-one2.930.12224.2pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant T24ARattus norvegicus5α-Pregnan-3α-ol-20-one0.250.0061539.5pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant T24ARattus norvegicus5β-Androstan-3α-ol-17-one3.30.27612pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant T24ARattus norvegicus5β-Pregnan-3α-ol-20-one——12.5pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant W227ARattus norvegicus5α-Androstan-3α-ol-17-one0.00920.250.033pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant W227ARattus norvegicus5α-Pregnan-3α-ol-20-one0.00130.006050.22 calc.pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant W227ARattus norvegicus5β-Androstan-3α-ol-17-one0.0480.7550.067pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant W227ARattus norvegicus5β-Pregnan-3α-ol-20-one0.0470.1320.33pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant Y310ARattus norvegicus5α-Androstan-3α-ol-17-one0.08120.5290.15pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant Y310ARattus norvegicus5α-Pregnan-3α-ol-20-one0.0180.006362.8pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant Y310ARattus norvegicus5β-Androstan-3α-ol-17-one——1.8pH 7, 37 °COpen
3α-hydroxysteroid dehydrogenase (3α-HSD or AKR1C9)variant Y310ARattus norvegicus5β-Pregnan-3α-ol-20-one——2.2pH 7, 37 °COpen