Values CatLog read from this paper
Mutagenesis of cysteine 280 of the reverse transcriptase of human immunodeficiency virus type-1: the effects on the ribonuclease H activity
PMID 12614605doi:10.1016/s0022-2836(03)00052-4Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 7 records (21 values) from this paper: HIV-1 reverse transcriptase (RNase H activity) from human immunodeficiency virus type 1.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| HIV-1 reverse transcriptase (RNase H activity) | human immunodeficiency virus type 1 | polyriboadenylic acid annealed to polydeoxythymidylic acid (poly(rA)·poly(dT) RNA·DNA heteroduplex) | 0.031 | 0.003 | 10.3 | 37 °C | Open record: HIV-1 reverse transcriptase (RNase H activity), polyriboadenylic acid annealed to polydeoxythymidylic acid (poly(rA)·poly(dT) RNA·DNA heteroduplex) |
| HIV-1 reverse transcriptase (RNase H activity)variant p66/p51C280P | human immunodeficiency virus type 1 | polyriboadenylic acid annealed to polydeoxythymidylic acid (poly(rA)·poly(dT) RNA·DNA heteroduplex) | 0.024 | 0.0257 | 0.93 | 37 °C | Open record: HIV-1 reverse transcriptase (RNase H activity) variant p66/p51C280P, polyriboadenylic acid annealed to polydeoxythymidylic acid (poly(rA)·poly(dT) RNA·DNA heteroduplex) |
| HIV-1 reverse transcriptase (RNase H activity)variant p66/p51C280W | human immunodeficiency virus type 1 | polyriboadenylic acid annealed to polydeoxythymidylic acid (poly(rA)·poly(dT) RNA·DNA heteroduplex) | 0.024 | 0.0383 | 0.62 | 37 °C | Open record: HIV-1 reverse transcriptase (RNase H activity) variant p66/p51C280W, polyriboadenylic acid annealed to polydeoxythymidylic acid (poly(rA)·poly(dT) RNA·DNA heteroduplex) |
| HIV-1 reverse transcriptase (RNase H activity)variant p66C280P/p51 | human immunodeficiency virus type 1 | polyriboadenylic acid annealed to polydeoxythymidylic acid (poly(rA)·poly(dT) RNA·DNA heteroduplex) | 0.025 | 0.0177 | 1.46 | 37 °C | Open record: HIV-1 reverse transcriptase (RNase H activity) variant p66C280P/p51, polyriboadenylic acid annealed to polydeoxythymidylic acid (poly(rA)·poly(dT) RNA·DNA heteroduplex) |
| HIV-1 reverse transcriptase (RNase H activity)variant p66C280P/p51C280P | human immunodeficiency virus type 1 | polyriboadenylic acid annealed to polydeoxythymidylic acid (poly(rA)·poly(dT) RNA·DNA heteroduplex) | 0.029 | 0.0553 | 0.52 | 37 °C | Open record: HIV-1 reverse transcriptase (RNase H activity) variant p66C280P/p51C280P, polyriboadenylic acid annealed to polydeoxythymidylic acid (poly(rA)·poly(dT) RNA·DNA heteroduplex) |
| HIV-1 reverse transcriptase (RNase H activity)variant p66C280W/p51 | human immunodeficiency virus type 1 | polyriboadenylic acid annealed to polydeoxythymidylic acid (poly(rA)·poly(dT) RNA·DNA heteroduplex) | 0.029 | 0.0318 | 0.91 | 37 °C | Open record: HIV-1 reverse transcriptase (RNase H activity) variant p66C280W/p51, polyriboadenylic acid annealed to polydeoxythymidylic acid (poly(rA)·poly(dT) RNA·DNA heteroduplex) |
| HIV-1 reverse transcriptase (RNase H activity)variant p66C280W/p51C280W | human immunodeficiency virus type 1 | polyriboadenylic acid annealed to polydeoxythymidylic acid (poly(rA)·poly(dT) RNA·DNA heteroduplex) | 0.034 | 0.0663 | 0.51 | 37 °C | Open record: HIV-1 reverse transcriptase (RNase H activity) variant p66C280W/p51C280W, polyriboadenylic acid annealed to polydeoxythymidylic acid (poly(rA)·poly(dT) RNA·DNA heteroduplex) |