CatLogenzyme kinetics catalog

Values CatLog read from this paper

The kinetic properties of various R258 mutants of deacetoxycephalosporin C synthase

PMID 12631288doi:10.1046/j.1432-1033.2003.03500.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 28 records (84 values) from this paper: deacetoxycephalosporin C synthase (DAOCS) from Streptomyces clavuligerus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.14.20.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
deacetoxycephalosporin C synthase (DAOCS)Streptomyces clavuligerusampicillin0.0142.60.0054pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)Streptomyces clavuligeruspenicillin G0.0211.10.019pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258AStreptomyces clavuligerusampicillin2-oxohexanoate; 2-oxohexanoate (2-OH)0.0084.20.0018pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258AStreptomyces clavuligerusampicillin2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP)0.0114.50.0024pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258AStreptomyces clavuligeruspenicillin G2-oxohexanoate; 2-oxohexanoate (2-OH)0.0130.790.017pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258AStreptomyces clavuligeruspenicillin G2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP)0.021.80.011pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258FStreptomyces clavuligerusampicillin2-oxohexanoate; 2-oxohexanoate (2-OH)0.0121.90.0063pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258FStreptomyces clavuligerusampicillin2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP)0.0110.790.014pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258FStreptomyces clavuligeruspenicillin G2-oxohexanoate; 2-oxohexanoate (2-OH)0.0241.60.015pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258FStreptomyces clavuligeruspenicillin G2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP)0.0250.840.03pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258HStreptomyces clavuligerusampicillin2-oxohexanoate; 2-oxohexanoate (2-OH)0.0074.20.0017pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258HStreptomyces clavuligerusampicillin2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP)0.00534.10.0013pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258HStreptomyces clavuligeruspenicillin G2-oxohexanoate; 2-oxohexanoate (2-OH)0.01330.0043pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258HStreptomyces clavuligeruspenicillin G2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP)0.0141.50.0093pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258KStreptomyces clavuligerusampicillin2-oxoglutarate; 2-oxoglutarate (2-OG)0.011138 × 10⁻⁴pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258KStreptomyces clavuligerusampicillin2-oxohexanoate; 2-oxohexanoate (2-OH)0.009244 × 10⁻⁴pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258KStreptomyces clavuligerusampicillin2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP)0.013159 × 10⁻⁴pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258KStreptomyces clavuligeruspenicillin G2-oxoglutarate; 2-oxoglutarate (2-OG)0.016.40.0016pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258KStreptomyces clavuligeruspenicillin G2-oxohexanoate; 2-oxohexanoate (2-OH)0.0014.70.0021pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258KStreptomyces clavuligeruspenicillin G2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP)0.0270.0029pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258LStreptomyces clavuligerusampicillin2-oxohexanoate; 2-oxohexanoate (2-OH)0.0122.60.0046pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258LStreptomyces clavuligerusampicillin2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP)0.00861.30.0066pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258LStreptomyces clavuligeruspenicillin G2-oxohexanoate; 2-oxohexanoate (2-OH)0.0191.70.011pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258LStreptomyces clavuligeruspenicillin G2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP)0.021.20.017pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258QStreptomyces clavuligerusampicillin2-oxohexanoate; 2-oxohexanoate (2-OH)0.0176.60.0026pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258QStreptomyces clavuligerusampicillin2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP)0.0143.30.0042pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258QStreptomyces clavuligeruspenicillin G2-oxohexanoate; 2-oxohexanoate (2-OH)0.010.790.013pH 7.5Open
deacetoxycephalosporin C synthase (DAOCS)variant R258QStreptomyces clavuligeruspenicillin G2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP)0.0211.50.014pH 7.5Open