EC 1.14.20.1: deacetoxycephalosporin-C synthase
OxidoreductasesOpen in Browse
CatLog read 105 records for EC 1.14.20.1 from 5 papers, for 2 organisms. Names in these papers: deacetoxycephalosporin C synthase (DAOCS), deacetoxycephalosporin C synthase (ScDAOCS), deacetoxycephalosporin C synthase (DAOCS, expandase), deacetoxycephalosporin/deacetylcephalosporin C synthase (DAOC/DACS).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 82 records for EC 1.14.20.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.14.20.1 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Relevant double mutations in bioengineered Streptomyces clavuligerus deacetoxycephalosporin C synthase result in higher binding specificities which improve penicillin bioconversion | Streptomyces clavuligerus | 34 |
| The kinetic properties of various R258 mutants of deacetoxycephalosporin C synthase | Streptomyces clavuligerus | 28 |
| Directed evolution of Streptomyces clavuligerus deacetoxycephalosporin C synthase for enhancement of penicillin G expansion | Streptomyces clavuligerus | 22 |
| Engineering Streptomyces clavuligerus deacetoxycephalosporin C synthase for optimal ring expansion activity toward penicillin G | Streptomyces clavuligerus | 16 |
| Controlling the substrate selectivity of deacetoxycephalosporin/deacetylcephalosporin C synthase | Cephalosporium acremonium | 5 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| deacetoxycephalosporin C synthase (DAOCS) | Streptomyces clavuligerus | ampicillin | 0.014 | 2.6 | 0.0054 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS), ampicillin |
| deacetoxycephalosporin C synthase (DAOCS) | Streptomyces clavuligerus | penicillin G | 0.021 | 1.1 | 0.019 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS), penicillin G |
| deacetoxycephalosporin C synthase (DAOCS) | Streptomyces clavuligerus | penicillin G | 0.0453 | 2.58 | 0.018 | pH 7.5, 30 °C | PMID 12676714 | Open record: deacetoxycephalosporin C synthase (DAOCS), penicillin G |
| deacetoxycephalosporin C synthase (DAOCS) | Streptomyces clavuligerus | penicillin N | 0.307 | 0.014 | 22 | pH 7.5, 30 °C | PMID 12676714 | Open record: deacetoxycephalosporin C synthase (DAOCS), penicillin N |
| deacetoxycephalosporin C synthase (DAOCS)variant C281Y (YS53) | Streptomyces clavuligerus | penicillin G | 0.0744 | 0.68 | 0.109 | pH 7.5, 30 °C | PMID 12676714 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant C281Y (YS53), penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant C281Y (YS53) | Streptomyces clavuligerus | penicillin N | 0.273 | 0.006 | 47 | pH 7.5, 30 °C | PMID 12676714 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant C281Y (YS53), penicillin N |
| deacetoxycephalosporin C synthase (DAOCS)variant G79E (YS59) | Streptomyces clavuligerus | penicillin G | 0.0315 | 0.75 | 0.042 | pH 7.5, 30 °C | PMID 12676714 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant G79E (YS59), penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant G79E (YS59) | Streptomyces clavuligerus | penicillin N | 0.178 | 0.009 | 20 | pH 7.5, 30 °C | PMID 12676714 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant G79E (YS59), penicillin N |
| deacetoxycephalosporin C synthase (DAOCS)variant I305L (YS8) | Streptomyces clavuligerus | penicillin G | 0.0759 | 0.66 | 0.115 | pH 7.5, 30 °C | PMID 12676714 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant I305L (YS8), penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant I305L (YS8) | Streptomyces clavuligerus | penicillin N | 0.284 | 0.006 | 44 | pH 7.5, 30 °C | PMID 12676714 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant I305L (YS8), penicillin N |
| deacetoxycephalosporin C synthase (DAOCS)variant I305M (YS11) | Streptomyces clavuligerus | penicillin G | 0.1452 | 0.75 | 0.194 | pH 7.5, 30 °C | PMID 12676714 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant I305M (YS11), penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant I305M (YS11) | Streptomyces clavuligerus | penicillin N | 0.31 | 0.012 | 26 | pH 7.5, 30 °C | PMID 12676714 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant I305M (YS11), penicillin N |
| deacetoxycephalosporin C synthase (DAOCS)variant N304K (YS12) | Streptomyces clavuligerus | penicillin G | 0.0564 | 0.22 | 0.256 | pH 7.5, 30 °C | PMID 12676714 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant N304K (YS12), penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant N304K (YS12) | Streptomyces clavuligerus | penicillin N | 0.366 | 0.004 | 92 | pH 7.5, 30 °C | PMID 12676714 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant N304K (YS12), penicillin N |
| deacetoxycephalosporin C synthase (DAOCS)variant R258A | Streptomyces clavuligerus | ampicillin2-oxohexanoate; 2-oxohexanoate (2-OH) | 0.008 | 4.2 | 0.0018 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258A, ampicillin |
| deacetoxycephalosporin C synthase (DAOCS)variant R258A | Streptomyces clavuligerus | ampicillin2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP) | 0.011 | 4.5 | 0.0024 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258A, ampicillin |
| deacetoxycephalosporin C synthase (DAOCS)variant R258A | Streptomyces clavuligerus | penicillin G2-oxohexanoate; 2-oxohexanoate (2-OH) | 0.013 | 0.79 | 0.017 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258A, penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant R258A | Streptomyces clavuligerus | penicillin G2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP) | 0.02 | 1.8 | 0.011 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258A, penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant R258F | Streptomyces clavuligerus | ampicillin2-oxohexanoate; 2-oxohexanoate (2-OH) | 0.012 | 1.9 | 0.0063 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258F, ampicillin |
| deacetoxycephalosporin C synthase (DAOCS)variant R258F | Streptomyces clavuligerus | ampicillin2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP) | 0.011 | 0.79 | 0.014 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258F, ampicillin |
| deacetoxycephalosporin C synthase (DAOCS)variant R258F | Streptomyces clavuligerus | penicillin G2-oxohexanoate; 2-oxohexanoate (2-OH) | 0.024 | 1.6 | 0.015 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258F, penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant R258F | Streptomyces clavuligerus | penicillin G2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP) | 0.025 | 0.84 | 0.03 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258F, penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant R258H | Streptomyces clavuligerus | ampicillin2-oxohexanoate; 2-oxohexanoate (2-OH) | 0.007 | 4.2 | 0.0017 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258H, ampicillin |
| deacetoxycephalosporin C synthase (DAOCS)variant R258H | Streptomyces clavuligerus | ampicillin2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP) | 0.0053 | 4.1 | 0.0013 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258H, ampicillin |
| deacetoxycephalosporin C synthase (DAOCS)variant R258H | Streptomyces clavuligerus | penicillin G2-oxohexanoate; 2-oxohexanoate (2-OH) | 0.013 | 3 | 0.0043 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258H, penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant R258H | Streptomyces clavuligerus | penicillin G2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP) | 0.014 | 1.5 | 0.0093 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258H, penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant R258K | Streptomyces clavuligerus | ampicillin2-oxoglutarate; 2-oxoglutarate (2-OG) | 0.011 | 13 | 8 × 10⁻⁴ | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258K, ampicillin |
| deacetoxycephalosporin C synthase (DAOCS)variant R258K | Streptomyces clavuligerus | ampicillin2-oxohexanoate; 2-oxohexanoate (2-OH) | 0.009 | 24 | 4 × 10⁻⁴ | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258K, ampicillin |
| deacetoxycephalosporin C synthase (DAOCS)variant R258K | Streptomyces clavuligerus | ampicillin2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP) | 0.013 | 15 | 9 × 10⁻⁴ | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258K, ampicillin |
| deacetoxycephalosporin C synthase (DAOCS)variant R258K | Streptomyces clavuligerus | penicillin G2-oxoglutarate; 2-oxoglutarate (2-OG) | 0.01 | 6.4 | 0.0016 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258K, penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant R258K | Streptomyces clavuligerus | penicillin G2-oxohexanoate; 2-oxohexanoate (2-OH) | 0.001 | 4.7 | 0.0021 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258K, penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant R258K | Streptomyces clavuligerus | penicillin G2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP) | 0.02 | 7 | 0.0029 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258K, penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant R258L | Streptomyces clavuligerus | ampicillin2-oxohexanoate; 2-oxohexanoate (2-OH) | 0.012 | 2.6 | 0.0046 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258L, ampicillin |
| deacetoxycephalosporin C synthase (DAOCS)variant R258L | Streptomyces clavuligerus | ampicillin2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP) | 0.0086 | 1.3 | 0.0066 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258L, ampicillin |
| deacetoxycephalosporin C synthase (DAOCS)variant R258L | Streptomyces clavuligerus | penicillin G2-oxohexanoate; 2-oxohexanoate (2-OH) | 0.019 | 1.7 | 0.011 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258L, penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant R258L | Streptomyces clavuligerus | penicillin G2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP) | 0.02 | 1.2 | 0.017 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258L, penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant R258Q | Streptomyces clavuligerus | ampicillin2-oxohexanoate; 2-oxohexanoate (2-OH) | 0.017 | 6.6 | 0.0026 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258Q, ampicillin |
| deacetoxycephalosporin C synthase (DAOCS)variant R258Q | Streptomyces clavuligerus | ampicillin2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP) | 0.014 | 3.3 | 0.0042 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258Q, ampicillin |
| deacetoxycephalosporin C synthase (DAOCS)variant R258Q | Streptomyces clavuligerus | penicillin G2-oxohexanoate; 2-oxohexanoate (2-OH) | 0.01 | 0.79 | 0.013 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258Q, penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant R258Q | Streptomyces clavuligerus | penicillin G2-oxo-4-methyl-pentanoate; 2-oxo-4-methyl-pentanoate (2-OMP) | 0.021 | 1.5 | 0.014 | pH 7.5 | PMID 12631288 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant R258Q, penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant V275I (YS5) | Streptomyces clavuligerus | penicillin G | 0.0502 | 1.68 | 0.03 | pH 7.5, 30 °C | PMID 12676714 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant V275I (YS5), penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant V275I (YS5) | Streptomyces clavuligerus | penicillin N | 0.252 | 0.012 | 20 | pH 7.5, 30 °C | PMID 12676714 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant V275I (YS5), penicillin N |
| deacetoxycephalosporin C synthase (DAOCS)variant V275I/I305M (YS67) | Streptomyces clavuligerus | penicillin G | 0.1458 | 0.25 | 0.583 | pH 7.5, 30 °C | PMID 12676714 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant V275I/I305M (YS67), penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant V275I/I305M (YS67) | Streptomyces clavuligerus | penicillin N | 0.316 | 0.013 | 24 | pH 7.5, 30 °C | PMID 12676714 | Open record: deacetoxycephalosporin C synthase (DAOCS) variant V275I/I305M (YS67), penicillin N |
| deacetoxycephalosporin C synthase (DAOCS, expandase) | Streptomyces clavuligerus | penicillin G | 0.0453 | 2.58 | 0.018 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase), penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase) | Streptomyces clavuligerus | penicillin N | 0.307 | 0.014 | 22 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase), penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant A106T | Streptomyces clavuligerus | penicillin G | 0.0453 | 1.77 | 0.026 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant A106T, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant A106T | Streptomyces clavuligerus | penicillin N | 0.277 | 0.013 | 21 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant A106T, penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant C155Y | Streptomyces clavuligerus | penicillin G | 0.0476 | 1.76 | 0.027 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant C155Y, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant C155Y | Streptomyces clavuligerus | penicillin N | 0.235 | 0.016 | 15 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant C155Y, penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant C155Y/Y184H/V275I/C281Y | Streptomyces clavuligerus | penicillin G | 0.1398 | 0.19 | 0.736 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant C155Y/Y184H/V275I/C281Y, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant C155Y/Y184H/V275I/C281Y | Streptomyces clavuligerus | penicillin N | 0.048 | 0.092 | 0.521 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant C155Y/Y184H/V275I/C281Y, penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant H244Q | Streptomyces clavuligerus | penicillin G | 0.0698 | 1.39 | 0.05 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant H244Q, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant H244Q | Streptomyces clavuligerus | penicillin N | 0.26 | 0.009 | 29 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant H244Q, penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant L277Q | Streptomyces clavuligerus | penicillin G | 0.1036 | 1.02 | 0.102 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant L277Q, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant L277Q | Streptomyces clavuligerus | penicillin N | 0.297 | 0.011 | 27 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant L277Q, penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant M188I | Streptomyces clavuligerus | penicillin G | 0.0507 | 1.96 | 0.026 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant M188I, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant M188I | Streptomyces clavuligerus | penicillin N | 0.277 | 0.011 | 25 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant M188I, penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant M188V | Streptomyces clavuligerus | penicillin G | 0.0456 | 0.76 | 0.06 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant M188V, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant M188V | Streptomyces clavuligerus | penicillin N | 0.084 | 0.047 | 2 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant M188V, penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant M73T | Streptomyces clavuligerus | penicillin G | 0.0627 | 0.74 | 0.085 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant M73T, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant M73T | Streptomyces clavuligerus | penicillin N | 0.239 | 0.006 | 40 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant M73T, penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant T91A | Streptomyces clavuligerus | penicillin G | 0.0522 | 1.36 | 0.038 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant T91A, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant T91A | Streptomyces clavuligerus | penicillin N | 0.247 | 0.009 | 27 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant T91A, penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant Y184H | Streptomyces clavuligerus | penicillin G | 0.0798 | 0.95 | 0.084 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant Y184H, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant Y184H | Streptomyces clavuligerus | penicillin N | 0.048 | 0.295 | 0.163 | PMID 16332884 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant Y184H, penicillin N | |
| deacetoxycephalosporin C synthase (ScDAOCS) | Streptomyces clavuligerus | ampicillin | 0.059 | 6.94 | 0.0086 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS), ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant C281F | Streptomyces clavuligerus | ampicillin | 0.042 | 1.35 | 0.0312 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281F, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant C281Y | Streptomyces clavuligerus | ampicillin | 0.044 | 1.02 | 0.0435 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281Y, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant C281Y/I305M | Streptomyces clavuligerus | ampicillin | 0.122 | 0.21 | 0.5699 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281Y/I305M, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant C281Y/N304A | Streptomyces clavuligerus | ampicillin | 0.052 | 0.38 | 0.137 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281Y/N304A, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant C281Y/N304K | Streptomyces clavuligerus | ampicillin | 0.046 | 0.13 | 0.3619 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281Y/N304K, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant C281Y/N304L | Streptomyces clavuligerus | ampicillin | 0.043 | 0.34 | 0.1254 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281Y/N304L, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant C281Y/N304M | Streptomyces clavuligerus | ampicillin | 0.047 | 0.8 | 0.0593 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281Y/N304M, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant C281Y/N304R | Streptomyces clavuligerus | ampicillin | 0.043 | 0.05 | 0.8652 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281Y/N304R, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant C281Y/R306L | Streptomyces clavuligerus | ampicillin | 0.037 | 0.15 | 0.2543 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281Y/R306L, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant C281Y/R307L | Streptomyces clavuligerus | ampicillin | 0.046 | 1.2 | 0.0383 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281Y/R307L, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant I305M | Streptomyces clavuligerus | ampicillin | 0.104 | 1 | 0.1041 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant I305M, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant N304A | Streptomyces clavuligerus | ampicillin | 0.04 | 1.09 | 0.0365 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304A, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant N304A/R306L | Streptomyces clavuligerus | ampicillin | 0.034 | 0.2 | 0.1708 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304A/R306L, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant N304K | Streptomyces clavuligerus | ampicillin | 0.038 | 0.54 | 0.0701 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304K, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant N304K/R306L | Streptomyces clavuligerus | ampicillin | 0.033 | 0.12 | 0.2851 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304K/R306L, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant N304L | Streptomyces clavuligerus | ampicillin | 0.041 | 1.65 | 0.0248 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304L, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant N304L/R306L | Streptomyces clavuligerus | ampicillin | 0.029 | 0.4 | 0.0711 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304L/R306L, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant N304M | Streptomyces clavuligerus | ampicillin | 0.054 | 5.12 | 0.0105 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304M, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant N304M/R306L | Streptomyces clavuligerus | ampicillin | 0.026 | 0.36 | 0.0723 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304M/R306L, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant N304R | Streptomyces clavuligerus | ampicillin | 0.052 | 0.26 | 0.1971 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304R, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant N304R/R306L | Streptomyces clavuligerus | ampicillin | 0.03 | 0.19 | 0.1601 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304R/R306L, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant R306L | Streptomyces clavuligerus | ampicillin | 0.05 | 1.01 | 0.0492 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant R306L, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant R307L | Streptomyces clavuligerus | ampicillin | 0.05 | 5.96 | 0.0084 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant R307L, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant V275I | Streptomyces clavuligerus | ampicillin | 0.044 | 3.41 | 0.0128 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275I, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant V275I/I305M | Streptomyces clavuligerus | ampicillin | 0.105 | 0.7 | 0.1513 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275I/I305M, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant V275I/N304A | Streptomyces clavuligerus | ampicillin | 0.042 | 0.68 | 0.0617 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275I/N304A, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant V275I/N304K | Streptomyces clavuligerus | ampicillin | 0.04 | 0.38 | 0.1078 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275I/N304K, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant V275I/N304L | Streptomyces clavuligerus | ampicillin | 0.043 | 1.04 | 0.041 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275I/N304L, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant V275I/N304M | Streptomyces clavuligerus | ampicillin | 0.048 | 3.6 | 0.0135 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275I/N304M, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant V275I/N304R | Streptomyces clavuligerus | ampicillin | 0.039 | 0.11 | 0.3669 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275I/N304R, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant V275I/R306L | Streptomyces clavuligerus | ampicillin | 0.037 | 0.51 | 0.0723 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275I/R306L, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant V275I/R307L | Streptomyces clavuligerus | ampicillin | 0.049 | 4.27 | 0.0114 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275I/R307L, ampicillin |
| deacetoxycephalosporin C synthase (ScDAOCS)variant V275L | Streptomyces clavuligerus | ampicillin | 0.032 | 4.26 | 0.0075 | 30 °C | PMID 18083859 | Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275L, ampicillin |
| deacetoxycephalosporin/deacetylcephalosporin C synthase (DAOC/DACS) | Cephalosporium acremonium | penicillin G | 0.073 | 6.04 | 0.012 | PMID 14734549 | Open record: deacetoxycephalosporin/deacetylcephalosporin C synthase (DAOC/DACS), penicillin G | |
| deacetoxycephalosporin/deacetylcephalosporin C synthase (DAOC/DACS)variant M306I | Cephalosporium acremonium | penicillin G | 0.016 | 4.79 | 0.0033 | PMID 14734549 | Open record: deacetoxycephalosporin/deacetylcephalosporin C synthase (DAOC/DACS) variant M306I, penicillin G | |
| deacetoxycephalosporin/deacetylcephalosporin C synthase (DAOC/DACS)variant M306I | Cephalosporium acremonium | penicillin GC-terminally truncated at 310 (Δ310) | 0.091 | 6.42 | 0.0142 | PMID 14734549 | Open record: deacetoxycephalosporin/deacetylcephalosporin C synthase (DAOC/DACS) variant M306I, penicillin G | |
| deacetoxycephalosporin/deacetylcephalosporin C synthase (DAOC/DACS)variant N305L | Cephalosporium acremonium | penicillin G | 0.079 | 2.41 | 0.0328 | PMID 14734549 | Open record: deacetoxycephalosporin/deacetylcephalosporin C synthase (DAOC/DACS) variant N305L, penicillin G | |
| deacetoxycephalosporin/deacetylcephalosporin C synthase (DAOC/DACS)variant N305L/M306I | Cephalosporium acremonium | penicillin G | 0.07 | 2 | 0.035 | PMID 14734549 | Open record: deacetoxycephalosporin/deacetylcephalosporin C synthase (DAOC/DACS) variant N305L/M306I, penicillin G |