Values CatLog read from this paper
Engineering Streptomyces clavuligerus deacetoxycephalosporin C synthase for optimal ring expansion activity toward penicillin G
PMID 12676714doi:10.1128/aem.69.4.2306-2312.2003Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (48 values) from this paper: deacetoxycephalosporin C synthase (DAOCS) from Streptomyces clavuligerus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| deacetoxycephalosporin C synthase (DAOCS) | Streptomyces clavuligerus | penicillin G | 0.0453 | 2.58 | 0.018 | pH 7.5, 30 °C | Open record: deacetoxycephalosporin C synthase (DAOCS), penicillin G |
| deacetoxycephalosporin C synthase (DAOCS) | Streptomyces clavuligerus | penicillin N | 0.307 | 0.014 | 22 | pH 7.5, 30 °C | Open record: deacetoxycephalosporin C synthase (DAOCS), penicillin N |
| deacetoxycephalosporin C synthase (DAOCS)variant C281Y (YS53) | Streptomyces clavuligerus | penicillin G | 0.0744 | 0.68 | 0.109 | pH 7.5, 30 °C | Open record: deacetoxycephalosporin C synthase (DAOCS) variant C281Y (YS53), penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant C281Y (YS53) | Streptomyces clavuligerus | penicillin N | 0.273 | 0.006 | 47 | pH 7.5, 30 °C | Open record: deacetoxycephalosporin C synthase (DAOCS) variant C281Y (YS53), penicillin N |
| deacetoxycephalosporin C synthase (DAOCS)variant G79E (YS59) | Streptomyces clavuligerus | penicillin G | 0.0315 | 0.75 | 0.042 | pH 7.5, 30 °C | Open record: deacetoxycephalosporin C synthase (DAOCS) variant G79E (YS59), penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant G79E (YS59) | Streptomyces clavuligerus | penicillin N | 0.178 | 0.009 | 20 | pH 7.5, 30 °C | Open record: deacetoxycephalosporin C synthase (DAOCS) variant G79E (YS59), penicillin N |
| deacetoxycephalosporin C synthase (DAOCS)variant I305L (YS8) | Streptomyces clavuligerus | penicillin G | 0.0759 | 0.66 | 0.115 | pH 7.5, 30 °C | Open record: deacetoxycephalosporin C synthase (DAOCS) variant I305L (YS8), penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant I305L (YS8) | Streptomyces clavuligerus | penicillin N | 0.284 | 0.006 | 44 | pH 7.5, 30 °C | Open record: deacetoxycephalosporin C synthase (DAOCS) variant I305L (YS8), penicillin N |
| deacetoxycephalosporin C synthase (DAOCS)variant I305M (YS11) | Streptomyces clavuligerus | penicillin G | 0.1452 | 0.75 | 0.194 | pH 7.5, 30 °C | Open record: deacetoxycephalosporin C synthase (DAOCS) variant I305M (YS11), penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant I305M (YS11) | Streptomyces clavuligerus | penicillin N | 0.31 | 0.012 | 26 | pH 7.5, 30 °C | Open record: deacetoxycephalosporin C synthase (DAOCS) variant I305M (YS11), penicillin N |
| deacetoxycephalosporin C synthase (DAOCS)variant N304K (YS12) | Streptomyces clavuligerus | penicillin G | 0.0564 | 0.22 | 0.256 | pH 7.5, 30 °C | Open record: deacetoxycephalosporin C synthase (DAOCS) variant N304K (YS12), penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant N304K (YS12) | Streptomyces clavuligerus | penicillin N | 0.366 | 0.004 | 92 | pH 7.5, 30 °C | Open record: deacetoxycephalosporin C synthase (DAOCS) variant N304K (YS12), penicillin N |
| deacetoxycephalosporin C synthase (DAOCS)variant V275I (YS5) | Streptomyces clavuligerus | penicillin G | 0.0502 | 1.68 | 0.03 | pH 7.5, 30 °C | Open record: deacetoxycephalosporin C synthase (DAOCS) variant V275I (YS5), penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant V275I (YS5) | Streptomyces clavuligerus | penicillin N | 0.252 | 0.012 | 20 | pH 7.5, 30 °C | Open record: deacetoxycephalosporin C synthase (DAOCS) variant V275I (YS5), penicillin N |
| deacetoxycephalosporin C synthase (DAOCS)variant V275I/I305M (YS67) | Streptomyces clavuligerus | penicillin G | 0.1458 | 0.25 | 0.583 | pH 7.5, 30 °C | Open record: deacetoxycephalosporin C synthase (DAOCS) variant V275I/I305M (YS67), penicillin G |
| deacetoxycephalosporin C synthase (DAOCS)variant V275I/I305M (YS67) | Streptomyces clavuligerus | penicillin N | 0.316 | 0.013 | 24 | pH 7.5, 30 °C | Open record: deacetoxycephalosporin C synthase (DAOCS) variant V275I/I305M (YS67), penicillin N |