Values CatLog read from this paper

Relevant double mutations in bioengineered Streptomyces clavuligerus deacetoxycephalosporin C synthase result in higher binding specificities which improve penicillin bioconversion

PMID 18083859doi:10.1128/aem.02230-07Open in Browse

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CatLog read 34 records (102 values) from this paper: deacetoxycephalosporin C synthase (ScDAOCS) from Streptomyces clavuligerus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.14.20.1

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
deacetoxycephalosporin C synthase (ScDAOCS)	Streptomyces clavuligerus	ampicillin	0.059	6.94	0.0086	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS), ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant C281F	Streptomyces clavuligerus	ampicillin	0.042	1.35	0.0312	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281F, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant C281Y	Streptomyces clavuligerus	ampicillin	0.044	1.02	0.0435	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281Y, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant C281Y/I305M	Streptomyces clavuligerus	ampicillin	0.122	0.21	0.5699	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281Y/I305M, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant C281Y/N304A	Streptomyces clavuligerus	ampicillin	0.052	0.38	0.137	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281Y/N304A, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant C281Y/N304K	Streptomyces clavuligerus	ampicillin	0.046	0.13	0.3619	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281Y/N304K, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant C281Y/N304L	Streptomyces clavuligerus	ampicillin	0.043	0.34	0.1254	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281Y/N304L, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant C281Y/N304M	Streptomyces clavuligerus	ampicillin	0.047	0.8	0.0593	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281Y/N304M, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant C281Y/N304R	Streptomyces clavuligerus	ampicillin	0.043	0.05	0.8652	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281Y/N304R, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant C281Y/R306L	Streptomyces clavuligerus	ampicillin	0.037	0.15	0.2543	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281Y/R306L, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant C281Y/R307L	Streptomyces clavuligerus	ampicillin	0.046	1.2	0.0383	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant C281Y/R307L, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant I305M	Streptomyces clavuligerus	ampicillin	0.104	1	0.1041	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant I305M, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant N304A	Streptomyces clavuligerus	ampicillin	0.04	1.09	0.0365	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304A, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant N304A/R306L	Streptomyces clavuligerus	ampicillin	0.034	0.2	0.1708	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304A/R306L, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant N304K	Streptomyces clavuligerus	ampicillin	0.038	0.54	0.0701	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304K, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant N304K/R306L	Streptomyces clavuligerus	ampicillin	0.033	0.12	0.2851	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304K/R306L, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant N304L	Streptomyces clavuligerus	ampicillin	0.041	1.65	0.0248	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304L, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant N304L/R306L	Streptomyces clavuligerus	ampicillin	0.029	0.4	0.0711	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304L/R306L, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant N304M	Streptomyces clavuligerus	ampicillin	0.054	5.12	0.0105	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304M, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant N304M/R306L	Streptomyces clavuligerus	ampicillin	0.026	0.36	0.0723	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304M/R306L, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant N304R	Streptomyces clavuligerus	ampicillin	0.052	0.26	0.1971	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304R, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant N304R/R306L	Streptomyces clavuligerus	ampicillin	0.03	0.19	0.1601	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant N304R/R306L, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant R306L	Streptomyces clavuligerus	ampicillin	0.05	1.01	0.0492	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant R306L, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant R307L	Streptomyces clavuligerus	ampicillin	0.05	5.96	0.0084	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant R307L, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant V275I	Streptomyces clavuligerus	ampicillin	0.044	3.41	0.0128	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275I, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant V275I/I305M	Streptomyces clavuligerus	ampicillin	0.105	0.7	0.1513	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275I/I305M, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant V275I/N304A	Streptomyces clavuligerus	ampicillin	0.042	0.68	0.0617	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275I/N304A, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant V275I/N304K	Streptomyces clavuligerus	ampicillin	0.04	0.38	0.1078	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275I/N304K, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant V275I/N304L	Streptomyces clavuligerus	ampicillin	0.043	1.04	0.041	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275I/N304L, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant V275I/N304M	Streptomyces clavuligerus	ampicillin	0.048	3.6	0.0135	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275I/N304M, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant V275I/N304R	Streptomyces clavuligerus	ampicillin	0.039	0.11	0.3669	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275I/N304R, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant V275I/R306L	Streptomyces clavuligerus	ampicillin	0.037	0.51	0.0723	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275I/R306L, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant V275I/R307L	Streptomyces clavuligerus	ampicillin	0.049	4.27	0.0114	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275I/R307L, ampicillin
deacetoxycephalosporin C synthase (ScDAOCS)variant V275L	Streptomyces clavuligerus	ampicillin	0.032	4.26	0.0075	30 °C	Open record: deacetoxycephalosporin C synthase (ScDAOCS) variant V275L, ampicillin