Values CatLog read from this paper
Directed evolution of Streptomyces clavuligerus deacetoxycephalosporin C synthase for enhancement of penicillin G expansion
PMID 16332884doi:10.1128/aem.71.12.8873-8880.2005Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 22 records (66 values) from this paper: deacetoxycephalosporin C synthase (DAOCS, expandase) from Streptomyces clavuligerus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| deacetoxycephalosporin C synthase (DAOCS, expandase) | Streptomyces clavuligerus | penicillin G | 0.0453 | 2.58 | 0.018 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase), penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase) | Streptomyces clavuligerus | penicillin N | 0.307 | 0.014 | 22 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase), penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant A106T | Streptomyces clavuligerus | penicillin G | 0.0453 | 1.77 | 0.026 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant A106T, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant A106T | Streptomyces clavuligerus | penicillin N | 0.277 | 0.013 | 21 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant A106T, penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant C155Y | Streptomyces clavuligerus | penicillin G | 0.0476 | 1.76 | 0.027 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant C155Y, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant C155Y | Streptomyces clavuligerus | penicillin N | 0.235 | 0.016 | 15 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant C155Y, penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant C155Y/Y184H/V275I/C281Y | Streptomyces clavuligerus | penicillin G | 0.1398 | 0.19 | 0.736 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant C155Y/Y184H/V275I/C281Y, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant C155Y/Y184H/V275I/C281Y | Streptomyces clavuligerus | penicillin N | 0.048 | 0.092 | 0.521 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant C155Y/Y184H/V275I/C281Y, penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant H244Q | Streptomyces clavuligerus | penicillin G | 0.0698 | 1.39 | 0.05 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant H244Q, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant H244Q | Streptomyces clavuligerus | penicillin N | 0.26 | 0.009 | 29 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant H244Q, penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant L277Q | Streptomyces clavuligerus | penicillin G | 0.1036 | 1.02 | 0.102 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant L277Q, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant L277Q | Streptomyces clavuligerus | penicillin N | 0.297 | 0.011 | 27 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant L277Q, penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant M188I | Streptomyces clavuligerus | penicillin G | 0.0507 | 1.96 | 0.026 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant M188I, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant M188I | Streptomyces clavuligerus | penicillin N | 0.277 | 0.011 | 25 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant M188I, penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant M188V | Streptomyces clavuligerus | penicillin G | 0.0456 | 0.76 | 0.06 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant M188V, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant M188V | Streptomyces clavuligerus | penicillin N | 0.084 | 0.047 | 2 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant M188V, penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant M73T | Streptomyces clavuligerus | penicillin G | 0.0627 | 0.74 | 0.085 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant M73T, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant M73T | Streptomyces clavuligerus | penicillin N | 0.239 | 0.006 | 40 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant M73T, penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant T91A | Streptomyces clavuligerus | penicillin G | 0.0522 | 1.36 | 0.038 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant T91A, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant T91A | Streptomyces clavuligerus | penicillin N | 0.247 | 0.009 | 27 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant T91A, penicillin N | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant Y184H | Streptomyces clavuligerus | penicillin G | 0.0798 | 0.95 | 0.084 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant Y184H, penicillin G | |
| deacetoxycephalosporin C synthase (DAOCS, expandase)variant Y184H | Streptomyces clavuligerus | penicillin N | 0.048 | 0.295 | 0.163 | Open record: deacetoxycephalosporin C synthase (DAOCS, expandase) variant Y184H, penicillin N |