Values CatLog read from this paper
How does Pseudomonas fluorescens avoid suicide from its antibiotic pseudomonic acid?: Evidence for two evolutionarily distinct isoleucyl-tRNA synthetases conferring self-defense
PMID 12672810doi:10.1074/jbc.m302633200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (12 values) from this paper: isoleucyl-tRNA synthetase IleRS-R2, isoleucyl-tRNA synthetase (crude extract activity: IleRS-R1 plus IleRS-R2), isoleucyl-tRNA synthetase IleRS-R1 from Pseudomonas fluorescens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 3 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| isoleucyl-tRNA synthetase (crude extract activity: IleRS-R1 plus IleRS-R2) | Pseudomonas fluorescens | adenosine 5'-triphosphate | — | 0.0152 | — | pH 7.5 | Open record: isoleucyl-tRNA synthetase (crude extract activity: IleRS-R1 plus IleRS-R2), adenosine 5'-triphosphate |
| isoleucyl-tRNA synthetase (crude extract activity: IleRS-R1 plus IleRS-R2) | Pseudomonas fluorescens | adenosine 5'-triphosphate | — | 0.1429 | — | pH 7.5 | Open record: isoleucyl-tRNA synthetase (crude extract activity: IleRS-R1 plus IleRS-R2), adenosine 5'-triphosphate |
| isoleucyl-tRNA synthetase (crude extract activity: IleRS-R1 plus IleRS-R2) | Pseudomonas fluorescens | L-isoleucine | — | 0.0104 | — | pH 7.5 | Open record: isoleucyl-tRNA synthetase (crude extract activity: IleRS-R1 plus IleRS-R2), L-isoleucine |
| isoleucyl-tRNA synthetase IleRS-R1 | Pseudomonas fluorescens | adenosine 5'-triphosphate | — | 0.00704 | — | pH 7.5 | Open record: isoleucyl-tRNA synthetase IleRS-R1, adenosine 5'-triphosphate |
| isoleucyl-tRNA synthetase IleRS-R1 | Pseudomonas fluorescens | L-isoleucine | 0.177 | 0.00125 | 142 calc. | pH 7.5 | Open record: isoleucyl-tRNA synthetase IleRS-R1, L-isoleucine |
| isoleucyl-tRNA synthetase IleRS-R2 | Pseudomonas fluorescens | adenosine 5'-triphosphaterecombinant | — | 0.285 | — | pH 7.5 | Open record: isoleucyl-tRNA synthetase IleRS-R2, adenosine 5'-triphosphate |
| isoleucyl-tRNA synthetase IleRS-R2 | Pseudomonas fluorescens | adenosine 5'-triphosphatenative; purified from P. fluorescens NCIB10586 | — | 0.33 | — | pH 7.5 | Open record: isoleucyl-tRNA synthetase IleRS-R2, adenosine 5'-triphosphate |
| isoleucyl-tRNA synthetase IleRS-R2 | Pseudomonas fluorescens | L-isoleucinerecombinant | 2.64 | 0.0122 | 216 calc. | pH 7.5 | Open record: isoleucyl-tRNA synthetase IleRS-R2, L-isoleucine |
| isoleucyl-tRNA synthetase IleRS-R2 | Pseudomonas fluorescens | L-isoleucinenative; purified from P. fluorescens NCIB10586 | 4.627 | 0.01 | 460 calc. | pH 7.5 | Open record: isoleucyl-tRNA synthetase IleRS-R2, L-isoleucine |