CatLogenzyme kinetics catalog

EC 6.1.1.5: isoleucine-tRNA ligase

LigasesOpen in Browse

CatLog read 33 records for EC 6.1.1.5 from 3 papers, for 3 organisms. Names in these papers: isoleucyl-tRNA synthetase (Lc-IleRS), isoleucyl-tRNA synthetase (IleRS), isoleucyl-tRNA synthetase IleRS-R2, isoleucyl-tRNA synthetase IleRS-R1.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 37 records for EC 6.1.1.5 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.1.1.5 in Browse.

Papers

PaperOrganismsRecords
Recognition of tRNAIle with a UAU anticodon by isoleucyl-tRNA synthetase in lactic acid bacteriaLactobacillus casei14
Determinants for tRNA-dependent pretransfer editing in the synthetic site of isoleucyl-tRNA synthetaseEscherichia coli13
How does Pseudomonas fluorescens avoid suicide from its antibiotic pseudomonic acid?: Evidence for two evolutionarily distinct isoleucyl-tRNA synthetases conferring self-defensePseudomonas fluorescens6

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
isoleucyl-tRNA synthetase (IleRS)Escherichia colitRNAIle0.86——pH 7.5, 37 °CPMID 25207837Open
isoleucyl-tRNA synthetase (IleRS)variant D342AEscherichia coliL-isoleucine470.00529.04 × 10³pH 7.5, 37 °CPMID 25207837Open
isoleucyl-tRNA synthetase (IleRS)variant D342AEscherichia coliL-valine330.5659pH 7.5, 37 °CPMID 25207837Open
isoleucyl-tRNA synthetase (IleRS)variant Y59FEscherichia coliL-isoleucine210.00268.1 × 10³pH 7.5, 37 °CPMID 25207837Open
isoleucyl-tRNA synthetase (IleRS)variant Y59FEscherichia coliL-valine160.280pH 7.5, 37 °CPMID 25207837Open
isoleucyl-tRNA synthetase (IleRS)variant Y59FEscherichia colitRNAIle0.024——pH 7.5, 37 °CPMID 25207837Open
isoleucyl-tRNA synthetase (IleRS)variant Y59F/D342AEscherichia coliL-isoleucine230.00191.21 × 10⁴pH 7.5, 37 °CPMID 25207837Open
isoleucyl-tRNA synthetase (IleRS)variant Y59F/D342AEscherichia coliL-valine150.275pH 7.5, 37 °CPMID 25207837Open
isoleucyl-tRNA synthetase (IleRS)variant Y59TEscherichia coliL-isoleucine11.40.0415270pH 7.5, 37 °CPMID 25207837Open
isoleucyl-tRNA synthetase (IleRS)variant Y59TEscherichia coliL-valine9.85.71.7pH 7.5, 37 °CPMID 25207837Open
isoleucyl-tRNA synthetase (IleRS)variant Y59TEscherichia colitRNAIle0.074——pH 7.5, 37 °CPMID 25207837Open
isoleucyl-tRNA synthetase (IleRS)variant Y59T/D342AEscherichia coliL-isoleucine10.70.0383280pH 7.5, 37 °CPMID 25207837Open
isoleucyl-tRNA synthetase (IleRS)variant Y59T/D342AEscherichia coliL-valine8.25.31.5pH 7.5, 37 °CPMID 25207837Open
isoleucyl-tRNA synthetase (Lc-IleRS)Lactobacillus caseiLactobacillus casei tRNAIle1 (anticodon GAU), in vitro T7 transcript0.00111.2 × 10⁻⁴9.2 calc.pH 7.5, 37 °CPMID 35122395Open
isoleucyl-tRNA synthetase (Lc-IleRS)Lactobacillus caseiLactobacillus casei tRNAIle2 (anticodon UAU), in vitro T7 transcript2.4 × 10⁻⁴3.7 × 10⁻⁴0.65 calc.pH 7.5, 37 °CPMID 35122395Open
isoleucyl-tRNA synthetase (Lc-IleRS)variant A18GLactobacillus caseiLactobacillus casei tRNAIle1 (anticodon GAU), in vitro T7 transcript1.5 × 10⁻⁴0.00160.094 calc.pH 7.5, 37 °CPMID 35122395Open
isoleucyl-tRNA synthetase (Lc-IleRS)variant A18G/G19NLactobacillus caseiLactobacillus casei tRNAIle1 (anticodon GAU), in vitro T7 transcript5.4 × 10⁻⁴5 × 10⁻⁵11 calc.pH 7.5, 37 °CPMID 35122395Open
isoleucyl-tRNA synthetase (Lc-IleRS)variant G19NLactobacillus caseiLactobacillus casei tRNAIle1 (anticodon GAU), in vitro T7 transcript0.00148.5 × 10⁻⁵16 calc.pH 7.5, 37 °CPMID 35122395Open
isoleucyl-tRNA synthetase (Lc-IleRS)variant G19NLactobacillus caseiLactobacillus casei tRNAIle2 (anticodon UAU), in vitro T7 transcript2.3 × 10⁻⁴6.2 × 10⁻⁵3.7 calc.pH 7.5, 37 °CPMID 35122395Open
isoleucyl-tRNA synthetase (Lc-IleRS)variant K22VLactobacillus caseiLactobacillus casei tRNAIle1 (anticodon GAU), in vitro T7 transcript9.5 × 10⁻⁴1.3 × 10⁻⁴7.3 calc.pH 7.5, 37 °CPMID 35122395Open
isoleucyl-tRNA synthetase (Lc-IleRS)variant K22VLactobacillus caseiLactobacillus casei tRNAIle2 (anticodon UAU), in vitro T7 transcript2.2 × 10⁻⁴2.7 × 10⁻⁴0.81 calc.pH 7.5, 37 °CPMID 35122395Open
isoleucyl-tRNA synthetase (Lc-IleRS)variant N23KLactobacillus caseiLactobacillus casei tRNAIle1 (anticodon GAU), in vitro T7 transcript0.00430.0014.3 calc.pH 7.5, 37 °CPMID 35122395Open
isoleucyl-tRNA synthetase (Lc-IleRS)variant N23KLactobacillus caseiLactobacillus casei tRNAIle2 (anticodon UAU), in vitro T7 transcript3.3 × 10⁻⁴2.8 × 10⁻⁴1.2 calc.pH 7.5, 37 °CPMID 35122395Open
isoleucyl-tRNA synthetase (Lc-IleRS)variant P15KLactobacillus caseiLactobacillus casei tRNAIle1 (anticodon GAU), in vitro T7 transcript0.00152.5 × 10⁻⁴6.0 calc.pH 7.5, 37 °CPMID 35122395Open
isoleucyl-tRNA synthetase (Lc-IleRS)variant P15KLactobacillus caseiLactobacillus casei tRNAIle2 (anticodon UAU), in vitro T7 transcript1.7 × 10⁻⁴1.2 × 10⁻⁴1.4 calc.pH 7.5, 37 °CPMID 35122395Open
isoleucyl-tRNA synthetase (Lc-IleRS)variant S13KLactobacillus caseiLactobacillus casei tRNAIle1 (anticodon GAU), in vitro T7 transcript0.00131.5 × 10⁻⁴8.7 calc.pH 7.5, 37 °CPMID 35122395Open
isoleucyl-tRNA synthetase (Lc-IleRS)variant S13KLactobacillus caseiLactobacillus casei tRNAIle2 (anticodon UAU), in vitro T7 transcript3.1 × 10⁻⁴2.6 × 10⁻⁴1.2 calc.pH 7.5, 37 °CPMID 35122395Open
isoleucyl-tRNA synthetase IleRS-R1Pseudomonas fluorescensadenosine 5'-triphosphate—0.00704—pH 7.5PMID 12672810Open
isoleucyl-tRNA synthetase IleRS-R1Pseudomonas fluorescensL-isoleucine0.1770.00125142 calc.pH 7.5PMID 12672810Open
isoleucyl-tRNA synthetase IleRS-R2Pseudomonas fluorescensadenosine 5'-triphosphaterecombinant—0.285—pH 7.5PMID 12672810Open
isoleucyl-tRNA synthetase IleRS-R2Pseudomonas fluorescensadenosine 5'-triphosphatenative; purified from P. fluorescens NCIB10586—0.33—pH 7.5PMID 12672810Open
isoleucyl-tRNA synthetase IleRS-R2Pseudomonas fluorescensL-isoleucinerecombinant2.640.0122216 calc.pH 7.5PMID 12672810Open
isoleucyl-tRNA synthetase IleRS-R2Pseudomonas fluorescensL-isoleucinenative; purified from P. fluorescens NCIB105864.6270.01460 calc.pH 7.5PMID 12672810Open