CatLogenzyme kinetics catalog

Values CatLog read from this paper

Recognition of tRNAIle with a UAU anticodon by isoleucyl-tRNA synthetase in lactic acid bacteria

PMID 35122395doi:10.1111/febs.16389Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 14 records (28 values) from this paper: isoleucyl-tRNA synthetase (Lc-IleRS) from Lactobacillus casei.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 14 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.1.1.5

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
isoleucyl-tRNA synthetase (Lc-IleRS)Lactobacillus caseiLactobacillus casei tRNAIle1 (anticodon GAU), in vitro T7 transcript0.00111.2 × 10⁻⁴9.2 calc.pH 7.5, 37 °COpen
isoleucyl-tRNA synthetase (Lc-IleRS)Lactobacillus caseiLactobacillus casei tRNAIle2 (anticodon UAU), in vitro T7 transcript2.4 × 10⁻⁴3.7 × 10⁻⁴0.65 calc.pH 7.5, 37 °COpen
isoleucyl-tRNA synthetase (Lc-IleRS)variant A18GLactobacillus caseiLactobacillus casei tRNAIle1 (anticodon GAU), in vitro T7 transcript1.5 × 10⁻⁴0.00160.094 calc.pH 7.5, 37 °COpen
isoleucyl-tRNA synthetase (Lc-IleRS)variant A18G/G19NLactobacillus caseiLactobacillus casei tRNAIle1 (anticodon GAU), in vitro T7 transcript5.4 × 10⁻⁴5 × 10⁻⁵11 calc.pH 7.5, 37 °COpen
isoleucyl-tRNA synthetase (Lc-IleRS)variant G19NLactobacillus caseiLactobacillus casei tRNAIle1 (anticodon GAU), in vitro T7 transcript0.00148.5 × 10⁻⁵16 calc.pH 7.5, 37 °COpen
isoleucyl-tRNA synthetase (Lc-IleRS)variant G19NLactobacillus caseiLactobacillus casei tRNAIle2 (anticodon UAU), in vitro T7 transcript2.3 × 10⁻⁴6.2 × 10⁻⁵3.7 calc.pH 7.5, 37 °COpen
isoleucyl-tRNA synthetase (Lc-IleRS)variant K22VLactobacillus caseiLactobacillus casei tRNAIle1 (anticodon GAU), in vitro T7 transcript9.5 × 10⁻⁴1.3 × 10⁻⁴7.3 calc.pH 7.5, 37 °COpen
isoleucyl-tRNA synthetase (Lc-IleRS)variant K22VLactobacillus caseiLactobacillus casei tRNAIle2 (anticodon UAU), in vitro T7 transcript2.2 × 10⁻⁴2.7 × 10⁻⁴0.81 calc.pH 7.5, 37 °COpen
isoleucyl-tRNA synthetase (Lc-IleRS)variant N23KLactobacillus caseiLactobacillus casei tRNAIle1 (anticodon GAU), in vitro T7 transcript0.00430.0014.3 calc.pH 7.5, 37 °COpen
isoleucyl-tRNA synthetase (Lc-IleRS)variant N23KLactobacillus caseiLactobacillus casei tRNAIle2 (anticodon UAU), in vitro T7 transcript3.3 × 10⁻⁴2.8 × 10⁻⁴1.2 calc.pH 7.5, 37 °COpen
isoleucyl-tRNA synthetase (Lc-IleRS)variant P15KLactobacillus caseiLactobacillus casei tRNAIle1 (anticodon GAU), in vitro T7 transcript0.00152.5 × 10⁻⁴6.0 calc.pH 7.5, 37 °COpen
isoleucyl-tRNA synthetase (Lc-IleRS)variant P15KLactobacillus caseiLactobacillus casei tRNAIle2 (anticodon UAU), in vitro T7 transcript1.7 × 10⁻⁴1.2 × 10⁻⁴1.4 calc.pH 7.5, 37 °COpen
isoleucyl-tRNA synthetase (Lc-IleRS)variant S13KLactobacillus caseiLactobacillus casei tRNAIle1 (anticodon GAU), in vitro T7 transcript0.00131.5 × 10⁻⁴8.7 calc.pH 7.5, 37 °COpen
isoleucyl-tRNA synthetase (Lc-IleRS)variant S13KLactobacillus caseiLactobacillus casei tRNAIle2 (anticodon UAU), in vitro T7 transcript3.1 × 10⁻⁴2.6 × 10⁻⁴1.2 calc.pH 7.5, 37 °COpen