Values CatLog read from this paper
Determinants for tRNA-dependent pretransfer editing in the synthetic site of isoleucyl-tRNA synthetase
PMID 25207837doi:10.1021/bi5007699Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (33 values) from this paper: isoleucyl-tRNA synthetase (IleRS) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| isoleucyl-tRNA synthetase (IleRS) | Escherichia coli | tRNAIle | 0.86 | — | — | pH 7.5, 37 °C | Open record: isoleucyl-tRNA synthetase (IleRS), tRNAIle |
| isoleucyl-tRNA synthetase (IleRS)variant D342A | Escherichia coli | L-isoleucine | 47 | 0.0052 | 9.04 × 10³ | pH 7.5, 37 °C | Open record: isoleucyl-tRNA synthetase (IleRS) variant D342A, L-isoleucine |
| isoleucyl-tRNA synthetase (IleRS)variant D342A | Escherichia coli | L-valine | 33 | 0.56 | 59 | pH 7.5, 37 °C | Open record: isoleucyl-tRNA synthetase (IleRS) variant D342A, L-valine |
| isoleucyl-tRNA synthetase (IleRS)variant Y59F | Escherichia coli | L-isoleucine | 21 | 0.0026 | 8.1 × 10³ | pH 7.5, 37 °C | Open record: isoleucyl-tRNA synthetase (IleRS) variant Y59F, L-isoleucine |
| isoleucyl-tRNA synthetase (IleRS)variant Y59F | Escherichia coli | L-valine | 16 | 0.2 | 80 | pH 7.5, 37 °C | Open record: isoleucyl-tRNA synthetase (IleRS) variant Y59F, L-valine |
| isoleucyl-tRNA synthetase (IleRS)variant Y59F | Escherichia coli | tRNAIle | 0.024 | — | — | pH 7.5, 37 °C | Open record: isoleucyl-tRNA synthetase (IleRS) variant Y59F, tRNAIle |
| isoleucyl-tRNA synthetase (IleRS)variant Y59F/D342A | Escherichia coli | L-isoleucine | 23 | 0.0019 | 1.21 × 10⁴ | pH 7.5, 37 °C | Open record: isoleucyl-tRNA synthetase (IleRS) variant Y59F/D342A, L-isoleucine |
| isoleucyl-tRNA synthetase (IleRS)variant Y59F/D342A | Escherichia coli | L-valine | 15 | 0.2 | 75 | pH 7.5, 37 °C | Open record: isoleucyl-tRNA synthetase (IleRS) variant Y59F/D342A, L-valine |
| isoleucyl-tRNA synthetase (IleRS)variant Y59T | Escherichia coli | L-isoleucine | 11.4 | 0.0415 | 270 | pH 7.5, 37 °C | Open record: isoleucyl-tRNA synthetase (IleRS) variant Y59T, L-isoleucine |
| isoleucyl-tRNA synthetase (IleRS)variant Y59T | Escherichia coli | L-valine | 9.8 | 5.7 | 1.7 | pH 7.5, 37 °C | Open record: isoleucyl-tRNA synthetase (IleRS) variant Y59T, L-valine |
| isoleucyl-tRNA synthetase (IleRS)variant Y59T | Escherichia coli | tRNAIle | 0.074 | — | — | pH 7.5, 37 °C | Open record: isoleucyl-tRNA synthetase (IleRS) variant Y59T, tRNAIle |
| isoleucyl-tRNA synthetase (IleRS)variant Y59T/D342A | Escherichia coli | L-isoleucine | 10.7 | 0.0383 | 280 | pH 7.5, 37 °C | Open record: isoleucyl-tRNA synthetase (IleRS) variant Y59T/D342A, L-isoleucine |
| isoleucyl-tRNA synthetase (IleRS)variant Y59T/D342A | Escherichia coli | L-valine | 8.2 | 5.3 | 1.5 | pH 7.5, 37 °C | Open record: isoleucyl-tRNA synthetase (IleRS) variant Y59T/D342A, L-valine |