CatLogenzyme kinetics catalog

Values CatLog read from this paper

Substitution of cysteine for selenocysteine in the catalytic center of type III iodothyronine deiodinase reduces catalytic efficiency and alters substrate preference

PMID 12746313doi:10.1210/en.2003-0084Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 19 records (19 values) from this paper: type III iodothyronine deiodinase (D3) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.21.99.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
type III iodothyronine deiodinase (D3)Homo sapiens3,3',5-triiodothyronine0.3 mM DTT; recombinant human D3 expressed in COS-1 cells—3.1 × 10⁻⁶—pH 7.2, 37 °COpen
type III iodothyronine deiodinase (D3)Homo sapiens3,3',5-triiodothyronine1 mM DTT; recombinant human D3 expressed in COS-1 cells—3.6 × 10⁻⁶—pH 7.2, 37 °COpen
type III iodothyronine deiodinase (D3)Homo sapiens3,3',5-triiodothyronine3 mM DTT; recombinant human D3 expressed in COS-1 cells—4.8 × 10⁻⁶—pH 7.2, 37 °COpen
type III iodothyronine deiodinase (D3)Homo sapiens3,3',5-triiodothyronine10 mM DTT; recombinant human D3 expressed in COS-1 cells—6.6 × 10⁻⁶—pH 7.2, 37 °COpen
type III iodothyronine deiodinase (D3)Homo sapiens3,3',5-triiodothyronine1 mM DTT; native D3 in human placenta microsomes—2.8 × 10⁻⁶—pH 7.2, 37 °COpen
type III iodothyronine deiodinase (D3)Homo sapiens3,3',5-triiodothyronine3 mM DTT; native D3 in human placenta microsomes—3.4 × 10⁻⁶—pH 7.2, 37 °COpen
type III iodothyronine deiodinase (D3)Homo sapiens3,3',5-triiodothyronine10 mM DTT; native D3 in human placenta microsomes—4.1 × 10⁻⁶—pH 7.2, 37 °COpen
type III iodothyronine deiodinase (D3)Homo sapiensT40.3 mM DTT; recombinant human D3 expressed in COS-1 cells—4.3 × 10⁻⁶—pH 7.2, 37 °COpen
type III iodothyronine deiodinase (D3)Homo sapiensT41 mM DTT; recombinant human D3 expressed in COS-1 cells—5.6 × 10⁻⁶—pH 7.2, 37 °COpen
type III iodothyronine deiodinase (D3)Homo sapiensT43 mM DTT; recombinant human D3 expressed in COS-1 cells—3.5 × 10⁻⁶—pH 7.2, 37 °COpen
type III iodothyronine deiodinase (D3)Homo sapiensT410 mM DTT; recombinant human D3 expressed in COS-1 cells—4.1 × 10⁻⁶—pH 7.2, 37 °COpen
type III iodothyronine deiodinase (D3)Homo sapiensT410 mM DTT; native D3 in human placenta microsomes—8 × 10⁻⁶—pH 7.2, 37 °COpen
type III iodothyronine deiodinase (D3)variant SeC144CysHomo sapiens3,3',5-triiodothyronine0.3 mM DTT—4.2 × 10⁻⁶—pH 7.2, 37 °COpen
type III iodothyronine deiodinase (D3)variant SeC144CysHomo sapiens3,3',5-triiodothyronine1 mM DTT—1.5 × 10⁻⁵—pH 7.2, 37 °COpen
type III iodothyronine deiodinase (D3)variant SeC144CysHomo sapiens3,3',5-triiodothyronine3 mM DTT—3.1 × 10⁻⁵—pH 7.2, 37 °COpen
type III iodothyronine deiodinase (D3)variant SeC144CysHomo sapiens3,3',5-triiodothyronine10 mM DTT—3.3 × 10⁻⁵—pH 7.2, 37 °COpen
type III iodothyronine deiodinase (D3)variant SeC144CysHomo sapiensT41 mM DTT—2.65 × 10⁻⁴—pH 7.2, 37 °COpen
type III iodothyronine deiodinase (D3)variant SeC144CysHomo sapiensT43 mM DTT—4.77 × 10⁻⁴—pH 7.2, 37 °COpen
type III iodothyronine deiodinase (D3)variant SeC144CysHomo sapiensT410 mM DTT—5.7 × 10⁻⁴—pH 7.2, 37 °COpen