Values CatLog read from this paper
Kinetic and spectroscopic characterization of the H178A methionyl aminopeptidase from Escherichia coli
PMID 12755633doi:10.1021/bi027327sOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (24 values) from this paper: methionyl aminopeptidase (EcMetAP-I) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| methionyl aminopeptidase (EcMetAP-I) | Escherichia coli | Met-Gly-Met-MetCo(II)-loaded enzyme; 12 µM CoCl2 | 39 | 3.2 | 12.2 | pH 7.5 | Open record: methionyl aminopeptidase (EcMetAP-I), Met-Gly-Met-Met |
| methionyl aminopeptidase (EcMetAP-I) | Escherichia coli | Met-Gly-Met-MetFe(II)-loaded enzyme; 12 µM Fe(SO4) | 28 | 2 | 14 | pH 7.5 | Open record: methionyl aminopeptidase (EcMetAP-I), Met-Gly-Met-Met |
| methionyl aminopeptidase (EcMetAP-I) | Escherichia coli | Met-Pro-p-nitroanilideCo(II)-loaded enzyme; 12 µM CoCl2 | 0.59 | 0.06 | 9.8 | pH 7.5, 30 °C | Open record: methionyl aminopeptidase (EcMetAP-I), Met-Pro-p-nitroanilide |
| methionyl aminopeptidase (EcMetAP-I) | Escherichia coli | Met-Pro-p-nitroanilideFe(II)-loaded enzyme; 12 µM Fe(SO4) | 1.4 | 0.09 | 16.8 | pH 7.5, 30 °C | Open record: methionyl aminopeptidase (EcMetAP-I), Met-Pro-p-nitroanilide |
| methionyl aminopeptidase (EcMetAP-I)variant H178A | Escherichia coli | Met-Gly-Met-MetCo(II)-loaded enzyme; 12 µM CoCl2 | 0.54 | 3.2 | 0.17 | pH 7.5 | Open record: methionyl aminopeptidase (EcMetAP-I) variant H178A, Met-Gly-Met-Met |
| methionyl aminopeptidase (EcMetAP-I)variant H178A | Escherichia coli | Met-Gly-Met-MetFe(II)-loaded enzyme; 12 µM Fe(SO4) | 0.56 | 2 | 0.28 | pH 7.5 | Open record: methionyl aminopeptidase (EcMetAP-I) variant H178A, Met-Gly-Met-Met |
| methionyl aminopeptidase (EcMetAP-I)variant H178A | Escherichia coli | Met-Pro-p-nitroanilideCo(II)-loaded enzyme; 12 µM CoCl2 | 0.002 | 0.12 | 0.015 | pH 7.5, 30 °C | Open record: methionyl aminopeptidase (EcMetAP-I) variant H178A, Met-Pro-p-nitroanilide |
| methionyl aminopeptidase (EcMetAP-I)variant H178A | Escherichia coli | Met-Pro-p-nitroanilideFe(II)-loaded enzyme; 12 µM Fe(SO4) | 0.01 | 0.13 | 0.085 | pH 7.5, 30 °C | Open record: methionyl aminopeptidase (EcMetAP-I) variant H178A, Met-Pro-p-nitroanilide |