Values CatLog read from this paper
Conformational dynamics of the active site loop of S-adenosylmethionine synthetase illuminated by site-directed spin labeling
PMID 12831838doi:10.1016/s0003-9861(03)00277-7Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (15 values) from this paper: S-adenosylmethionine synthetase (MAT) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| S-adenosylmethionine synthetase (MAT) | Escherichia coli | L-methionine | 1.5 | 0.11 | 14 calc. | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (MAT), L-methionine |
| S-adenosylmethionine synthetase (MAT) | Escherichia coli | magnesium adenosine 5'-triphosphate | — | 0.083 | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (MAT), magnesium adenosine 5'-triphosphate |
| S-adenosylmethionine synthetase (MAT)variant D107C | Escherichia coli | L-methionine | 1.6 | 0.29 | 5.5 calc. | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (MAT) variant D107C, L-methionine |
| S-adenosylmethionine synthetase (MAT)variant D107C | Escherichia coli | L-methionineD107C labeled at Cys107 with MTSL spin label (R1 side chain) | 0.23 | 0.19 | 1.2 calc. | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (MAT) variant D107C, L-methionine |
| S-adenosylmethionine synthetase (MAT)variant D107C | Escherichia coli | magnesium adenosine 5'-triphosphate | — | 0.13 | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (MAT) variant D107C, magnesium adenosine 5'-triphosphate |
| S-adenosylmethionine synthetase (MAT)variant D107C | Escherichia coli | magnesium adenosine 5'-triphosphateD107C labeled at Cys107 with MTSL spin label (R1 side chain) | — | 0.18 | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (MAT) variant D107C, magnesium adenosine 5'-triphosphate |
| S-adenosylmethionine synthetase (MAT)variant G105C | Escherichia coli | L-methionine | 1.6 | 0.45 | 3.6 calc. | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (MAT) variant G105C, L-methionine |
| S-adenosylmethionine synthetase (MAT)variant G105C | Escherichia coli | L-methionineG105C labeled at Cys105 with MTSL spin label (R1 side chain) | 1.4 | 0.75 | 1.9 calc. | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (MAT) variant G105C, L-methionine |
| S-adenosylmethionine synthetase (MAT)variant G105C | Escherichia coli | magnesium adenosine 5'-triphosphate | — | 0.13 | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (MAT) variant G105C, magnesium adenosine 5'-triphosphate |
| S-adenosylmethionine synthetase (MAT)variant G105C | Escherichia coli | magnesium adenosine 5'-triphosphateG105C labeled at Cys105 with MTSL spin label (R1 side chain) | — | 0.18 | — | pH 8, 22 °C | Open record: S-adenosylmethionine synthetase (MAT) variant G105C, magnesium adenosine 5'-triphosphate |