Values CatLog read from this paper

Alanine-scanning mutagenesis of the predicted rRNA-binding domain of ErmC' redefines the substrate-binding site and suggests a model for protein-RNA interactions

PMID 12907737doi:10.1093/nar/gkg666Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 32 records (64 values) from this paper: ErmC' rRNA adenine-N6 methyltransferase from Bacillus subtilis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 32 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.1.1.184

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
ErmC' rRNA adenine-N6 methyltransferase	Bacillus subtilis	32mer RNA oligonucleotide	0.00637	6.9 × 10⁻⁴	9.2 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferase	Bacillus subtilis	S-adenosyl-L-methionine	0.0655	0.00282	23.2 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant E202A	Bacillus subtilis	32mer RNA oligonucleotide	0.00211	5.7 × 10⁻⁴	3.7 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant E202A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant E202A	Bacillus subtilis	S-adenosyl-L-methionine	0.0735	0.00977	7.52 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant E202A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant K133A	Bacillus subtilis	32mer RNA oligonucleotide	0.00222	0.00186	1.19 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K133A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant K133A	Bacillus subtilis	S-adenosyl-L-methionine	0.038	0.006	6.3 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K133A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant K197A	Bacillus subtilis	32mer RNA oligonucleotide	0.00731	0.00159	4.60 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K197A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant K197A	Bacillus subtilis	S-adenosyl-L-methionine	0.0522	0.00334	15.6 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K197A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant K197A/N200A/E202A/K204A/K205A	Bacillus subtilis	32mer RNA oligonucleotide	0.00193	0.00121	1.60 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K197A/N200A/E202A/K204A/K205A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant K197A/N200A/E202A/K204A/K205A	Bacillus subtilis	S-adenosyl-L-methionine	0.0314	0.00656	4.79 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K197A/N200A/E202A/K204A/K205A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant K204A	Bacillus subtilis	32mer RNA oligonucleotide	0.00729	8.9 × 10⁻⁴	8.2 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K204A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant K204A	Bacillus subtilis	S-adenosyl-L-methionine	0.0648	0.00383	16.9 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K204A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant K205A	Bacillus subtilis	32mer RNA oligonucleotide	0.00766	9.1 × 10⁻⁴	8.4 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K205A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant K205A	Bacillus subtilis	S-adenosyl-L-methionine	0.0649	0.00443	14.7 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K205A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant K209A	Bacillus subtilis	32mer RNA oligonucleotide	0.00637	9.5 × 10⁻⁴	6.7 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K209A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant K209A	Bacillus subtilis	S-adenosyl-L-methionine	0.0775	0.00462	16.8 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K209A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant M196A	Bacillus subtilis	32mer RNA oligonucleotide	0.00318	6.2 × 10⁻⁴	5.1 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant M196A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant M196A	Bacillus subtilis	S-adenosyl-L-methionine	0.0318	0.00328	9.70 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant M196A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant N192A	Bacillus subtilis	32mer RNA oligonucleotide	0.00544	7.9 × 10⁻⁴	6.9 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant N192A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant N192A	Bacillus subtilis	S-adenosyl-L-methionine	0.0341	0.002	17 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant N192A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant N200A	Bacillus subtilis	32mer RNA oligonucleotide	0.00439	8.9 × 10⁻⁴	4.9 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant N200A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant N200A	Bacillus subtilis	S-adenosyl-L-methionine	0.0688	0.00709	9.70 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant N200A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant R112A	Bacillus subtilis	32mer RNA oligonucleotide	0.00113	0.0017	0.67 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant R112A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant R112A	Bacillus subtilis	S-adenosyl-L-methionine	0.0161	0.0058	2.8 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant R112A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant R112D	Bacillus subtilis	32mer RNA oligonucleotide	4.9 × 10⁻⁴	0.00364	0.13 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant R112D, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant R112D	Bacillus subtilis	S-adenosyl-L-methionine	0.0121	0.01076	1.12 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant R112D, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant R134A	Bacillus subtilis	32mer RNA oligonucleotide	2.1 × 10⁻⁴	0.0025	0.084 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant R134A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant R134A	Bacillus subtilis	S-adenosyl-L-methionine	0.0157	0.02132	0.736 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant R134A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant R140A	Bacillus subtilis	32mer RNA oligonucleotide	3.4 × 10⁻⁴	0.00141	0.24 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant R140A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant R140A	Bacillus subtilis	S-adenosyl-L-methionine	0.0149	0.02228	0.669 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant R140A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant T108A	Bacillus subtilis	32mer RNA oligonucleotide	1.1 × 10⁻⁴	4.3 × 10⁻⁴	0.26 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant T108A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant T108A	Bacillus subtilis	S-adenosyl-L-methionine	0.0229	0.02567	0.892 calc.	pH 7.5, 25 °C	Open record: ErmC' rRNA adenine-N6 methyltransferase variant T108A, S-adenosyl-L-methionine