EC 2.1.1.184: 23S rRNA (adenine2085-N6)-dimethyltransferase

TransferasesOpen in Browse

CatLog read 54 records for EC 2.1.1.184 from 3 papers, for 2 organisms. Names in these papers: ErmC' rRNA adenine-N6 methyltransferase, rRNA:m6A methyltransferase ErmC′, SaErmC.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 2 records for EC 2.1.1.184 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.1.1.184 in Browse.

Papers

Papers with records for EC 2.1.1.184
Paper	Organisms	Records
Alanine-scanning mutagenesis of the predicted rRNA-binding domain of ErmC' redefines the substrate-binding site and suggests a model for protein-RNA interactions	Bacillus subtilis	32
Mutational analysis defines the roles of conserved amino acid residues in the predicted catalytic pocket of the rRNA:m6A methyltransferase ErmC'	Bacillus subtilis	16
Antibiotic-Adjuvants Abolish Resistance Conferred by the Staphylococcus aureus Erythromycin Resistance Methyltransferase in an Escherichia coli Model	Staphylococcus aureus	6

Records

Records for EC 2.1.1.184 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
ErmC' rRNA adenine-N6 methyltransferase	Bacillus subtilis	32mer RNA oligonucleotide	0.00637	6.9 × 10⁻⁴	9.2 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferase	Bacillus subtilis	S-adenosyl-L-methionine	0.0655	0.00282	23.2 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant E202A	Bacillus subtilis	32mer RNA oligonucleotide	0.00211	5.7 × 10⁻⁴	3.7 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant E202A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant E202A	Bacillus subtilis	S-adenosyl-L-methionine	0.0735	0.00977	7.52 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant E202A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant K133A	Bacillus subtilis	32mer RNA oligonucleotide	0.00222	0.00186	1.19 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K133A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant K133A	Bacillus subtilis	S-adenosyl-L-methionine	0.038	0.006	6.3 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K133A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant K197A	Bacillus subtilis	32mer RNA oligonucleotide	0.00731	0.00159	4.60 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K197A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant K197A	Bacillus subtilis	S-adenosyl-L-methionine	0.0522	0.00334	15.6 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K197A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant K197A/N200A/E202A/K204A/K205A	Bacillus subtilis	32mer RNA oligonucleotide	0.00193	0.00121	1.60 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K197A/N200A/E202A/K204A/K205A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant K197A/N200A/E202A/K204A/K205A	Bacillus subtilis	S-adenosyl-L-methionine	0.0314	0.00656	4.79 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K197A/N200A/E202A/K204A/K205A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant K204A	Bacillus subtilis	32mer RNA oligonucleotide	0.00729	8.9 × 10⁻⁴	8.2 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K204A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant K204A	Bacillus subtilis	S-adenosyl-L-methionine	0.0648	0.00383	16.9 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K204A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant K205A	Bacillus subtilis	32mer RNA oligonucleotide	0.00766	9.1 × 10⁻⁴	8.4 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K205A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant K205A	Bacillus subtilis	S-adenosyl-L-methionine	0.0649	0.00443	14.7 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K205A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant K209A	Bacillus subtilis	32mer RNA oligonucleotide	0.00637	9.5 × 10⁻⁴	6.7 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K209A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant K209A	Bacillus subtilis	S-adenosyl-L-methionine	0.0775	0.00462	16.8 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant K209A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant M196A	Bacillus subtilis	32mer RNA oligonucleotide	0.00318	6.2 × 10⁻⁴	5.1 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant M196A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant M196A	Bacillus subtilis	S-adenosyl-L-methionine	0.0318	0.00328	9.70 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant M196A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant N192A	Bacillus subtilis	32mer RNA oligonucleotide	0.00544	7.9 × 10⁻⁴	6.9 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant N192A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant N192A	Bacillus subtilis	S-adenosyl-L-methionine	0.0341	0.002	17 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant N192A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant N200A	Bacillus subtilis	32mer RNA oligonucleotide	0.00439	8.9 × 10⁻⁴	4.9 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant N200A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant N200A	Bacillus subtilis	S-adenosyl-L-methionine	0.0688	0.00709	9.70 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant N200A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant R112A	Bacillus subtilis	32mer RNA oligonucleotide	0.00113	0.0017	0.67 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant R112A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant R112A	Bacillus subtilis	S-adenosyl-L-methionine	0.0161	0.0058	2.8 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant R112A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant R112D	Bacillus subtilis	32mer RNA oligonucleotide	4.9 × 10⁻⁴	0.00364	0.13 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant R112D, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant R112D	Bacillus subtilis	S-adenosyl-L-methionine	0.0121	0.01076	1.12 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant R112D, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant R134A	Bacillus subtilis	32mer RNA oligonucleotide	2.1 × 10⁻⁴	0.0025	0.084 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant R134A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant R134A	Bacillus subtilis	S-adenosyl-L-methionine	0.0157	0.02132	0.736 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant R134A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant R140A	Bacillus subtilis	32mer RNA oligonucleotide	3.4 × 10⁻⁴	0.00141	0.24 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant R140A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant R140A	Bacillus subtilis	S-adenosyl-L-methionine	0.0149	0.02228	0.669 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant R140A, S-adenosyl-L-methionine
ErmC' rRNA adenine-N6 methyltransferasevariant T108A	Bacillus subtilis	32mer RNA oligonucleotide	1.1 × 10⁻⁴	4.3 × 10⁻⁴	0.26 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant T108A, 32mer RNA oligonucleotide
ErmC' rRNA adenine-N6 methyltransferasevariant T108A	Bacillus subtilis	S-adenosyl-L-methionine	0.0229	0.02567	0.892 calc.	pH 7.5, 25 °C	PMID 12907737	Open record: ErmC' rRNA adenine-N6 methyltransferase variant T108A, S-adenosyl-L-methionine
rRNA:m6A methyltransferase ErmC′	Bacillus subtilis	32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA	0.0064	7 × 10⁻⁴	9.1 calc.	pH 7.5, 25 °C	PMID 12946350	Open record: rRNA:m6A methyltransferase ErmC′, 32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA
rRNA:m6A methyltransferase ErmC′	Bacillus subtilis	S-adenosyl-L-methionine	0.066	0.0028	24 calc.	pH 7.5, 25 °C	PMID 12946350	Open record: rRNA:m6A methyltransferase ErmC′, S-adenosyl-L-methionine
rRNA:m6A methyltransferase ErmC′variant E128A	Bacillus subtilis	32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA	0.0019	3 × 10⁻⁴	6.3 calc.	pH 7.5, 25 °C	PMID 12946350	Open record: rRNA:m6A methyltransferase ErmC′ variant E128A, 32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA
rRNA:m6A methyltransferase ErmC′variant E128A	Bacillus subtilis	S-adenosyl-L-methionine	0.048	0.0045	11 calc.	pH 7.5, 25 °C	PMID 12946350	Open record: rRNA:m6A methyltransferase ErmC′ variant E128A, S-adenosyl-L-methionine
rRNA:m6A methyltransferase ErmC′variant F163A	Bacillus subtilis	32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA	0.0037	0.0014	2.6 calc.	pH 7.5, 25 °C	PMID 12946350	Open record: rRNA:m6A methyltransferase ErmC′ variant F163A, 32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA
rRNA:m6A methyltransferase ErmC′variant F163A	Bacillus subtilis	S-adenosyl-L-methionine	0.032	0.002	16 calc.	pH 7.5, 25 °C	PMID 12946350	Open record: rRNA:m6A methyltransferase ErmC′ variant F163A, S-adenosyl-L-methionine
rRNA:m6A methyltransferase ErmC′variant K166A	Bacillus subtilis	32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA	0.022	0.0041	5.4 calc.	pH 7.5, 25 °C	PMID 12946350	Open record: rRNA:m6A methyltransferase ErmC′ variant K166A, 32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA
rRNA:m6A methyltransferase ErmC′variant K166A	Bacillus subtilis	S-adenosyl-L-methionine	0.054	0.0031	17 calc.	pH 7.5, 25 °C	PMID 12946350	Open record: rRNA:m6A methyltransferase ErmC′ variant K166A, S-adenosyl-L-methionine
rRNA:m6A methyltransferase ErmC′variant K168A	Bacillus subtilis	32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA	0.0087	0.0014	6.2 calc.	pH 7.5, 25 °C	PMID 12946350	Open record: rRNA:m6A methyltransferase ErmC′ variant K168A, 32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA
rRNA:m6A methyltransferase ErmC′variant K168A	Bacillus subtilis	S-adenosyl-L-methionine	0.054	0.004	14 calc.	pH 7.5, 25 °C	PMID 12946350	Open record: rRNA:m6A methyltransferase ErmC′ variant K168A, S-adenosyl-L-methionine
rRNA:m6A methyltransferase ErmC′variant N101A	Bacillus subtilis	32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA	7 × 10⁻⁴	8 × 10⁻⁴	0.88 calc.	pH 7.5, 25 °C	PMID 12946350	Open record: rRNA:m6A methyltransferase ErmC′ variant N101A, 32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA
rRNA:m6A methyltransferase ErmC′variant N101A	Bacillus subtilis	S-adenosyl-L-methionine	0.033	0.0139	2.4 calc.	pH 7.5, 25 °C	PMID 12946350	Open record: rRNA:m6A methyltransferase ErmC′ variant N101A, S-adenosyl-L-methionine
rRNA:m6A methyltransferase ErmC′variant N11A	Bacillus subtilis	32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA	0.0023	8 × 10⁻⁴	2.9 calc.	pH 7.5, 25 °C	PMID 12946350	Open record: rRNA:m6A methyltransferase ErmC′ variant N11A, 32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA
rRNA:m6A methyltransferase ErmC′variant N11A	Bacillus subtilis	S-adenosyl-L-methionine	0.029	0.004	7.2 calc.	pH 7.5, 25 °C	PMID 12946350	Open record: rRNA:m6A methyltransferase ErmC′ variant N11A, S-adenosyl-L-methionine
rRNA:m6A methyltransferase ErmC′variant P165A	Bacillus subtilis	32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA	4 × 10⁻⁴	0.0013	0.31 calc.	pH 7.5, 25 °C	PMID 12946350	Open record: rRNA:m6A methyltransferase ErmC′ variant P165A, 32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA
rRNA:m6A methyltransferase ErmC′variant P165A	Bacillus subtilis	S-adenosyl-L-methionine	0.016	0.0185	0.86 calc.	pH 7.5, 25 °C	PMID 12946350	Open record: rRNA:m6A methyltransferase ErmC′ variant P165A, S-adenosyl-L-methionine
SaErmC	Staphylococcus aureus	32 nt RNA (23S rRNA fragment)	—	0.0034	—		PMID 41369274	Open record: SaErmC, 32 nt RNA (23S rRNA fragment)
SaErmC	Staphylococcus aureus	32 nt RNA (23S rRNA fragment)	0.0015	—	—		PMID 41369274	Open record: SaErmC, 32 nt RNA (23S rRNA fragment)
SaErmC	Staphylococcus aureus	32 nt RNA (23S rRNA fragment)	—	—	0.45		PMID 41369274	Open record: SaErmC, 32 nt RNA (23S rRNA fragment)
SaErmC	Staphylococcus aureus	32 nt RNA (23S rRNA fragment)in the presence of JNAL-016; apparent Km	—	0.0034	—		PMID 41369274	Open record: SaErmC, 32 nt RNA (23S rRNA fragment)
SaErmC	Staphylococcus aureus	32 nt RNA (23S rRNA fragment)in the presence of JNAL-016; apparent kcat	7.3 × 10⁻⁴	—	—		PMID 41369274	Open record: SaErmC, 32 nt RNA (23S rRNA fragment)
SaErmC	Staphylococcus aureus	32 nt RNA (23S rRNA fragment)in the presence of JNAL-016; apparent kcat/Km	—	—	0.22		PMID 41369274	Open record: SaErmC, 32 nt RNA (23S rRNA fragment)