Values CatLog read from this paper

Mutational analysis defines the roles of conserved amino acid residues in the predicted catalytic pocket of the rRNA:m6A methyltransferase ErmC'

PMID 12946350doi:10.1016/s0022-2836(03)00863-5Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 16 records (32 values) from this paper: rRNA:m6A methyltransferase ErmC′ from Bacillus subtilis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 16 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.1.1.184

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
rRNA:m6A methyltransferase ErmC′	Bacillus subtilis	32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA	0.0064	7 × 10⁻⁴	9.1 calc.	pH 7.5, 25 °C	Open record: rRNA:m6A methyltransferase ErmC′, 32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA
rRNA:m6A methyltransferase ErmC′	Bacillus subtilis	S-adenosyl-L-methionine	0.066	0.0028	24 calc.	pH 7.5, 25 °C	Open record: rRNA:m6A methyltransferase ErmC′, S-adenosyl-L-methionine
rRNA:m6A methyltransferase ErmC′variant E128A	Bacillus subtilis	32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA	0.0019	3 × 10⁻⁴	6.3 calc.	pH 7.5, 25 °C	Open record: rRNA:m6A methyltransferase ErmC′ variant E128A, 32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA
rRNA:m6A methyltransferase ErmC′variant E128A	Bacillus subtilis	S-adenosyl-L-methionine	0.048	0.0045	11 calc.	pH 7.5, 25 °C	Open record: rRNA:m6A methyltransferase ErmC′ variant E128A, S-adenosyl-L-methionine
rRNA:m6A methyltransferase ErmC′variant F163A	Bacillus subtilis	32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA	0.0037	0.0014	2.6 calc.	pH 7.5, 25 °C	Open record: rRNA:m6A methyltransferase ErmC′ variant F163A, 32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA
rRNA:m6A methyltransferase ErmC′variant F163A	Bacillus subtilis	S-adenosyl-L-methionine	0.032	0.002	16 calc.	pH 7.5, 25 °C	Open record: rRNA:m6A methyltransferase ErmC′ variant F163A, S-adenosyl-L-methionine
rRNA:m6A methyltransferase ErmC′variant K166A	Bacillus subtilis	32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA	0.022	0.0041	5.4 calc.	pH 7.5, 25 °C	Open record: rRNA:m6A methyltransferase ErmC′ variant K166A, 32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA
rRNA:m6A methyltransferase ErmC′variant K166A	Bacillus subtilis	S-adenosyl-L-methionine	0.054	0.0031	17 calc.	pH 7.5, 25 °C	Open record: rRNA:m6A methyltransferase ErmC′ variant K166A, S-adenosyl-L-methionine
rRNA:m6A methyltransferase ErmC′variant K168A	Bacillus subtilis	32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA	0.0087	0.0014	6.2 calc.	pH 7.5, 25 °C	Open record: rRNA:m6A methyltransferase ErmC′ variant K168A, 32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA
rRNA:m6A methyltransferase ErmC′variant K168A	Bacillus subtilis	S-adenosyl-L-methionine	0.054	0.004	14 calc.	pH 7.5, 25 °C	Open record: rRNA:m6A methyltransferase ErmC′ variant K168A, S-adenosyl-L-methionine
rRNA:m6A methyltransferase ErmC′variant N101A	Bacillus subtilis	32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA	7 × 10⁻⁴	8 × 10⁻⁴	0.88 calc.	pH 7.5, 25 °C	Open record: rRNA:m6A methyltransferase ErmC′ variant N101A, 32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA
rRNA:m6A methyltransferase ErmC′variant N101A	Bacillus subtilis	S-adenosyl-L-methionine	0.033	0.0139	2.4 calc.	pH 7.5, 25 °C	Open record: rRNA:m6A methyltransferase ErmC′ variant N101A, S-adenosyl-L-methionine
rRNA:m6A methyltransferase ErmC′variant N11A	Bacillus subtilis	32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA	0.0023	8 × 10⁻⁴	2.9 calc.	pH 7.5, 25 °C	Open record: rRNA:m6A methyltransferase ErmC′ variant N11A, 32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA
rRNA:m6A methyltransferase ErmC′variant N11A	Bacillus subtilis	S-adenosyl-L-methionine	0.029	0.004	7.2 calc.	pH 7.5, 25 °C	Open record: rRNA:m6A methyltransferase ErmC′ variant N11A, S-adenosyl-L-methionine
rRNA:m6A methyltransferase ErmC′variant P165A	Bacillus subtilis	32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA	4 × 10⁻⁴	0.0013	0.31 calc.	pH 7.5, 25 °C	Open record: rRNA:m6A methyltransferase ErmC′ variant P165A, 32-mer RNA oligonucleotide (CGCGACGGACGGA2085AAGACCCCUAUCCGUCGCG) mimicking the adenine loop in domain V of B. subtilis 23S rRNA
rRNA:m6A methyltransferase ErmC′variant P165A	Bacillus subtilis	S-adenosyl-L-methionine	0.016	0.0185	0.86 calc.	pH 7.5, 25 °C	Open record: rRNA:m6A methyltransferase ErmC′ variant P165A, S-adenosyl-L-methionine