Values CatLog read from this paper
Structural basis for substrate specificity of Escherichia coli purine nucleoside phosphorylase
PMID 12937174doi:10.1074/jbc.m304622200Open in Browse
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CatLog read 7 records (21 values) from this paper: purine nucleoside phosphorylase (PNP) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| purine nucleoside phosphorylase (PNP) | Escherichia coli | 2-fluoro-2′-deoxyadenosine | 2.77 | 0.023 | 120 | pH 7.4, 25 °C | Open record: purine nucleoside phosphorylase (PNP), 2-fluoro-2′-deoxyadenosine |
| purine nucleoside phosphorylase (PNP) | Escherichia coli | 2-fluoroadenosine | 2.05 | 0.012 | 170 | pH 7.4, 25 °C | Open record: purine nucleoside phosphorylase (PNP), 2-fluoroadenosine |
| purine nucleoside phosphorylase (PNP) | Escherichia coli | 9-β-d-[2-deoxyribofuranosyl]-6-methylpurine | 9.7 | 0.126 | 77 | pH 7.4, 25 °C | Open record: purine nucleoside phosphorylase (PNP), 9-β-d-[2-deoxyribofuranosyl]-6-methylpurine |
| purine nucleoside phosphorylase (PNP) | Escherichia coli | 9-β-d-arabinofuranosyl-2-fluoroadenine | 0.042 | 0.958 | 0.043 | pH 7.4, 25 °C | Open record: purine nucleoside phosphorylase (PNP), 9-β-d-arabinofuranosyl-2-fluoroadenine |
| purine nucleoside phosphorylase (PNP) | Escherichia coli | 9-β-d-ribofuranosyl-6-methylthiopurine | 1.12 | 0.009 | 123 | pH 7.4, 25 °C | Open record: purine nucleoside phosphorylase (PNP), 9-β-d-ribofuranosyl-6-methylthiopurine |
| purine nucleoside phosphorylase (PNP) | Escherichia coli | adenosine | 3.32 | 0.046 | 72 | pH 7.4, 25 °C | Open record: purine nucleoside phosphorylase (PNP), adenosine |
| purine nucleoside phosphorylase (PNP) | Escherichia coli | inosine | 4 | 0.096 | 42 | pH 7.4, 25 °C | Open record: purine nucleoside phosphorylase (PNP), inosine |