EC 2.4.2.1: purine-nucleoside phosphorylase

TransferasesOpen in Browse

CatLog read 260 records for EC 2.4.2.1 from 18 papers, for 15 organisms. Names in these papers: purine nucleoside phosphorylase (human PNP, recombinant), purine nucleoside phosphorylase (hPNP), purine nucleoside phosphorylase (PfPNP), purine nucleoside phosphorylase (PNP) and 15 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 245 records for EC 2.4.2.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.4.2.1 in Browse.

Papers

Papers with records for EC 2.4.2.1
Paper	Organisms	Records
Purine nucleoside phosphorylase. 1. Structure-function studies	Homo sapiens	45
Design and directed evolution of a dideoxy purine nucleoside phosphorylase	Homo sapiens	38
Structural determinants of the 5'-methylthioinosine specificity of Plasmodium purine nucleoside phosphorylase	Plasmodium falciparum	38
Xanthosine and xanthine. Substrate properties with purine nucleoside phosphorylases, and relevance to other enzyme systems	Bos taurus, Homo sapiens	24
Purine nucleoside phosphorylase from Escherichia coli and Salmonella typhimurium. Purification and some properties	Escherichia coli, Salmonella typhimurium	15
Substrate specificity and kinetic mechanism of purine nucleoside phosphorylase from Mycobacterium tuberculosis	Mycobacterium tuberculosis	14
Purine nucleoside phosphorylase from bovine lens: purification and properties	Bos taurus (calf)	13
Purification and properties of inosine-guanosine phosphorylase from Escherichia coli K-12	Escherichia coli, Escherichia coli K-12	13
Design of an adenosine phosphorylase by active-site modification of murine purine nucleoside phosphorylase. Enzyme kinetics and molecular dynamics simulation of Asn-243 and Lys-244 substitutions of…	Mus musculus	12
Structure-activity relationship of a cold-adapted purine nucleoside phosphorylase by site-directed mutagenesis	Pseudoalteromonas sp.	8
Functional and Structural Characterization of Purine Nucleoside Phosphorylase from Kluyveromyces lactis and Its Potential Applications in Reducing Purine Content in Food	Kluyveromyces lactis	8
Structural basis for substrate specificity of Escherichia coli purine nucleoside phosphorylase	Escherichia coli	7
Effect of the phosphate substrate on drug-inhibitor binding to human purine nucleoside phosphorylase	Homo sapiens	6
Development, validation and application of a 96-well enzymatic assay based on LC-ESI-MS/MS quantification for the screening of selective inhibitors against Mycobacterium tuberculosis purine…	Aeromonas hydrophila, Homo sapiens and 1 more	6
Toxoplasma gondii purine nucleoside phosphorylase biochemical characterization, inhibitor profiles, and comparison with the Plasmodium falciparum ortholog	Toxoplasma gondii	4
Attenuated Plasmodium yoelii lacking purine nucleoside phosphorylase confer protective immunity	Plasmodium yoelii	4
Efficient Synthesis of Purine Nucleoside Analogs by a New Trimeric Purine Nucleoside Phosphorylase from Aneurinibacillus migulanus AM007	Aneurinibacillus migulanus	3
Efficient whole-cell biocatalytic synthesis of 2'-deoxy-2'-fluoroadenosine, a key building block for nucleic acid drugs	Escherichia coli	2

Records

Records for EC 2.4.2.1 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
inosine-guanosine phosphorylase (xanthosine phosphorylase)	Escherichia coli K-12	2',3'-dideoxyinosine	—	2.6	—	pH 7, 25 °C	PMID 3042752	Open record: inosine-guanosine phosphorylase (xanthosine phosphorylase), 2',3'-dideoxyinosine
inosine-guanosine phosphorylase (xanthosine phosphorylase)	Escherichia coli K-12	2'-deoxyguanosine	—	0.044	—	pH 7, 25 °C	PMID 3042752	Open record: inosine-guanosine phosphorylase (xanthosine phosphorylase), 2'-deoxyguanosine
inosine-guanosine phosphorylase (xanthosine phosphorylase)	Escherichia coli K-12	2'-deoxyinosine	—	0.062	—	pH 7, 25 °C	PMID 3042752	Open record: inosine-guanosine phosphorylase (xanthosine phosphorylase), 2'-deoxyinosine
inosine-guanosine phosphorylase (xanthosine phosphorylase)	Escherichia coli K-12	2-deoxy-alpha-D-ribose 1-phosphate	—	0.058	—	pH 7, 25 °C	PMID 3042752	Open record: inosine-guanosine phosphorylase (xanthosine phosphorylase), 2-deoxy-alpha-D-ribose 1-phosphate
inosine-guanosine phosphorylase (xanthosine phosphorylase)	Escherichia coli K-12	5'-deoxyinosine	—	0.6	—	pH 7, 25 °C	PMID 3042752	Open record: inosine-guanosine phosphorylase (xanthosine phosphorylase), 5'-deoxyinosine
inosine-guanosine phosphorylase (xanthosine phosphorylase)	Escherichia coli K-12	alpha-D-ribose 1-phosphate	—	0.059	—	pH 7, 25 °C	PMID 3042752	Open record: inosine-guanosine phosphorylase (xanthosine phosphorylase), alpha-D-ribose 1-phosphate
inosine-guanosine phosphorylase (xanthosine phosphorylase)	Escherichia coli K-12	guanine	—	0.0041	—	pH 7, 25 °C	PMID 3042752	Open record: inosine-guanosine phosphorylase (xanthosine phosphorylase), guanine
inosine-guanosine phosphorylase (xanthosine phosphorylase)	Escherichia coli K-12	guanosine	—	0.11	—	pH 7, 25 °C	PMID 3042752	Open record: inosine-guanosine phosphorylase (xanthosine phosphorylase), guanosine
inosine-guanosine phosphorylase (xanthosine phosphorylase)	Escherichia coli K-12	hypoxanthine	—	0.0033	—	pH 7, 25 °C	PMID 3042752	Open record: inosine-guanosine phosphorylase (xanthosine phosphorylase), hypoxanthine
inosine-guanosine phosphorylase (xanthosine phosphorylase)	Escherichia coli K-12	inosine	—	0.34	—	pH 7, 25 °C	PMID 3042752	Open record: inosine-guanosine phosphorylase (xanthosine phosphorylase), inosine
inosine-guanosine phosphorylase (xanthosine phosphorylase)	Escherichia coli K-12	phosphate	—	0.76	—	pH 7, 25 °C	PMID 3042752	Open record: inosine-guanosine phosphorylase (xanthosine phosphorylase), phosphate
inosine-guanosine phosphorylase (xanthosine phosphorylase)	Escherichia coli K-12	xanthosine	—	0.051	—	pH 7, 25 °C	PMID 3042752	Open record: inosine-guanosine phosphorylase (xanthosine phosphorylase), xanthosine
purine nucleoside phosphorylase	Bos taurus (calf)	2'-deoxyguanosine	45	0.024	1.9 × 10³ calc.	pH 7.4, 37 °C	PMID 1445943	Open record: purine nucleoside phosphorylase, 2'-deoxyguanosine
purine nucleoside phosphorylase	Bos taurus (calf)	2'-deoxyinosine	85	0.043	2.0 × 10³ calc.	pH 7.4, 37 °C	PMID 1445943	Open record: purine nucleoside phosphorylase, 2'-deoxyinosine
purine nucleoside phosphorylase	Bos taurus (calf)	2-deoxy-alpha-D-ribose 1-phosphatehypoxanthine + dRib 1-P; cosubstrate hypoxanthine	115	0.129	891 calc.	pH 7.4, 37 °C	PMID 1445943	Open record: purine nucleoside phosphorylase, 2-deoxy-alpha-D-ribose 1-phosphate
purine nucleoside phosphorylase	Bos taurus (calf)	2-deoxy-alpha-D-ribose 1-phosphateguanine + dRib 1-P; cosubstrate guanine	145	0.133	1.09 × 10³ calc.	pH 7.4, 37 °C	PMID 1445943	Open record: purine nucleoside phosphorylase, 2-deoxy-alpha-D-ribose 1-phosphate
purine nucleoside phosphorylase	Bos taurus (calf)	alpha-D-ribose 1-phosphatehypoxanthine + Rib 1-P; cosubstrate hypoxanthine	100	0.042	2.4 × 10³ calc.	pH 7.4, 37 °C	PMID 1445943	Open record: purine nucleoside phosphorylase, alpha-D-ribose 1-phosphate
purine nucleoside phosphorylase	Bos taurus (calf)	alpha-D-ribose 1-phosphateguanine + Rib 1-P; cosubstrate guanine	120	0.041	2.9 × 10³ calc.	pH 7.4, 37 °C	PMID 1445943	Open record: purine nucleoside phosphorylase, alpha-D-ribose 1-phosphate
purine nucleoside phosphorylase	Bos taurus (calf)	guanineguanine + Rib 1-P; cosubstrate Rib 1-P	127	0.014	9.1 × 10³ calc.	pH 7.4, 37 °C	PMID 1445943	Open record: purine nucleoside phosphorylase, guanine
purine nucleoside phosphorylase	Bos taurus (calf)	guanineguanine + dRib 1-P; cosubstrate dRib 1-P	158	0.017	9.3 × 10³ calc.	pH 7.4, 37 °C	PMID 1445943	Open record: purine nucleoside phosphorylase, guanine
purine nucleoside phosphorylase	Bos taurus (calf)	guanosine	59	0.029	2.0 × 10³ calc.	pH 7.4, 37 °C	PMID 1445943	Open record: purine nucleoside phosphorylase, guanosine
purine nucleoside phosphorylase	Bos taurus (calf)	hypoxanthinehypoxanthine + Rib 1-P; cosubstrate Rib 1-P	103	0.02	5.2 × 10³ calc.	pH 7.4, 37 °C	PMID 1445943	Open record: purine nucleoside phosphorylase, hypoxanthine
purine nucleoside phosphorylase	Bos taurus (calf)	hypoxanthinehypoxanthine + dRib 1-P; cosubstrate dRib 1-P	127	0.021	6.0 × 10³ calc.	pH 7.4, 37 °C	PMID 1445943	Open record: purine nucleoside phosphorylase, hypoxanthine
purine nucleoside phosphorylase	Bos taurus (calf)	inosine	119	0.036	3.3 × 10³ calc.	pH 7.4, 37 °C	PMID 1445943	Open record: purine nucleoside phosphorylase, inosine
purine nucleoside phosphorylase	Bos taurus (calf)	xanthosine	—	0.45	—	pH 7.4, 37 °C	PMID 1445943	Open record: purine nucleoside phosphorylase, xanthosine
purine nucleoside phosphorylase	Escherichia coli	adenineassay procedure C: nucleoside synthesis; ribose 1-P (1.0 mM)	—	0.04	—	pH 7.1, 37 °C	PMID 235429	Open record: purine nucleoside phosphorylase, adenine
purine nucleoside phosphorylase	Escherichia coli	adenineassay procedure C: nucleoside synthesis	—	0.04	—	pH 7.1, 37 °C	PMID 235429	Open record: purine nucleoside phosphorylase, adenine
purine nucleoside phosphorylase	Escherichia coli	adenineassay procedure D: ribosyl transfer	—	0.11	—	pH 7.1, 37 °C	PMID 235429	Open record: purine nucleoside phosphorylase, adenine
purine nucleoside phosphorylase	Escherichia coli	deoxyinosine	—	0.18	—	pH 7.1, 37 °C	PMID 235429	Open record: purine nucleoside phosphorylase, deoxyinosine
purine nucleoside phosphorylase	Escherichia coli	deoxyribose 1-phosphate	—	0.1	—	pH 7.1, 37 °C	PMID 235429	Open record: purine nucleoside phosphorylase, deoxyribose 1-phosphate
purine nucleoside phosphorylase	Escherichia coli	inosinepotassium phosphate buffer pH 7.1; phosphate (180 mM)	—	0.07	—	pH 7.1, 37 °C	PMID 235429	Open record: purine nucleoside phosphorylase, inosine
purine nucleoside phosphorylase	Escherichia coli	inosineassay procedure D: ribosyl transfer	—	0.55	—	pH 7.1, 37 °C	PMID 235429	Open record: purine nucleoside phosphorylase, inosine
purine nucleoside phosphorylase	Escherichia coli	ribose 1-phosphate	—	0.04	—	pH 7.1, 37 °C	PMID 235429	Open record: purine nucleoside phosphorylase, ribose 1-phosphate
purine nucleoside phosphorylasevariant N243A	Homo sapiens	phosphate	—	—	0.03	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase variant N243A, phosphate
purine nucleoside phosphorylase	Salmonella typhimurium	adenine	—	0.11	—	pH 7.1, 37 °C	PMID 235429	Open record: purine nucleoside phosphorylase, adenine
purine nucleoside phosphorylase	Salmonella typhimurium	deoxyadenosine	—	0.43	—	pH 7.1, 37 °C	PMID 235429	Open record: purine nucleoside phosphorylase, deoxyadenosine
purine nucleoside phosphorylase	Salmonella typhimurium	deoxyguanosine	—	0.14	—	pH 7.1, 37 °C	PMID 235429	Open record: purine nucleoside phosphorylase, deoxyguanosine
purine nucleoside phosphorylase	Salmonella typhimurium	deoxyinosinepotassium phosphate buffer pH 7.1	—	0.17	—	pH 7.1, 37 °C	PMID 235429	Open record: purine nucleoside phosphorylase, deoxyinosine
purine nucleoside phosphorylase	Salmonella typhimurium	deoxyinosineassay procedure B: arsenolysis of deoxyribonucleoside	—	0.21	—	pH 7.1, 37 °C	PMID 235429	Open record: purine nucleoside phosphorylase, deoxyinosine
purine nucleoside phosphorylase	Salmonella typhimurium	deoxyribose 1-phosphate	—	0.1	—	pH 7.1, 37 °C	PMID 235429	Open record: purine nucleoside phosphorylase, deoxyribose 1-phosphate
purine nucleoside phosphorylase	Salmonella typhimurium	inosine	—	0.09	—	pH 7.1, 37 °C	PMID 235429	Open record: purine nucleoside phosphorylase, inosine
purine nucleoside phosphorylase (AhPNP)	Aeromonas hydrophila	inosine	3.73 × 10⁵	1.3	2.88 × 10⁵	pH 7.5, 37 °C	PMID 27769382	Open record: purine nucleoside phosphorylase (AhPNP), inosine
purine nucleoside phosphorylase (AhPNP)	Aeromonas hydrophila	phosphate	4.43 × 10⁵	0.19	2.289 × 10⁶	pH 7.5, 37 °C	PMID 27769382	Open record: purine nucleoside phosphorylase (AhPNP), phosphate
purine nucleoside phosphorylase (AmPNP)	Aneurinibacillus migulanus	adenosine	14.3	1.45	9.92	pH 8, 50 °C	PMID 31888088	Open record: purine nucleoside phosphorylase (AmPNP), adenosine
purine nucleoside phosphorylase (AmPNP)	Aneurinibacillus migulanus	guanosine	1.84 × 10⁴	1.16	1.58 × 10⁴	pH 8, 50 °C	PMID 31888088	Open record: purine nucleoside phosphorylase (AmPNP), guanosine
purine nucleoside phosphorylase (AmPNP)	Aneurinibacillus migulanus	inosine	6.38 × 10⁴	5.3	1.21 × 10⁴	pH 8, 50 °C	PMID 31888088	Open record: purine nucleoside phosphorylase (AmPNP), inosine
purine nucleoside phosphorylase (deo-encoded)	Escherichia coli	xanthosine	—	1.6	—	25 °C	PMID 3042752	Open record: purine nucleoside phosphorylase (deo-encoded), xanthosine
purine nucleoside phosphorylase (EcPNP)	Escherichia coli	2'-deoxy-2'-fluoroadenosinenon-pretreated cells	0.1698	17.12	0.01	pH 7	PMID 42179739	Open record: purine nucleoside phosphorylase (EcPNP), 2'-deoxy-2'-fluoroadenosine
purine nucleoside phosphorylase (EcPNP)	Escherichia coli	2'-deoxy-2'-fluoroadenosinecells thermally pretreated at 50 °C for 3 h	0.1373	12.56	0.011	pH 7	PMID 42179739	Open record: purine nucleoside phosphorylase (EcPNP), 2'-deoxy-2'-fluoroadenosine
purine nucleoside phosphorylase (hPNP)	Homo sapiens	2',3'-dideoxyinosine	0.9	1.03	0.875	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP), 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)	Homo sapiens	inosine	43.9	0.048	921	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP), inosine
purine nucleoside phosphorylase (hPNP)variant G4E/Y88F/M170T/Q172L	Homo sapiens	2',3'-dideoxyinosine	6.1	0.247	24.65	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant G4E/Y88F/M170T/Q172L, 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)variant G4E/Y88F/M170T/Q172L	Homo sapiens	inosine	14.5	0.037	397	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant G4E/Y88F/M170T/Q172L, inosine
purine nucleoside phosphorylase (hPNP)variant G4E/Y88F/M170T/Q172L/T177A	Homo sapiens	2',3'-dideoxyinosine	4.5	0.235	19.24	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant G4E/Y88F/M170T/Q172L/T177A, 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)variant G4E/Y88F/M170T/Q172L/T177A	Homo sapiens	inosine	14.7	0.033	439	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant G4E/Y88F/M170T/Q172L/T177A, inosine
purine nucleoside phosphorylase (hPNP)variant Y88A	Homo sapiens	2',3'-dideoxyinosine	2.8	1.67	1.67	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88A, 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)variant Y88A	Homo sapiens	inosine	17.6	0.08	219	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88A, inosine
purine nucleoside phosphorylase (hPNP)variant Y88C	Homo sapiens	2',3'-dideoxyinosine	2.5	0.81	3.07	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88C, 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)variant Y88C	Homo sapiens	inosine	5.5	0.17	32.1	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88C, inosine
purine nucleoside phosphorylase (hPNP)variant Y88D	Homo sapiens	2',3'-dideoxyinosine	0.02	12.7	0.0018	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88D, 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)variant Y88D	Homo sapiens	inosine	0.8	2.6	0.29	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88D, inosine
purine nucleoside phosphorylase (hPNP)variant Y88E	Homo sapiens	2',3'-dideoxyinosine	0.01	14.9	9 × 10⁻⁴	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88E, 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)variant Y88E	Homo sapiens	inosine	0.7	4.86	0.14	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88E, inosine
purine nucleoside phosphorylase (hPNP)variant Y88F	Homo sapiens	2',3'-dideoxyinosine	10.5	0.45	23.14	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88F, 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)variant Y88F	Homo sapiens	inosine	28.3	0.073	389	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88F, inosine
purine nucleoside phosphorylase (hPNP)variant Y88F/M170T	Homo sapiens	2',3'-dideoxyinosine	5.8	0.23	25.16	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88F/M170T, 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)variant Y88F/M170T	Homo sapiens	inosine	9.6	0.026	371	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88F/M170T, inosine
purine nucleoside phosphorylase (hPNP)variant Y88H	Homo sapiens	2',3'-dideoxyinosine	2.5	0.675	3.67	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88H, 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)variant Y88H	Homo sapiens	inosine	8.6	0.074	116	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88H, inosine
purine nucleoside phosphorylase (hPNP)variant Y88I	Homo sapiens	2',3'-dideoxyinosine	0.5	4.67	0.115	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88I, 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)variant Y88I	Homo sapiens	inosine	1.3	1.42	0.94	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88I, inosine
purine nucleoside phosphorylase (hPNP)variant Y88L	Homo sapiens	2',3'-dideoxyinosine	6.1	0.391	15.67	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88L, 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)variant Y88L	Homo sapiens	inosine	15.9	0.094	169	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88L, inosine
purine nucleoside phosphorylase (hPNP)variant Y88M	Homo sapiens	2',3'-dideoxyinosine	5.2	0.645	8.09	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88M, 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)variant Y88M	Homo sapiens	inosine	12.8	0.345	37.1	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88M, inosine
purine nucleoside phosphorylase (hPNP)variant Y88N	Homo sapiens	2',3'-dideoxyinosine	1.5	9.53	0.153	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88N, 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)variant Y88N	Homo sapiens	inosine	9.7	0.845	11.4	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88N, inosine
purine nucleoside phosphorylase (hPNP)variant Y88Q	Homo sapiens	2',3'-dideoxyinosine	0.15	0.86	0.177	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88Q, 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)variant Y88Q	Homo sapiens	inosine	1.4	0.855	1.7	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88Q, inosine
purine nucleoside phosphorylase (hPNP)variant Y88S	Homo sapiens	2',3'-dideoxyinosine	2.7	4.06	0.652	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88S, 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)variant Y88S	Homo sapiens	inosine	8.8	0.43	20.3	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88S, inosine
purine nucleoside phosphorylase (hPNP)variant Y88T	Homo sapiens	2',3'-dideoxyinosine	0.4	8.16	0.044	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88T, 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)variant Y88T	Homo sapiens	inosine	2.3	1.2	1.9	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88T, inosine
purine nucleoside phosphorylase (hPNP)variant Y88V	Homo sapiens	2',3'-dideoxyinosine	0.6	4.41	0.144	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88V, 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)variant Y88V	Homo sapiens	inosine	2.4	1.2	1.96	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88V, inosine
purine nucleoside phosphorylase (hPNP)variant Y88W	Homo sapiens	2',3'-dideoxyinosine	0.09	0.98	0.091	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88W, 2',3'-dideoxyinosine
purine nucleoside phosphorylase (hPNP)variant Y88W	Homo sapiens	inosine	5.6	0.5	11.3	pH 7, 25 °C	PMID 20525731	Open record: purine nucleoside phosphorylase (hPNP) variant Y88W, inosine
purine nucleoside phosphorylase (hsPNP)	Homo sapiens	7-methylguanosine100 mM HEPES + 50 mM potassium phosphate buffer	9.21	0.0393	240	pH 7, 25 °C	PMID 18938130	Open record: purine nucleoside phosphorylase (hsPNP), 7-methylguanosine
purine nucleoside phosphorylase (hsPNP)	Homo sapiens	7-methylguanosine100 mM HEPES + 50 mM potassium phosphate buffer	20.4	0.0092	2256	pH 7, 25 °C	PMID 18938130	Open record: purine nucleoside phosphorylase (hsPNP), 7-methylguanosine
purine nucleoside phosphorylase (hsPNP)	Homo sapiens	7-methylguanosine100 mM HEPES + 50 mM potassium phosphate buffer	73.1	0.0125	5.86 × 10³	pH 7, 25 °C	PMID 18938130	Open record: purine nucleoside phosphorylase (hsPNP), 7-methylguanosine
purine nucleoside phosphorylase (hsPNP)	Homo sapiens	7-methylguanosine100 mM HEPES + 100 mM potassium phosphate buffer	6.23	0.013	488	pH 7, 25 °C	PMID 18938130	Open record: purine nucleoside phosphorylase (hsPNP), 7-methylguanosine
purine nucleoside phosphorylase (hsPNP)	Homo sapiens	7-methylguanosine100 mM HEPES + 100 mM potassium phosphate buffer	23	0.0139	1674	pH 7, 25 °C	PMID 18938130	Open record: purine nucleoside phosphorylase (hsPNP), 7-methylguanosine
purine nucleoside phosphorylase (hsPNP)	Homo sapiens	7-methylguanosine100 mM HEPES + 100 mM potassium phosphate buffer	24.7	0.0163	1548	pH 7, 25 °C	PMID 18938130	Open record: purine nucleoside phosphorylase (hsPNP), 7-methylguanosine
purine nucleoside phosphorylase (HsPNP)	Homo sapiens	inosine	14	0.15	90	pH 7.5, 37 °C	PMID 27769382	Open record: purine nucleoside phosphorylase (HsPNP), inosine
purine nucleoside phosphorylase (HsPNP)	Homo sapiens	phosphate	76	0.66	110	pH 7.5, 37 °C	PMID 27769382	Open record: purine nucleoside phosphorylase (HsPNP), phosphate
purine nucleoside phosphorylase (human erythrocytic PNP)	Homo sapiens	alpha-D-ribose 1-phosphate	—	0.29	—	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human erythrocytic PNP), alpha-D-ribose 1-phosphate
purine nucleoside phosphorylase (human erythrocytic PNP)	Homo sapiens	hypoxanthine	—	0.012	—	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human erythrocytic PNP), hypoxanthine
purine nucleoside phosphorylase (human erythrocytic PNP)	Homo sapiens	inosine	—	0.029	—	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human erythrocytic PNP), inosine
purine nucleoside phosphorylase (human PNP, recombinant)	Homo sapiens	hypoxanthine	110	0.03	3.7 × 10³	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant), hypoxanthine
purine nucleoside phosphorylase (human PNP, recombinant)	Homo sapiens	inosine	56	0.04	1.4 × 10³	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant), inosine
purine nucleoside phosphorylase (human PNP, recombinant)	Homo sapiens	phosphate	56	4	14	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant), phosphate
purine nucleoside phosphorylase (human PNP, recombinant)variant E201A	Homo sapiens	hypoxanthine	1.4	1	1	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant E201A, hypoxanthine
purine nucleoside phosphorylase (human PNP, recombinant)variant E201A	Homo sapiens	inosine	4.2	8.4	0.5	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant E201A, inosine
purine nucleoside phosphorylase (human PNP, recombinant)variant E201A	Homo sapiens	phosphate	1.8	6.1	0.1	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant E201A, phosphate
purine nucleoside phosphorylase (human PNP, recombinant)variant E89A	Homo sapiens	inosine	46	0.22	210	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant E89A, inosine
purine nucleoside phosphorylase (human PNP, recombinant)variant E89A	Homo sapiens	phosphate	53	91	0.6	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant E89A, phosphate
purine nucleoside phosphorylase (human PNP, recombinant)variant F159A	Homo sapiens	hypoxanthine	24	0.025	1 × 10³	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant F159A, hypoxanthine
purine nucleoside phosphorylase (human PNP, recombinant)variant F159A	Homo sapiens	inosine	32	0.31	100	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant F159A, inosine
purine nucleoside phosphorylase (human PNP, recombinant)variant F159A	Homo sapiens	phosphate	30	2.5	10	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant F159A, phosphate
purine nucleoside phosphorylase (human PNP, recombinant)variant F200A	Homo sapiens	hypoxanthine	14	0.57	24	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant F200A, hypoxanthine
purine nucleoside phosphorylase (human PNP, recombinant)variant F200A	Homo sapiens	inosine	9.4	19	0.5	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant F200A, inosine
purine nucleoside phosphorylase (human PNP, recombinant)variant F200A	Homo sapiens	phosphate	6.2	4.5	1	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant F200A, phosphate
purine nucleoside phosphorylase (human PNP, recombinant)variant H257A	Homo sapiens	hypoxanthine	10	0.02	500	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant H257A, hypoxanthine
purine nucleoside phosphorylase (human PNP, recombinant)variant H257A	Homo sapiens	inosine	16	0.21	70	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant H257A, inosine
purine nucleoside phosphorylase (human PNP, recombinant)variant H257A	Homo sapiens	phosphate	13	2.5	5	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant H257A, phosphate
purine nucleoside phosphorylase (human PNP, recombinant)variant H86A	Homo sapiens	hypoxanthine	0.97	0.019	50	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant H86A, hypoxanthine
purine nucleoside phosphorylase (human PNP, recombinant)variant H86A	Homo sapiens	inosine	14	0.28	50	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant H86A, inosine
purine nucleoside phosphorylase (human PNP, recombinant)variant H86A	Homo sapiens	phosphate	9.4	9.2	0.8	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant H86A, phosphate
purine nucleoside phosphorylase (human PNP, recombinant)variant K244A	Homo sapiens	hypoxanthine	98	0.04	2.5 × 10³	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant K244A, hypoxanthine
purine nucleoside phosphorylase (human PNP, recombinant)variant K244A	Homo sapiens	inosine	49	0.037	1.3 × 10³	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant K244A, inosine
purine nucleoside phosphorylase (human PNP, recombinant)variant K244A	Homo sapiens	phosphate	52	2.7	19	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant K244A, phosphate
purine nucleoside phosphorylase (human PNP, recombinant)variant M219A	Homo sapiens	hypoxanthine	130	0.18	720	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant M219A, hypoxanthine
purine nucleoside phosphorylase (human PNP, recombinant)variant M219A	Homo sapiens	inosine	52	8	10	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant M219A, inosine
purine nucleoside phosphorylase (human PNP, recombinant)variant M219A	Homo sapiens	phosphate	41	4.3	10	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant M219A, phosphate
purine nucleoside phosphorylase (human PNP, recombinant)variant N243A	Homo sapiens	hypoxanthine	13	2.3	6	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant N243A, hypoxanthine
purine nucleoside phosphorylase (human PNP, recombinant)variant N243A	Homo sapiens	inosine	0.06	0.022	3	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant N243A, inosine
purine nucleoside phosphorylase (human PNP, recombinant)variant N243A	Homo sapiens	phosphate	0.09	3.5	0.026 calc.	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant N243A, phosphate
purine nucleoside phosphorylase (human PNP, recombinant)variant R84A	Homo sapiens	hypoxanthine	0.5	0.02	25	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant R84A, hypoxanthine
purine nucleoside phosphorylase (human PNP, recombinant)variant R84A	Homo sapiens	inosine	2.1	0.13	16	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant R84A, inosine
purine nucleoside phosphorylase (human PNP, recombinant)variant R84A	Homo sapiens	phosphate	1.5	4.5	0.3	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant R84A, phosphate
purine nucleoside phosphorylase (human PNP, recombinant)variant S33A	Homo sapiens	hypoxanthine	2.1	0.013	150	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant S33A, hypoxanthine
purine nucleoside phosphorylase (human PNP, recombinant)variant S33A	Homo sapiens	inosine	11	0.14	80	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant S33A, inosine
purine nucleoside phosphorylase (human PNP, recombinant)variant S33A	Homo sapiens	phosphate	11	3.6	3	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant S33A, phosphate
purine nucleoside phosphorylase (human PNP, recombinant)variant T242A	Homo sapiens	hypoxanthine	58	0.024	2.4 × 10³	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant T242A, hypoxanthine
purine nucleoside phosphorylase (human PNP, recombinant)variant T242A	Homo sapiens	inosine	14	0.022	640	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant T242A, inosine
purine nucleoside phosphorylase (human PNP, recombinant)variant T242A	Homo sapiens	phosphate	12	0.22	60	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant T242A, phosphate
purine nucleoside phosphorylase (human PNP, recombinant)variant Y88F	Homo sapiens	hypoxanthine	9	0.01	900	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant Y88F, hypoxanthine
purine nucleoside phosphorylase (human PNP, recombinant)variant Y88F	Homo sapiens	inosine	54	0.058	930	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant Y88F, inosine
purine nucleoside phosphorylase (human PNP, recombinant)variant Y88F	Homo sapiens	phosphate	56	2.1	30	pH 7, 25 °C	PMID 9305962	Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant Y88F, phosphate
purine nucleoside phosphorylase (KlacPNP)	Kluyveromyces lactis	guanosine	29.7	0.5	50	pH 7, 25 °C	PMID 27768715	Open record: purine nucleoside phosphorylase (KlacPNP), guanosine
purine nucleoside phosphorylase (KlacPNP)	Kluyveromyces lactis	inosine	43.9	0.021	2 × 10³	pH 7, 25 °C	PMID 27768715	Open record: purine nucleoside phosphorylase (KlacPNP), inosine
purine nucleoside phosphorylase (KlacPNP)	Kluyveromyces lactis	xanthosine	16.8	0.555	30	pH 7, 25 °C	PMID 27768715	Open record: purine nucleoside phosphorylase (KlacPNP), xanthosine
purine nucleoside phosphorylase (KlacPNP)variant N256D	Kluyveromyces lactis	adenosine	22.8	0.0103	2.2 × 10³	pH 7, 25 °C	PMID 27768715	Open record: purine nucleoside phosphorylase (KlacPNP) variant N256D, adenosine
purine nucleoside phosphorylase (KlacPNP)variant N256D	Kluyveromyces lactis	guanosine	10.4	0.842	12	pH 7, 25 °C	PMID 27768715	Open record: purine nucleoside phosphorylase (KlacPNP) variant N256D, guanosine
purine nucleoside phosphorylase (KlacPNP)variant N256D	Kluyveromyces lactis	inosine	34.9	0.222	160	pH 7, 25 °C	PMID 27768715	Open record: purine nucleoside phosphorylase (KlacPNP) variant N256D, inosine
purine nucleoside phosphorylase (KlacPNP)variant N256D	Kluyveromyces lactis	xanthosine	23.3	0.536	43	pH 7, 25 °C	PMID 27768715	Open record: purine nucleoside phosphorylase (KlacPNP) variant N256D, xanthosine
purine nucleoside phosphorylase (KlacPNP)variant N256E	Kluyveromyces lactis	inosine	25.1	0.144	170	pH 7, 25 °C	PMID 27768715	Open record: purine nucleoside phosphorylase (KlacPNP) variant N256E, inosine
purine nucleoside phosphorylase (MtbPNP)	Mycobacterium tuberculosis	inosine	15.7	0.06	240	pH 7.5, 37 °C	PMID 27769382	Open record: purine nucleoside phosphorylase (MtbPNP), inosine
purine nucleoside phosphorylase (MtbPNP)	Mycobacterium tuberculosis	phosphate	4.5	0.15	30	pH 7.5, 37 °C	PMID 27769382	Open record: purine nucleoside phosphorylase (MtbPNP), phosphate
purine nucleoside phosphorylase (MtPNP)	Mycobacterium tuberculosis	2-amino-6-mercapto-7-methylpurine ribonucleoside	17.3	0.029	600	pH 7.5, 25 °C	PMID 19416718	Open record: purine nucleoside phosphorylase (MtPNP), 2-amino-6-mercapto-7-methylpurine ribonucleoside
purine nucleoside phosphorylase (MtPNP)	Mycobacterium tuberculosis	2′-Deoxyguanosine	5.1	0.015	340	pH 7.5, 25 °C	PMID 19416718	Open record: purine nucleoside phosphorylase (MtPNP), 2′-Deoxyguanosine
purine nucleoside phosphorylase (MtPNP)	Mycobacterium tuberculosis	2′-deoxyguanosine	4.4	0.047	94	pH 7, 25 °C	PMID 19416718	Open record: purine nucleoside phosphorylase (MtPNP), 2′-deoxyguanosine
purine nucleoside phosphorylase (MtPNP)	Mycobacterium tuberculosis	2′-Deoxyinosine	7.5	0.068	110	pH 7.5, 25 °C	PMID 19416718	Open record: purine nucleoside phosphorylase (MtPNP), 2′-Deoxyinosine
purine nucleoside phosphorylase (MtPNP)	Mycobacterium tuberculosis	7-Methylguanosine	18	0.033	550	pH 7.5, 25 °C	PMID 19416718	Open record: purine nucleoside phosphorylase (MtPNP), 7-Methylguanosine
purine nucleoside phosphorylase (MtPNP)	Mycobacterium tuberculosis	Guanosine	4	0.012	330	pH 7.5, 25 °C	PMID 19416718	Open record: purine nucleoside phosphorylase (MtPNP), Guanosine
purine nucleoside phosphorylase (MtPNP)	Mycobacterium tuberculosis	inorganic phosphatePi varied at fixed-saturating 300 μM MESG; cosubstrate MESG	17.9	0.427	42	pH 7.5, 25 °C	PMID 19416718	Open record: purine nucleoside phosphorylase (MtPNP), inorganic phosphate
purine nucleoside phosphorylase (MtPNP)	Mycobacterium tuberculosis	inorganic phosphatePi varied at fixed-saturating 100 μM guanosine	3.5	1.345	2.6	pH 7.5, 25 °C	PMID 19416718	Open record: purine nucleoside phosphorylase (MtPNP), inorganic phosphate
purine nucleoside phosphorylase (MtPNP)	Mycobacterium tuberculosis	inorganic phosphatePi varied at fixed-saturating 100 μM 2′-deoxyguanosine	4.9	2.349	2.1	pH 7.5, 25 °C	PMID 19416718	Open record: purine nucleoside phosphorylase (MtPNP), inorganic phosphate
purine nucleoside phosphorylase (MtPNP)	Mycobacterium tuberculosis	inorganic phosphatePi varied at fixed-saturating 400 μM inosine	4.9	0.583	8.4	pH 7.5, 25 °C	PMID 19416718	Open record: purine nucleoside phosphorylase (MtPNP), inorganic phosphate
purine nucleoside phosphorylase (MtPNP)	Mycobacterium tuberculosis	inorganic phosphatePi varied at fixed-saturating 400 μM 2′-deoxyinosine	6.9	1.131	6.1	pH 7.5, 25 °C	PMID 19416718	Open record: purine nucleoside phosphorylase (MtPNP), inorganic phosphate
purine nucleoside phosphorylase (MtPNP)	Mycobacterium tuberculosis	inorganic phosphatePi varied at fixed-saturating 100 μM 7-methylguanosine	13.3	0.427	31	pH 7.5, 25 °C	PMID 19416718	Open record: purine nucleoside phosphorylase (MtPNP), inorganic phosphate
purine nucleoside phosphorylase (MtPNP)	Mycobacterium tuberculosis	inorganic phosphate	—	1.372	3.2	pH 7, 25 °C	PMID 19416718	Open record: purine nucleoside phosphorylase (MtPNP), inorganic phosphate
purine nucleoside phosphorylase (MtPNP)	Mycobacterium tuberculosis	Inosine	5.4	0.04	140	pH 7.5, 25 °C	PMID 19416718	Open record: purine nucleoside phosphorylase (MtPNP), Inosine
purine nucleoside phosphorylase (PfPNP)	Plasmodium falciparum	5'-methylthioinosine	0.83	0.0088	94	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP), 5'-methylthioinosine
purine nucleoside phosphorylase (PfPNP)	Plasmodium falciparum	inosine	1.7	0.011	150	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP), inosine
purine nucleoside phosphorylase (PfPNP)variant D206A	Plasmodium falciparum	inosine	0.04	0.063	0.63	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant D206A, inosine
purine nucleoside phosphorylase (PfPNP)variant H7A	Plasmodium falciparum	5'-methylthioinosine	0.29	0.0026	11	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant H7A, 5'-methylthioinosine
purine nucleoside phosphorylase (PfPNP)variant H7A	Plasmodium falciparum	inosine	1.1	0.045	25	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant H7A, inosine
purine nucleoside phosphorylase (PfPNP)variant H7F	Plasmodium falciparum	5'-methylthioinosine	0.21	0.0096	22	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant H7F, 5'-methylthioinosine
purine nucleoside phosphorylase (PfPNP)variant H7F	Plasmodium falciparum	inosine	2.2	0.353	6.5	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant H7F, inosine
purine nucleoside phosphorylase (PfPNP)variant H7S	Plasmodium falciparum	5'-methylthioinosine	0.88	0.0032	270	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant H7S, 5'-methylthioinosine
purine nucleoside phosphorylase (PfPNP)variant H7S	Plasmodium falciparum	inosine	0.5	0.014	38	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant H7S, inosine
purine nucleoside phosphorylase (PfPNP)variant M183A	Plasmodium falciparum	inosine	0.4	0.26	1.5	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant M183A, inosine
purine nucleoside phosphorylase (PfPNP)variant R45A	Plasmodium falciparum	inosine	0.05	0.47	0.11	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant R45A, inosine
purine nucleoside phosphorylase (PfPNP)variant V66A	Plasmodium falciparum	5'-methylthioinosine	0.12	3 × 10⁻⁴	350	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V66A, 5'-methylthioinosine
purine nucleoside phosphorylase (PfPNP)variant V66A	Plasmodium falciparum	inosine	2.4	0.013	180	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V66A, inosine
purine nucleoside phosphorylase (PfPNP)variant V66F	Plasmodium falciparum	inosine	5.7	1.5	3.8	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V66F, inosine
purine nucleoside phosphorylase (PfPNP)variant V66I	Plasmodium falciparum	5'-methylthioinosine	1.2	0.012	94	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V66I, 5'-methylthioinosine
purine nucleoside phosphorylase (PfPNP)variant V66I	Plasmodium falciparum	inosine	2	0.016	130	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V66I, inosine
purine nucleoside phosphorylase (PfPNP)variant V66I/V73I	Plasmodium falciparum	5'-methylthioinosine	0.91	0.015	62	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V66I/V73I, 5'-methylthioinosine
purine nucleoside phosphorylase (PfPNP)variant V66I/V73I	Plasmodium falciparum	inosine	4.6	0.028	170	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V66I/V73I, inosine
purine nucleoside phosphorylase (PfPNP)variant V66I/V73I/Y160F	Plasmodium falciparum	5'-methylthioinosine	0.01	0.018	0.56	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V66I/V73I/Y160F, 5'-methylthioinosine
purine nucleoside phosphorylase (PfPNP)variant V66I/V73I/Y160F	Plasmodium falciparum	inosine	0.36	0.004	90	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V66I/V73I/Y160F, inosine
purine nucleoside phosphorylase (PfPNP)variant V66I/Y160F	Plasmodium falciparum	5'-methylthioinosine	0.05	0.011	4.7	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V66I/Y160F, 5'-methylthioinosine
purine nucleoside phosphorylase (PfPNP)variant V66I/Y160F	Plasmodium falciparum	inosine	1.2	0.0062	190	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V66I/Y160F, inosine
purine nucleoside phosphorylase (PfPNP)variant V66S	Plasmodium falciparum	5'-methylthioinosine	0.21	7 × 10⁻⁴	280	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V66S, 5'-methylthioinosine
purine nucleoside phosphorylase (PfPNP)variant V66S	Plasmodium falciparum	inosine	1.4	0.078	18	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V66S, inosine
purine nucleoside phosphorylase (PfPNP)variant V73A	Plasmodium falciparum	5'-methylthioinosine	1.9	0.02	97	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V73A, 5'-methylthioinosine
purine nucleoside phosphorylase (PfPNP)variant V73A	Plasmodium falciparum	inosine	1.1	0.007	160	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V73A, inosine
purine nucleoside phosphorylase (PfPNP)variant V73I	Plasmodium falciparum	5'-methylthioinosine	0.81	0.011	71	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V73I, 5'-methylthioinosine
purine nucleoside phosphorylase (PfPNP)variant V73I	Plasmodium falciparum	inosine	1.9	0.013	140	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V73I, inosine
purine nucleoside phosphorylase (PfPNP)variant V73I/Y160F	Plasmodium falciparum	5'-methylthioinosine	0.28	0.06	4.6	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V73I/Y160F, 5'-methylthioinosine
purine nucleoside phosphorylase (PfPNP)variant V73I/Y160F	Plasmodium falciparum	inosine	0.59	0.0036	160	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V73I/Y160F, inosine
purine nucleoside phosphorylase (PfPNP)variant V73S	Plasmodium falciparum	5'-methylthioinosine	0.02	2.1	0.011	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V73S, 5'-methylthioinosine
purine nucleoside phosphorylase (PfPNP)variant V73S	Plasmodium falciparum	inosine	0.2	0.005	46	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant V73S, inosine
purine nucleoside phosphorylase (PfPNP)variant Y160A	Plasmodium falciparum	5'-methylthioinosine	0.09	0.2	0.45	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant Y160A, 5'-methylthioinosine
purine nucleoside phosphorylase (PfPNP)variant Y160A	Plasmodium falciparum	inosine	0.1	5.4	0.22	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant Y160A, inosine
purine nucleoside phosphorylase (PfPNP)variant Y160F	Plasmodium falciparum	5'-methylthioinosine	0.3	0.044	6.8	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant Y160F, 5'-methylthioinosine
purine nucleoside phosphorylase (PfPNP)variant Y160F	Plasmodium falciparum	inosine	0.21	0.0025	83	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant Y160F, inosine
purine nucleoside phosphorylase (PfPNP)variant Y47A	Plasmodium falciparum	5'-methylthioinosine	0.02	0.96	0.021	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant Y47A, 5'-methylthioinosine
purine nucleoside phosphorylase (PfPNP)variant Y47A	Plasmodium falciparum	inosine	0.04	0.2	0.19	pH 7.4	PMID 24416224	Open record: purine nucleoside phosphorylase (PfPNP) variant Y47A, inosine
purine nucleoside phosphorylase (PNP)	Bos taurus	guanine	—	0.011	—	pH 6, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), guanine
purine nucleoside phosphorylase (PNP)	Bos taurus	guanine	—	0.006	—	pH 7, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), guanine
purine nucleoside phosphorylase (PNP)	Bos taurus	guanine	—	0.009	—	pH 7.5, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), guanine
purine nucleoside phosphorylase (PNP)	Bos taurus	guanine	23	—	3.8 × 10³	pH 7, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), guanine
purine nucleoside phosphorylase (PNP)	Bos taurus	guanosine	—	0.046	—	pH 5.7, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), guanosine
purine nucleoside phosphorylase (PNP)	Bos taurus	guanosine	—	0.0159	—	pH 6.5, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), guanosine
purine nucleoside phosphorylase (PNP)	Bos taurus	guanosine	—	0.011	—	pH 7, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), guanosine
purine nucleoside phosphorylase (PNP)	Bos taurus	guanosine	31	—	2.8 × 10³	pH 7, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), guanosine
purine nucleoside phosphorylase (PNP)	Bos taurus	xanthine	—	0.28	—	pH 6, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), xanthine
purine nucleoside phosphorylase (PNP)	Bos taurus	xanthine	—	0.306	—	pH 7.5, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), xanthine
purine nucleoside phosphorylase (PNP)	Bos taurus	xanthosine	—	0.4	—	pH 5.7, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), xanthosine
purine nucleoside phosphorylase (PNP)	Bos taurus	xanthosine	—	1.1	—	pH 6.5, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), xanthosine
purine nucleoside phosphorylase (PNP)	Escherichia coli	2-fluoro-2′-deoxyadenosine	2.77	0.023	120	pH 7.4, 25 °C	PMID 12937174	Open record: purine nucleoside phosphorylase (PNP), 2-fluoro-2′-deoxyadenosine
purine nucleoside phosphorylase (PNP)	Escherichia coli	2-fluoroadenosine	2.05	0.012	170	pH 7.4, 25 °C	PMID 12937174	Open record: purine nucleoside phosphorylase (PNP), 2-fluoroadenosine
purine nucleoside phosphorylase (PNP)	Escherichia coli	9-β-d-[2-deoxyribofuranosyl]-6-methylpurine	9.7	0.126	77	pH 7.4, 25 °C	PMID 12937174	Open record: purine nucleoside phosphorylase (PNP), 9-β-d-[2-deoxyribofuranosyl]-6-methylpurine
purine nucleoside phosphorylase (PNP)	Escherichia coli	9-β-d-arabinofuranosyl-2-fluoroadenine	0.042	0.958	0.043	pH 7.4, 25 °C	PMID 12937174	Open record: purine nucleoside phosphorylase (PNP), 9-β-d-arabinofuranosyl-2-fluoroadenine
purine nucleoside phosphorylase (PNP)	Escherichia coli	9-β-d-ribofuranosyl-6-methylthiopurine	1.12	0.009	123	pH 7.4, 25 °C	PMID 12937174	Open record: purine nucleoside phosphorylase (PNP), 9-β-d-ribofuranosyl-6-methylthiopurine
purine nucleoside phosphorylase (PNP)	Escherichia coli	adenosine	3.32	0.046	72	pH 7.4, 25 °C	PMID 12937174	Open record: purine nucleoside phosphorylase (PNP), adenosine
purine nucleoside phosphorylase (PNP)	Escherichia coli	inosine	4	0.096	42	pH 7.4, 25 °C	PMID 12937174	Open record: purine nucleoside phosphorylase (PNP), inosine
purine nucleoside phosphorylase (PNP)	Homo sapiens	guanine	—	0.029	—	pH 6, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), guanine
purine nucleoside phosphorylase (PNP)	Homo sapiens	guanine	—	0.0138	—	pH 7, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), guanine
purine nucleoside phosphorylase (PNP)	Homo sapiens	guanine	—	0.022	—	pH 7.5, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), guanine
purine nucleoside phosphorylase (PNP)	Homo sapiens	guanine	43	—	3.1 × 10³	pH 7, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), guanine
purine nucleoside phosphorylase (PNP)	Homo sapiens	guanosine	—	0.091	—	pH 5.7, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), guanosine
purine nucleoside phosphorylase (PNP)	Homo sapiens	guanosine	—	0.014	—	pH 6.5, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), guanosine
purine nucleoside phosphorylase (PNP)	Homo sapiens	guanosine	—	0.012	—	pH 7, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), guanosine
purine nucleoside phosphorylase (PNP)	Homo sapiens	guanosine	33	—	2.8 × 10³	pH 7, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), guanosine
purine nucleoside phosphorylase (PNP)	Homo sapiens	xanthine	—	0.38	—	pH 6, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), xanthine
purine nucleoside phosphorylase (PNP)	Homo sapiens	xanthine	—	0.425	—	pH 7.5, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), xanthine
purine nucleoside phosphorylase (PNP)	Homo sapiens	xanthosine	—	0.58	—	pH 5.7, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), xanthosine
purine nucleoside phosphorylase (PNP)	Homo sapiens	xanthosine	—	1.6	—	pH 6.5, 25 °C	PMID 12180982	Open record: purine nucleoside phosphorylase (PNP), xanthosine
purine nucleoside phosphorylase (PNP)	Plasmodium yoelii	2′-deoxyinosine	1.2	0.079	15	pH 7.4	PMID 18758447	Open record: purine nucleoside phosphorylase (PNP), 2′-deoxyinosine
purine nucleoside phosphorylase (PNP)	Plasmodium yoelii	5'-methylthioinosine	3.4	0.005	680	pH 7.4	PMID 18758447	Open record: purine nucleoside phosphorylase (PNP), 5'-methylthioinosine
purine nucleoside phosphorylase (PNP)	Plasmodium yoelii	Guanosine	3.8	0.0089	420	pH 7.4	PMID 18758447	Open record: purine nucleoside phosphorylase (PNP), Guanosine
purine nucleoside phosphorylase (PNP)	Plasmodium yoelii	Inosine	3.5	0.0086	400	pH 7.4	PMID 18758447	Open record: purine nucleoside phosphorylase (PNP), Inosine
purine nucleoside phosphorylase (PNP), Npb allele	Mus musculus	inosine	47.3	0.058	815	pH 7, 37 °C	PMID 10567244	Open record: purine nucleoside phosphorylase (PNP), Npb allele, inosine
purine nucleoside phosphorylase (PNP), Npb allele	Mus musculus	inosine	38.9	0.032	1217	pH 6, 37 °C	PMID 10567244	Open record: purine nucleoside phosphorylase (PNP), Npb allele, inosine
purine nucleoside phosphorylase (PNP), Npb allelevariant K244Q	Mus musculus	inosine	31	0.0459	676	pH 7, 37 °C	PMID 10567244	Open record: purine nucleoside phosphorylase (PNP), Npb allele variant K244Q, inosine
purine nucleoside phosphorylase (PNP), Npb allelevariant N243D	Mus musculus	adenosine	0.282	0.0454	6.21	pH 7, 37 °C	PMID 10567244	Open record: purine nucleoside phosphorylase (PNP), Npb allele variant N243D, adenosine
purine nucleoside phosphorylase (PNP), Npb allelevariant N243D	Mus musculus	adenosine	0.71	0.045	15.8	pH 6, 37 °C	PMID 10567244	Open record: purine nucleoside phosphorylase (PNP), Npb allele variant N243D, adenosine
purine nucleoside phosphorylase (PNP), Npb allelevariant N243D	Mus musculus	inosine	0.386	0.484	0.798	pH 7, 37 °C	PMID 10567244	Open record: purine nucleoside phosphorylase (PNP), Npb allele variant N243D, inosine
purine nucleoside phosphorylase (PNP), Npb allelevariant N243D	Mus musculus	inosine	1.76	0.691	2.55	pH 6, 37 °C	PMID 10567244	Open record: purine nucleoside phosphorylase (PNP), Npb allele variant N243D, inosine
purine nucleoside phosphorylase (PNP), Npb allelevariant N243D/K244Q	Mus musculus	adenosine	0.579	0.0417	13.8	pH 7, 37 °C	PMID 10567244	Open record: purine nucleoside phosphorylase (PNP), Npb allele variant N243D/K244Q, adenosine
purine nucleoside phosphorylase (PNP), Npb allelevariant N243D/K244Q	Mus musculus	adenosine	1.41	0.028	50.4	pH 6, 37 °C	PMID 10567244	Open record: purine nucleoside phosphorylase (PNP), Npb allele variant N243D/K244Q, adenosine
purine nucleoside phosphorylase (PNP), Npb allelevariant N243D/K244Q	Mus musculus	inosine	9.1	0.823	11.1	pH 7, 37 °C	PMID 10567244	Open record: purine nucleoside phosphorylase (PNP), Npb allele variant N243D/K244Q, inosine
purine nucleoside phosphorylase (PNP), Npb allelevariant N243D/K244Q	Mus musculus	inosine	36.4	0.729	49.9	pH 6, 37 °C	PMID 10567244	Open record: purine nucleoside phosphorylase (PNP), Npb allele variant N243D/K244Q, inosine
purine nucleoside phosphorylase (PNP), Npb allelevariant N243T	Mus musculus	inosine	0.024	0.054	0.445	pH 7, 37 °C	PMID 10567244	Open record: purine nucleoside phosphorylase (PNP), Npb allele variant N243T, inosine
purine nucleoside phosphorylase (PsPNP)variant T156A	Pseudoalteromonas sp.	inosine	56.4	1.35	41.8	pH 7.6, 37 °C	PMID 22579392	Open record: purine nucleoside phosphorylase (PsPNP) variant T156A, inosine
purine nucleoside phosphorylase (PsPNP)variant T156S	Pseudoalteromonas sp.	inosine	98.4	0.03	3.54 × 10³	pH 7.6, 37 °C	PMID 22579392	Open record: purine nucleoside phosphorylase (PsPNP) variant T156S, inosine
purine nucleoside phosphorylase (PsPNP)variant T90A	Pseudoalteromonas sp.	inosine	1.2	143.1	0.00867	pH 7.6, 37 °C	PMID 22579392	Open record: purine nucleoside phosphorylase (PsPNP) variant T90A, inosine
purine nucleoside phosphorylase (PsPNP)variant T90A/T156A	Pseudoalteromonas sp.	inosine	0.9	104.6	0.00822	pH 7.6, 37 °C	PMID 22579392	Open record: purine nucleoside phosphorylase (PsPNP) variant T90A/T156A, inosine
purine nucleoside phosphorylase (PsPNP)variant T90R	Pseudoalteromonas sp.	inosine	164.4	0.02	1.15 × 10⁴	pH 7.6, 37 °C	PMID 22579392	Open record: purine nucleoside phosphorylase (PsPNP) variant T90R, inosine
purine nucleoside phosphorylase (PsPNP)variant T90R/T156S	Pseudoalteromonas sp.	inosine	324.3	0.01	4.35 × 10⁴	pH 7.6, 37 °C	PMID 22579392	Open record: purine nucleoside phosphorylase (PsPNP) variant T90R/T156S, inosine
purine nucleoside phosphorylase (PsPNP)variant T90S	Pseudoalteromonas sp.	inosine	62.3	0.92	67.8	pH 7.6, 37 °C	PMID 22579392	Open record: purine nucleoside phosphorylase (PsPNP) variant T90S, inosine
purine nucleoside phosphorylase (PsPNP)variant V206I	Pseudoalteromonas sp.	inosine	82.4	—	189	pH 7.6, 37 °C	PMID 22579392	Open record: purine nucleoside phosphorylase (PsPNP) variant V206I, inosine
purine nucleoside phosphorylase (TgPNP)	Toxoplasma gondii	2'-deoxyinosine	0.48	0.259	1.85	pH 7.4	PMID 16829527	Open record: purine nucleoside phosphorylase (TgPNP), 2'-deoxyinosine
purine nucleoside phosphorylase (TgPNP)	Toxoplasma gondii	5'-methylthioinosine	0.027	0.0319	0.846	pH 7.4	PMID 16829527	Open record: purine nucleoside phosphorylase (TgPNP), 5'-methylthioinosine
purine nucleoside phosphorylase (TgPNP)	Toxoplasma gondii	guanosine	3.6	0.0094	383	pH 7.4	PMID 16829527	Open record: purine nucleoside phosphorylase (TgPNP), guanosine
purine nucleoside phosphorylase (TgPNP)	Toxoplasma gondii	inosine	2.6	0.0131	198	pH 7.4	PMID 16829527	Open record: purine nucleoside phosphorylase (TgPNP), inosine