Values CatLog read from this paper
Substrate specificity and kinetic mechanism of purine nucleoside phosphorylase from Mycobacterium tuberculosis
PMID 19416718doi:10.1016/j.abb.2009.04.011Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (41 values) from this paper: purine nucleoside phosphorylase (MtPNP) from Mycobacterium tuberculosis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| purine nucleoside phosphorylase (MtPNP) | Mycobacterium tuberculosis | 2-amino-6-mercapto-7-methylpurine ribonucleoside | 17.3 | 0.029 | 600 | pH 7.5, 25 °C | Open record: purine nucleoside phosphorylase (MtPNP), 2-amino-6-mercapto-7-methylpurine ribonucleoside |
| purine nucleoside phosphorylase (MtPNP) | Mycobacterium tuberculosis | 2′-Deoxyguanosine | 5.1 | 0.015 | 340 | pH 7.5, 25 °C | Open record: purine nucleoside phosphorylase (MtPNP), 2′-Deoxyguanosine |
| purine nucleoside phosphorylase (MtPNP) | Mycobacterium tuberculosis | 2′-deoxyguanosine | 4.4 | 0.047 | 94 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (MtPNP), 2′-deoxyguanosine |
| purine nucleoside phosphorylase (MtPNP) | Mycobacterium tuberculosis | 2′-Deoxyinosine | 7.5 | 0.068 | 110 | pH 7.5, 25 °C | Open record: purine nucleoside phosphorylase (MtPNP), 2′-Deoxyinosine |
| purine nucleoside phosphorylase (MtPNP) | Mycobacterium tuberculosis | 7-Methylguanosine | 18 | 0.033 | 550 | pH 7.5, 25 °C | Open record: purine nucleoside phosphorylase (MtPNP), 7-Methylguanosine |
| purine nucleoside phosphorylase (MtPNP) | Mycobacterium tuberculosis | Guanosine | 4 | 0.012 | 330 | pH 7.5, 25 °C | Open record: purine nucleoside phosphorylase (MtPNP), Guanosine |
| purine nucleoside phosphorylase (MtPNP) | Mycobacterium tuberculosis | inorganic phosphatePi varied at fixed-saturating 300 μM MESG; cosubstrate MESG | 17.9 | 0.427 | 42 | pH 7.5, 25 °C | Open record: purine nucleoside phosphorylase (MtPNP), inorganic phosphate |
| purine nucleoside phosphorylase (MtPNP) | Mycobacterium tuberculosis | inorganic phosphatePi varied at fixed-saturating 100 μM guanosine | 3.5 | 1.345 | 2.6 | pH 7.5, 25 °C | Open record: purine nucleoside phosphorylase (MtPNP), inorganic phosphate |
| purine nucleoside phosphorylase (MtPNP) | Mycobacterium tuberculosis | inorganic phosphatePi varied at fixed-saturating 100 μM 2′-deoxyguanosine | 4.9 | 2.349 | 2.1 | pH 7.5, 25 °C | Open record: purine nucleoside phosphorylase (MtPNP), inorganic phosphate |
| purine nucleoside phosphorylase (MtPNP) | Mycobacterium tuberculosis | inorganic phosphatePi varied at fixed-saturating 400 μM inosine | 4.9 | 0.583 | 8.4 | pH 7.5, 25 °C | Open record: purine nucleoside phosphorylase (MtPNP), inorganic phosphate |
| purine nucleoside phosphorylase (MtPNP) | Mycobacterium tuberculosis | inorganic phosphatePi varied at fixed-saturating 400 μM 2′-deoxyinosine | 6.9 | 1.131 | 6.1 | pH 7.5, 25 °C | Open record: purine nucleoside phosphorylase (MtPNP), inorganic phosphate |
| purine nucleoside phosphorylase (MtPNP) | Mycobacterium tuberculosis | inorganic phosphatePi varied at fixed-saturating 100 μM 7-methylguanosine | 13.3 | 0.427 | 31 | pH 7.5, 25 °C | Open record: purine nucleoside phosphorylase (MtPNP), inorganic phosphate |
| purine nucleoside phosphorylase (MtPNP) | Mycobacterium tuberculosis | inorganic phosphate | — | 1.372 | 3.2 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (MtPNP), inorganic phosphate |
| purine nucleoside phosphorylase (MtPNP) | Mycobacterium tuberculosis | Inosine | 5.4 | 0.04 | 140 | pH 7.5, 25 °C | Open record: purine nucleoside phosphorylase (MtPNP), Inosine |