Values CatLog read from this paper
Structure-activity relationship of a cold-adapted purine nucleoside phosphorylase by site-directed mutagenesis
PMID 22579392doi:10.1016/j.enzmictec.2012.04.002Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (23 values) from this paper: purine nucleoside phosphorylase (PsPNP) from Pseudoalteromonas sp..
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| purine nucleoside phosphorylase (PsPNP)variant T156A | Pseudoalteromonas sp. | inosine | 56.4 | 1.35 | 41.8 | pH 7.6, 37 °C | Open record: purine nucleoside phosphorylase (PsPNP) variant T156A, inosine |
| purine nucleoside phosphorylase (PsPNP)variant T156S | Pseudoalteromonas sp. | inosine | 98.4 | 0.03 | 3.54 × 10³ | pH 7.6, 37 °C | Open record: purine nucleoside phosphorylase (PsPNP) variant T156S, inosine |
| purine nucleoside phosphorylase (PsPNP)variant T90A | Pseudoalteromonas sp. | inosine | 1.2 | 143.1 | 0.00867 | pH 7.6, 37 °C | Open record: purine nucleoside phosphorylase (PsPNP) variant T90A, inosine |
| purine nucleoside phosphorylase (PsPNP)variant T90A/T156A | Pseudoalteromonas sp. | inosine | 0.9 | 104.6 | 0.00822 | pH 7.6, 37 °C | Open record: purine nucleoside phosphorylase (PsPNP) variant T90A/T156A, inosine |
| purine nucleoside phosphorylase (PsPNP)variant T90R | Pseudoalteromonas sp. | inosine | 164.4 | 0.02 | 1.15 × 10⁴ | pH 7.6, 37 °C | Open record: purine nucleoside phosphorylase (PsPNP) variant T90R, inosine |
| purine nucleoside phosphorylase (PsPNP)variant T90R/T156S | Pseudoalteromonas sp. | inosine | 324.3 | 0.01 | 4.35 × 10⁴ | pH 7.6, 37 °C | Open record: purine nucleoside phosphorylase (PsPNP) variant T90R/T156S, inosine |
| purine nucleoside phosphorylase (PsPNP)variant T90S | Pseudoalteromonas sp. | inosine | 62.3 | 0.92 | 67.8 | pH 7.6, 37 °C | Open record: purine nucleoside phosphorylase (PsPNP) variant T90S, inosine |
| purine nucleoside phosphorylase (PsPNP)variant V206I | Pseudoalteromonas sp. | inosine | 82.4 | — | 189 | pH 7.6, 37 °C | Open record: purine nucleoside phosphorylase (PsPNP) variant V206I, inosine |