Values CatLog read from this paper
Design and directed evolution of a dideoxy purine nucleoside phosphorylase
PMID 20525731doi:10.1093/protein/gzq033Open in Browse
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CatLog read 38 records (114 values) from this paper: purine nucleoside phosphorylase (hPNP) from Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| purine nucleoside phosphorylase (hPNP) | Homo sapiens | 2',3'-dideoxyinosine | 0.9 | 1.03 | 0.875 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP), 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP) | Homo sapiens | inosine | 43.9 | 0.048 | 921 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP), inosine |
| purine nucleoside phosphorylase (hPNP)variant G4E/Y88F/M170T/Q172L | Homo sapiens | 2',3'-dideoxyinosine | 6.1 | 0.247 | 24.65 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant G4E/Y88F/M170T/Q172L, 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP)variant G4E/Y88F/M170T/Q172L | Homo sapiens | inosine | 14.5 | 0.037 | 397 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant G4E/Y88F/M170T/Q172L, inosine |
| purine nucleoside phosphorylase (hPNP)variant G4E/Y88F/M170T/Q172L/T177A | Homo sapiens | 2',3'-dideoxyinosine | 4.5 | 0.235 | 19.24 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant G4E/Y88F/M170T/Q172L/T177A, 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP)variant G4E/Y88F/M170T/Q172L/T177A | Homo sapiens | inosine | 14.7 | 0.033 | 439 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant G4E/Y88F/M170T/Q172L/T177A, inosine |
| purine nucleoside phosphorylase (hPNP)variant Y88A | Homo sapiens | 2',3'-dideoxyinosine | 2.8 | 1.67 | 1.67 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88A, 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP)variant Y88A | Homo sapiens | inosine | 17.6 | 0.08 | 219 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88A, inosine |
| purine nucleoside phosphorylase (hPNP)variant Y88C | Homo sapiens | 2',3'-dideoxyinosine | 2.5 | 0.81 | 3.07 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88C, 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP)variant Y88C | Homo sapiens | inosine | 5.5 | 0.17 | 32.1 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88C, inosine |
| purine nucleoside phosphorylase (hPNP)variant Y88D | Homo sapiens | 2',3'-dideoxyinosine | 0.02 | 12.7 | 0.0018 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88D, 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP)variant Y88D | Homo sapiens | inosine | 0.8 | 2.6 | 0.29 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88D, inosine |
| purine nucleoside phosphorylase (hPNP)variant Y88E | Homo sapiens | 2',3'-dideoxyinosine | 0.01 | 14.9 | 9 × 10⁻⁴ | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88E, 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP)variant Y88E | Homo sapiens | inosine | 0.7 | 4.86 | 0.14 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88E, inosine |
| purine nucleoside phosphorylase (hPNP)variant Y88F | Homo sapiens | 2',3'-dideoxyinosine | 10.5 | 0.45 | 23.14 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88F, 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP)variant Y88F | Homo sapiens | inosine | 28.3 | 0.073 | 389 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88F, inosine |
| purine nucleoside phosphorylase (hPNP)variant Y88F/M170T | Homo sapiens | 2',3'-dideoxyinosine | 5.8 | 0.23 | 25.16 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88F/M170T, 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP)variant Y88F/M170T | Homo sapiens | inosine | 9.6 | 0.026 | 371 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88F/M170T, inosine |
| purine nucleoside phosphorylase (hPNP)variant Y88H | Homo sapiens | 2',3'-dideoxyinosine | 2.5 | 0.675 | 3.67 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88H, 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP)variant Y88H | Homo sapiens | inosine | 8.6 | 0.074 | 116 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88H, inosine |
| purine nucleoside phosphorylase (hPNP)variant Y88I | Homo sapiens | 2',3'-dideoxyinosine | 0.5 | 4.67 | 0.115 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88I, 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP)variant Y88I | Homo sapiens | inosine | 1.3 | 1.42 | 0.94 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88I, inosine |
| purine nucleoside phosphorylase (hPNP)variant Y88L | Homo sapiens | 2',3'-dideoxyinosine | 6.1 | 0.391 | 15.67 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88L, 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP)variant Y88L | Homo sapiens | inosine | 15.9 | 0.094 | 169 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88L, inosine |
| purine nucleoside phosphorylase (hPNP)variant Y88M | Homo sapiens | 2',3'-dideoxyinosine | 5.2 | 0.645 | 8.09 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88M, 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP)variant Y88M | Homo sapiens | inosine | 12.8 | 0.345 | 37.1 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88M, inosine |
| purine nucleoside phosphorylase (hPNP)variant Y88N | Homo sapiens | 2',3'-dideoxyinosine | 1.5 | 9.53 | 0.153 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88N, 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP)variant Y88N | Homo sapiens | inosine | 9.7 | 0.845 | 11.4 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88N, inosine |
| purine nucleoside phosphorylase (hPNP)variant Y88Q | Homo sapiens | 2',3'-dideoxyinosine | 0.15 | 0.86 | 0.177 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88Q, 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP)variant Y88Q | Homo sapiens | inosine | 1.4 | 0.855 | 1.7 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88Q, inosine |
| purine nucleoside phosphorylase (hPNP)variant Y88S | Homo sapiens | 2',3'-dideoxyinosine | 2.7 | 4.06 | 0.652 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88S, 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP)variant Y88S | Homo sapiens | inosine | 8.8 | 0.43 | 20.3 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88S, inosine |
| purine nucleoside phosphorylase (hPNP)variant Y88T | Homo sapiens | 2',3'-dideoxyinosine | 0.4 | 8.16 | 0.044 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88T, 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP)variant Y88T | Homo sapiens | inosine | 2.3 | 1.2 | 1.9 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88T, inosine |
| purine nucleoside phosphorylase (hPNP)variant Y88V | Homo sapiens | 2',3'-dideoxyinosine | 0.6 | 4.41 | 0.144 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88V, 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP)variant Y88V | Homo sapiens | inosine | 2.4 | 1.2 | 1.96 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88V, inosine |
| purine nucleoside phosphorylase (hPNP)variant Y88W | Homo sapiens | 2',3'-dideoxyinosine | 0.09 | 0.98 | 0.091 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88W, 2',3'-dideoxyinosine |
| purine nucleoside phosphorylase (hPNP)variant Y88W | Homo sapiens | inosine | 5.6 | 0.5 | 11.3 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (hPNP) variant Y88W, inosine |