Values CatLog read from this paper
Structural determinants of the 5'-methylthioinosine specificity of Plasmodium purine nucleoside phosphorylase
PMID 24416224doi:10.1371/journal.pone.0084384Open in Browse
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CatLog read 38 records (114 values) from this paper: purine nucleoside phosphorylase (PfPNP) from Plasmodium falciparum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| purine nucleoside phosphorylase (PfPNP) | Plasmodium falciparum | 5'-methylthioinosine | 0.83 | 0.0088 | 94 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP), 5'-methylthioinosine |
| purine nucleoside phosphorylase (PfPNP) | Plasmodium falciparum | inosine | 1.7 | 0.011 | 150 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP), inosine |
| purine nucleoside phosphorylase (PfPNP)variant D206A | Plasmodium falciparum | inosine | 0.04 | 0.063 | 0.63 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant D206A, inosine |
| purine nucleoside phosphorylase (PfPNP)variant H7A | Plasmodium falciparum | 5'-methylthioinosine | 0.29 | 0.0026 | 11 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant H7A, 5'-methylthioinosine |
| purine nucleoside phosphorylase (PfPNP)variant H7A | Plasmodium falciparum | inosine | 1.1 | 0.045 | 25 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant H7A, inosine |
| purine nucleoside phosphorylase (PfPNP)variant H7F | Plasmodium falciparum | 5'-methylthioinosine | 0.21 | 0.0096 | 22 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant H7F, 5'-methylthioinosine |
| purine nucleoside phosphorylase (PfPNP)variant H7F | Plasmodium falciparum | inosine | 2.2 | 0.353 | 6.5 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant H7F, inosine |
| purine nucleoside phosphorylase (PfPNP)variant H7S | Plasmodium falciparum | 5'-methylthioinosine | 0.88 | 0.0032 | 270 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant H7S, 5'-methylthioinosine |
| purine nucleoside phosphorylase (PfPNP)variant H7S | Plasmodium falciparum | inosine | 0.5 | 0.014 | 38 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant H7S, inosine |
| purine nucleoside phosphorylase (PfPNP)variant M183A | Plasmodium falciparum | inosine | 0.4 | 0.26 | 1.5 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant M183A, inosine |
| purine nucleoside phosphorylase (PfPNP)variant R45A | Plasmodium falciparum | inosine | 0.05 | 0.47 | 0.11 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant R45A, inosine |
| purine nucleoside phosphorylase (PfPNP)variant V66A | Plasmodium falciparum | 5'-methylthioinosine | 0.12 | 3 × 10⁻⁴ | 350 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V66A, 5'-methylthioinosine |
| purine nucleoside phosphorylase (PfPNP)variant V66A | Plasmodium falciparum | inosine | 2.4 | 0.013 | 180 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V66A, inosine |
| purine nucleoside phosphorylase (PfPNP)variant V66F | Plasmodium falciparum | inosine | 5.7 | 1.5 | 3.8 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V66F, inosine |
| purine nucleoside phosphorylase (PfPNP)variant V66I | Plasmodium falciparum | 5'-methylthioinosine | 1.2 | 0.012 | 94 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V66I, 5'-methylthioinosine |
| purine nucleoside phosphorylase (PfPNP)variant V66I | Plasmodium falciparum | inosine | 2 | 0.016 | 130 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V66I, inosine |
| purine nucleoside phosphorylase (PfPNP)variant V66I/V73I | Plasmodium falciparum | 5'-methylthioinosine | 0.91 | 0.015 | 62 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V66I/V73I, 5'-methylthioinosine |
| purine nucleoside phosphorylase (PfPNP)variant V66I/V73I | Plasmodium falciparum | inosine | 4.6 | 0.028 | 170 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V66I/V73I, inosine |
| purine nucleoside phosphorylase (PfPNP)variant V66I/V73I/Y160F | Plasmodium falciparum | 5'-methylthioinosine | 0.01 | 0.018 | 0.56 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V66I/V73I/Y160F, 5'-methylthioinosine |
| purine nucleoside phosphorylase (PfPNP)variant V66I/V73I/Y160F | Plasmodium falciparum | inosine | 0.36 | 0.004 | 90 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V66I/V73I/Y160F, inosine |
| purine nucleoside phosphorylase (PfPNP)variant V66I/Y160F | Plasmodium falciparum | 5'-methylthioinosine | 0.05 | 0.011 | 4.7 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V66I/Y160F, 5'-methylthioinosine |
| purine nucleoside phosphorylase (PfPNP)variant V66I/Y160F | Plasmodium falciparum | inosine | 1.2 | 0.0062 | 190 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V66I/Y160F, inosine |
| purine nucleoside phosphorylase (PfPNP)variant V66S | Plasmodium falciparum | 5'-methylthioinosine | 0.21 | 7 × 10⁻⁴ | 280 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V66S, 5'-methylthioinosine |
| purine nucleoside phosphorylase (PfPNP)variant V66S | Plasmodium falciparum | inosine | 1.4 | 0.078 | 18 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V66S, inosine |
| purine nucleoside phosphorylase (PfPNP)variant V73A | Plasmodium falciparum | 5'-methylthioinosine | 1.9 | 0.02 | 97 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V73A, 5'-methylthioinosine |
| purine nucleoside phosphorylase (PfPNP)variant V73A | Plasmodium falciparum | inosine | 1.1 | 0.007 | 160 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V73A, inosine |
| purine nucleoside phosphorylase (PfPNP)variant V73I | Plasmodium falciparum | 5'-methylthioinosine | 0.81 | 0.011 | 71 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V73I, 5'-methylthioinosine |
| purine nucleoside phosphorylase (PfPNP)variant V73I | Plasmodium falciparum | inosine | 1.9 | 0.013 | 140 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V73I, inosine |
| purine nucleoside phosphorylase (PfPNP)variant V73I/Y160F | Plasmodium falciparum | 5'-methylthioinosine | 0.28 | 0.06 | 4.6 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V73I/Y160F, 5'-methylthioinosine |
| purine nucleoside phosphorylase (PfPNP)variant V73I/Y160F | Plasmodium falciparum | inosine | 0.59 | 0.0036 | 160 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V73I/Y160F, inosine |
| purine nucleoside phosphorylase (PfPNP)variant V73S | Plasmodium falciparum | 5'-methylthioinosine | 0.02 | 2.1 | 0.011 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V73S, 5'-methylthioinosine |
| purine nucleoside phosphorylase (PfPNP)variant V73S | Plasmodium falciparum | inosine | 0.2 | 0.005 | 46 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant V73S, inosine |
| purine nucleoside phosphorylase (PfPNP)variant Y160A | Plasmodium falciparum | 5'-methylthioinosine | 0.09 | 0.2 | 0.45 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant Y160A, 5'-methylthioinosine |
| purine nucleoside phosphorylase (PfPNP)variant Y160A | Plasmodium falciparum | inosine | 0.1 | 5.4 | 0.22 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant Y160A, inosine |
| purine nucleoside phosphorylase (PfPNP)variant Y160F | Plasmodium falciparum | 5'-methylthioinosine | 0.3 | 0.044 | 6.8 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant Y160F, 5'-methylthioinosine |
| purine nucleoside phosphorylase (PfPNP)variant Y160F | Plasmodium falciparum | inosine | 0.21 | 0.0025 | 83 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant Y160F, inosine |
| purine nucleoside phosphorylase (PfPNP)variant Y47A | Plasmodium falciparum | 5'-methylthioinosine | 0.02 | 0.96 | 0.021 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant Y47A, 5'-methylthioinosine |
| purine nucleoside phosphorylase (PfPNP)variant Y47A | Plasmodium falciparum | inosine | 0.04 | 0.2 | 0.19 | pH 7.4 | Open record: purine nucleoside phosphorylase (PfPNP) variant Y47A, inosine |