CatLogenzyme kinetics catalog

Values CatLog read from this paper

Purine nucleoside phosphorylase from Escherichia coli and Salmonella typhimurium. Purification and some properties

PMID 235429doi:10.1111/j.1432-1033.1975.tb03925.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 15 records (15 values) from this paper: purine nucleoside phosphorylase from Escherichia coli, Salmonella typhimurium.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.4.2.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
purine nucleoside phosphorylaseEscherichia coliadenineassay procedure C: nucleoside synthesis; ribose 1-P (1.0 mM)—0.04—pH 7.1, 37 °COpen
purine nucleoside phosphorylaseEscherichia coliadenineassay procedure C: nucleoside synthesis—0.04—pH 7.1, 37 °COpen
purine nucleoside phosphorylaseEscherichia coliadenineassay procedure D: ribosyl transfer—0.11—pH 7.1, 37 °COpen
purine nucleoside phosphorylaseEscherichia colideoxyinosine—0.18—pH 7.1, 37 °COpen
purine nucleoside phosphorylaseEscherichia colideoxyribose 1-phosphate—0.1—pH 7.1, 37 °COpen
purine nucleoside phosphorylaseEscherichia coliinosinepotassium phosphate buffer pH 7.1; phosphate (180 mM)—0.07—pH 7.1, 37 °COpen
purine nucleoside phosphorylaseEscherichia coliinosineassay procedure D: ribosyl transfer—0.55—pH 7.1, 37 °COpen
purine nucleoside phosphorylaseEscherichia coliribose 1-phosphate—0.04—pH 7.1, 37 °COpen
purine nucleoside phosphorylaseSalmonella typhimuriumadenine—0.11—pH 7.1, 37 °COpen
purine nucleoside phosphorylaseSalmonella typhimuriumdeoxyadenosine—0.43—pH 7.1, 37 °COpen
purine nucleoside phosphorylaseSalmonella typhimuriumdeoxyguanosine—0.14—pH 7.1, 37 °COpen
purine nucleoside phosphorylaseSalmonella typhimuriumdeoxyinosinepotassium phosphate buffer pH 7.1—0.17—pH 7.1, 37 °COpen
purine nucleoside phosphorylaseSalmonella typhimuriumdeoxyinosineassay procedure B: arsenolysis of deoxyribonucleoside—0.21—pH 7.1, 37 °COpen
purine nucleoside phosphorylaseSalmonella typhimuriumdeoxyribose 1-phosphate—0.1—pH 7.1, 37 °COpen
purine nucleoside phosphorylaseSalmonella typhimuriuminosine—0.09—pH 7.1, 37 °COpen