Values CatLog read from this paper
Purine nucleoside phosphorylase. 1. Structure-function studies
PMID 9305962doi:10.1021/bi961969wOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 45 records (126 values) from this paper: purine nucleoside phosphorylase (human PNP, recombinant), purine nucleoside phosphorylase (human erythrocytic PNP), purine nucleoside phosphorylase from Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| purine nucleoside phosphorylasevariant N243A | Homo sapiens | phosphate | — | — | 0.03 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase variant N243A, phosphate |
| purine nucleoside phosphorylase (human erythrocytic PNP) | Homo sapiens | alpha-D-ribose 1-phosphate | — | 0.29 | — | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human erythrocytic PNP), alpha-D-ribose 1-phosphate |
| purine nucleoside phosphorylase (human erythrocytic PNP) | Homo sapiens | hypoxanthine | — | 0.012 | — | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human erythrocytic PNP), hypoxanthine |
| purine nucleoside phosphorylase (human erythrocytic PNP) | Homo sapiens | inosine | — | 0.029 | — | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human erythrocytic PNP), inosine |
| purine nucleoside phosphorylase (human PNP, recombinant) | Homo sapiens | hypoxanthine | 110 | 0.03 | 3.7 × 10³ | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant), hypoxanthine |
| purine nucleoside phosphorylase (human PNP, recombinant) | Homo sapiens | inosine | 56 | 0.04 | 1.4 × 10³ | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant), inosine |
| purine nucleoside phosphorylase (human PNP, recombinant) | Homo sapiens | phosphate | 56 | 4 | 14 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant), phosphate |
| purine nucleoside phosphorylase (human PNP, recombinant)variant E201A | Homo sapiens | hypoxanthine | 1.4 | 1 | 1 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant E201A, hypoxanthine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant E201A | Homo sapiens | inosine | 4.2 | 8.4 | 0.5 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant E201A, inosine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant E201A | Homo sapiens | phosphate | 1.8 | 6.1 | 0.1 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant E201A, phosphate |
| purine nucleoside phosphorylase (human PNP, recombinant)variant E89A | Homo sapiens | inosine | 46 | 0.22 | 210 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant E89A, inosine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant E89A | Homo sapiens | phosphate | 53 | 91 | 0.6 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant E89A, phosphate |
| purine nucleoside phosphorylase (human PNP, recombinant)variant F159A | Homo sapiens | hypoxanthine | 24 | 0.025 | 1 × 10³ | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant F159A, hypoxanthine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant F159A | Homo sapiens | inosine | 32 | 0.31 | 100 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant F159A, inosine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant F159A | Homo sapiens | phosphate | 30 | 2.5 | 10 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant F159A, phosphate |
| purine nucleoside phosphorylase (human PNP, recombinant)variant F200A | Homo sapiens | hypoxanthine | 14 | 0.57 | 24 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant F200A, hypoxanthine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant F200A | Homo sapiens | inosine | 9.4 | 19 | 0.5 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant F200A, inosine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant F200A | Homo sapiens | phosphate | 6.2 | 4.5 | 1 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant F200A, phosphate |
| purine nucleoside phosphorylase (human PNP, recombinant)variant H257A | Homo sapiens | hypoxanthine | 10 | 0.02 | 500 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant H257A, hypoxanthine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant H257A | Homo sapiens | inosine | 16 | 0.21 | 70 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant H257A, inosine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant H257A | Homo sapiens | phosphate | 13 | 2.5 | 5 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant H257A, phosphate |
| purine nucleoside phosphorylase (human PNP, recombinant)variant H86A | Homo sapiens | hypoxanthine | 0.97 | 0.019 | 50 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant H86A, hypoxanthine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant H86A | Homo sapiens | inosine | 14 | 0.28 | 50 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant H86A, inosine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant H86A | Homo sapiens | phosphate | 9.4 | 9.2 | 0.8 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant H86A, phosphate |
| purine nucleoside phosphorylase (human PNP, recombinant)variant K244A | Homo sapiens | hypoxanthine | 98 | 0.04 | 2.5 × 10³ | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant K244A, hypoxanthine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant K244A | Homo sapiens | inosine | 49 | 0.037 | 1.3 × 10³ | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant K244A, inosine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant K244A | Homo sapiens | phosphate | 52 | 2.7 | 19 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant K244A, phosphate |
| purine nucleoside phosphorylase (human PNP, recombinant)variant M219A | Homo sapiens | hypoxanthine | 130 | 0.18 | 720 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant M219A, hypoxanthine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant M219A | Homo sapiens | inosine | 52 | 8 | 10 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant M219A, inosine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant M219A | Homo sapiens | phosphate | 41 | 4.3 | 10 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant M219A, phosphate |
| purine nucleoside phosphorylase (human PNP, recombinant)variant N243A | Homo sapiens | hypoxanthine | 13 | 2.3 | 6 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant N243A, hypoxanthine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant N243A | Homo sapiens | inosine | 0.06 | 0.022 | 3 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant N243A, inosine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant N243A | Homo sapiens | phosphate | 0.09 | 3.5 | 0.026 calc. | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant N243A, phosphate |
| purine nucleoside phosphorylase (human PNP, recombinant)variant R84A | Homo sapiens | hypoxanthine | 0.5 | 0.02 | 25 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant R84A, hypoxanthine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant R84A | Homo sapiens | inosine | 2.1 | 0.13 | 16 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant R84A, inosine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant R84A | Homo sapiens | phosphate | 1.5 | 4.5 | 0.3 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant R84A, phosphate |
| purine nucleoside phosphorylase (human PNP, recombinant)variant S33A | Homo sapiens | hypoxanthine | 2.1 | 0.013 | 150 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant S33A, hypoxanthine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant S33A | Homo sapiens | inosine | 11 | 0.14 | 80 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant S33A, inosine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant S33A | Homo sapiens | phosphate | 11 | 3.6 | 3 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant S33A, phosphate |
| purine nucleoside phosphorylase (human PNP, recombinant)variant T242A | Homo sapiens | hypoxanthine | 58 | 0.024 | 2.4 × 10³ | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant T242A, hypoxanthine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant T242A | Homo sapiens | inosine | 14 | 0.022 | 640 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant T242A, inosine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant T242A | Homo sapiens | phosphate | 12 | 0.22 | 60 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant T242A, phosphate |
| purine nucleoside phosphorylase (human PNP, recombinant)variant Y88F | Homo sapiens | hypoxanthine | 9 | 0.01 | 900 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant Y88F, hypoxanthine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant Y88F | Homo sapiens | inosine | 54 | 0.058 | 930 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant Y88F, inosine |
| purine nucleoside phosphorylase (human PNP, recombinant)variant Y88F | Homo sapiens | phosphate | 56 | 2.1 | 30 | pH 7, 25 °C | Open record: purine nucleoside phosphorylase (human PNP, recombinant) variant Y88F, phosphate |