Values CatLog read from this paper
Amino acid discrimination by a highly differentiated metal center of an aminoacyl-tRNA synthetase
PMID 12974627doi:10.1021/bi034812uOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 41 records (110 values) from this paper: cysteinyl-tRNA synthetase (CysRS) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cysteinyl-tRNA synthetase (CysRS) | Escherichia coli | adenosine 5'-triphosphate | 141.9 | — | 560 | Open record: cysteinyl-tRNA synthetase (CysRS), adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS) | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 2.46 | — | 2.12 × 10³ | Open record: cysteinyl-tRNA synthetase (CysRS), E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS) | Escherichia coli | L-cysteine | 99.6 | — | 3.23 × 10³ | Open record: cysteinyl-tRNA synthetase (CysRS), L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant C209S | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.00413 | 0.0635 | 0.065 | Open record: cysteinyl-tRNA synthetase (CysRS) variant C209S, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant C28S | Escherichia coli | adenosine 5'-triphosphate | 0.35 | 1.95 | 0.179 | Open record: cysteinyl-tRNA synthetase (CysRS) variant C28S, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant C28S | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | — | 0.0221 | 0.255 | Open record: cysteinyl-tRNA synthetase (CysRS) variant C28S, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant C28S | Escherichia coli | L-cysteine | 0.13 | 2.36 | 0.0559 | Open record: cysteinyl-tRNA synthetase (CysRS) variant C28S, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant C28S | Escherichia coli | tRNACys | 0.00563 | — | — | Open record: cysteinyl-tRNA synthetase (CysRS) variant C28S, tRNACys | |
| cysteinyl-tRNA synthetase (CysRS)variant C28S/C209S | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.00527 | — | 0.0554 | Open record: cysteinyl-tRNA synthetase (CysRS) variant C28S/C209S, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant E238S | Escherichia coli | adenosine 5'-triphosphate | 34.4 | 0.98 | 35 | Open record: cysteinyl-tRNA synthetase (CysRS) variant E238S, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant E238S | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.59 | 8.3 × 10⁻⁴ | 710 | Open record: cysteinyl-tRNA synthetase (CysRS) variant E238S, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant E238S | Escherichia coli | L-cysteine | 30.4 | 0.05 | 608 | Open record: cysteinyl-tRNA synthetase (CysRS) variant E238S, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant H206S | Escherichia coli | adenosine 5'-triphosphate | 54.1 | 0.37 | 146 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H206S, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant H206S | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 1.08 | 7.8 × 10⁻⁴ | 1.38 × 10³ | Open record: cysteinyl-tRNA synthetase (CysRS) variant H206S, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant H206S | Escherichia coli | L-cysteine | 37.8 | 0.052 | 727 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H206S, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant H224N/H235N | Escherichia coli | adenosine 5'-triphosphate | 31.1 | 2.48 | 12.5 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H224N/H235N, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant H224N/H235N | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.021 | 0.0232 | 0.9 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H224N/H235N, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant H224N/H235N | Escherichia coli | L-cysteine | 8.98 | 7.09 | 1.27 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H224N/H235N, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant H224S | Escherichia coli | adenosine 5'-triphosphate | 58.8 | 1.41 | 41.7 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H224S, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant H224S | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.257 | 0.00214 | 120 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H224S, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant H224S | Escherichia coli | L-cysteine | 36.5 | 2.78 | 13.1 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H224S, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant H234N/E238Q | Escherichia coli | adenosine 5'-triphosphate | 0.0276 | 1.7 | 0.0162 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H234N/E238Q, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant H234N/E238Q | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.03 | 0.2397 | 0.125 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H234N/E238Q, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant H234N/E238Q | Escherichia coli | L-cysteine | 0.022 | 3.04 | 0.00724 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H234N/E238Q, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant H234S | Escherichia coli | adenosine 5'-triphosphate | 0.0032 | 0.4 | 0.00801 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H234S, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant H234S | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.018 | 0.0576 | 0.311 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H234S, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant H235S | Escherichia coli | adenosine 5'-triphosphate | 1.44 | 1.57 | 0.917 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H235S, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant H235S | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.039 | 0.00483 | 8.07 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H235S, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant H235S | Escherichia coli | L-cysteine | 1.32 | 2.58 | 0.512 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H235S, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant H256S | Escherichia coli | adenosine 5'-triphosphate | 1.38 | 0.42 | 3.28 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H256S, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant H256S | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.57 | 0.00267 | 213 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H256S, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant H256S | Escherichia coli | L-cysteine | 1.12 | 0.21 | 5.33 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H256S, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant W205A | Escherichia coli | adenosine 5'-triphosphate | 0.2 | 0.69 | 0.296 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205A, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant W205A | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.039 | 0.00489 | 8 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205A, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant W205A | Escherichia coli | L-cysteine | 0.22 | 6.58 | 0.0328 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205A, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant W205F | Escherichia coli | adenosine 5'-triphosphate | 0.55 | 1.16 | 0.481 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205F, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant W205F | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | — | — | 11.7 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205F, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant W205F | Escherichia coli | L-cysteine | 0.28 | 2.52 | 0.109 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205F, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant W205Y | Escherichia coli | adenosine 5'-triphosphate | 0.29 | — | 0.382 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205Y, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant W205Y | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.00674 | — | 1.02 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205Y, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant W205Y | Escherichia coli | L-cysteine | — | — | 0.0764 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205Y, L-cysteine |