EC 6.1.1.16: cysteine-tRNA ligase
LigasesOpen in Browse
CatLog read 65 records for EC 6.1.1.16 from 3 papers, for 1 organism. Names in these papers: cysteinyl-tRNA synthetase (CysRS).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 64 records for EC 6.1.1.16 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.1.1.16 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Amino acid discrimination by a highly differentiated metal center of an aminoacyl-tRNA synthetase | Escherichia coli | 41 |
| Zinc-mediated amino acid discrimination in cysteinyl-tRNA synthetase | Escherichia coli | 13 |
| Allosteric communication in cysteinyl tRNA synthetase: a network of direct and indirect readout | Escherichia coli | 11 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| cysteinyl-tRNA synthetase (CysRS) | Escherichia coli | adenosine 5'-triphosphate | 91 | 0.29 | 3.1 × 10³ | PMID 12662918 | Open record: cysteinyl-tRNA synthetase (CysRS), adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS) | Escherichia coli | adenosine 5'-triphosphateZn2+ enzyme (0.98 mol Zn2+/mol); C-terminal His-tag | 142 | 0.25 | 5.6 × 10³ | PMID 12662918 | Open record: cysteinyl-tRNA synthetase (CysRS), adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS) | Escherichia coli | adenosine 5'-triphosphateCo2+-substituted enzyme (0.92 mol Co2+/mol) | 57 | 0.22 | 2.6 × 10³ | PMID 12662918 | Open record: cysteinyl-tRNA synthetase (CysRS), adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS) | Escherichia coli | adenosine 5'-triphosphate | 141.9 | — | 560 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS), adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS) | Escherichia coli | cysteine | 79 | 0.022 | 3.6 × 10³ | PMID 12662918 | Open record: cysteinyl-tRNA synthetase (CysRS), cysteine | |
| cysteinyl-tRNA synthetase (CysRS) | Escherichia coli | cysteineZn2+ enzyme (0.98 mol Zn2+/mol); C-terminal His-tag | 100 | 0.031 | 3.2 × 10³ | PMID 12662918 | Open record: cysteinyl-tRNA synthetase (CysRS), cysteine | |
| cysteinyl-tRNA synthetase (CysRS) | Escherichia coli | cysteineCo2+-substituted enzyme (0.92 mol Co2+/mol) | 35 | 0.029 | 1.2 × 10³ | PMID 12662918 | Open record: cysteinyl-tRNA synthetase (CysRS), cysteine | |
| cysteinyl-tRNA synthetase (CysRS) | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 2.46 | — | 2.12 × 10³ | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS), E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS) | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 1.2 | 0.0012 | 1.1 × 10³ | PMID 21890630 | Open record: cysteinyl-tRNA synthetase (CysRS), E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS) | Escherichia coli | L-cysteine | 99.6 | — | 3.23 × 10³ | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS), L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS) | Escherichia coli | tRNA^{Cys} | 0.9 | 3.5 × 10⁻⁴ | 2.5 × 10³ | PMID 12662918 | Open record: cysteinyl-tRNA synthetase (CysRS), tRNA^{Cys} | |
| cysteinyl-tRNA synthetase (CysRS) | Escherichia coli | tRNA^{Cys}Zn2+ enzyme (0.98 mol Zn2+/mol); C-terminal His-tag | 2.5 | 0.00116 | 2.1 × 10³ | PMID 12662918 | Open record: cysteinyl-tRNA synthetase (CysRS), tRNA^{Cys} | |
| cysteinyl-tRNA synthetase (CysRS) | Escherichia coli | tRNA^{Cys}Co2+-substituted enzyme (0.92 mol Co2+/mol) | 0.5 | 4.2 × 10⁻⁴ | 1.3 × 10³ | PMID 12662918 | Open record: cysteinyl-tRNA synthetase (CysRS), tRNA^{Cys} | |
| cysteinyl-tRNA synthetase (CysRS)variant C209S | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.00413 | 0.0635 | 0.065 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant C209S, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant C28S | Escherichia coli | adenosine 5'-triphosphate | 0.35 | 1.95 | 0.179 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant C28S, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant C28S | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | — | 0.0221 | 0.255 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant C28S, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant C28S | Escherichia coli | L-cysteine | 0.13 | 2.36 | 0.0559 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant C28S, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant C28S | Escherichia coli | tRNACys | 0.00563 | — | — | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant C28S, tRNACys | |
| cysteinyl-tRNA synthetase (CysRS)variant C28S/C209S | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.00527 | — | 0.0554 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant C28S/C209S, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant C28S/C209S | Escherichia coli | tRNA^{Cys} | 0.005 | 0.0952 | 0.055 | PMID 12662918 | Open record: cysteinyl-tRNA synthetase (CysRS) variant C28S/C209S, tRNA^{Cys} | |
| cysteinyl-tRNA synthetase (CysRS)variant E238S | Escherichia coli | adenosine 5'-triphosphate | 34.4 | 0.98 | 35 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant E238S, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant E238S | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.59 | 8.3 × 10⁻⁴ | 710 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant E238S, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant E238S | Escherichia coli | L-cysteine | 30.4 | 0.05 | 608 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant E238S, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant E354Q | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 0.082 | 5.2 × 10⁻⁴ | 160 | PMID 21890630 | Open record: cysteinyl-tRNA synthetase (CysRS) variant E354Q, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant E354Q/R427A | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 0.49 | 0.01 | 50 | PMID 21890630 | Open record: cysteinyl-tRNA synthetase (CysRS) variant E354Q/R427A, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant H206S | Escherichia coli | adenosine 5'-triphosphate | 54.1 | 0.37 | 146 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H206S, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant H206S | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 1.08 | 7.8 × 10⁻⁴ | 1.38 × 10³ | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H206S, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant H206S | Escherichia coli | L-cysteine | 37.8 | 0.052 | 727 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H206S, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant H224N/H235N | Escherichia coli | adenosine 5'-triphosphate | 31.1 | 2.48 | 12.5 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H224N/H235N, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant H224N/H235N | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.021 | 0.0232 | 0.9 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H224N/H235N, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant H224N/H235N | Escherichia coli | L-cysteine | 8.98 | 7.09 | 1.27 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H224N/H235N, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant H224S | Escherichia coli | adenosine 5'-triphosphate | 58.8 | 1.41 | 41.7 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H224S, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant H224S | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.257 | 0.00214 | 120 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H224S, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant H224S | Escherichia coli | L-cysteine | 36.5 | 2.78 | 13.1 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H224S, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant H234N/E238Q | Escherichia coli | adenosine 5'-triphosphate | 0.0276 | 1.7 | 0.0162 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H234N/E238Q, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant H234N/E238Q | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.03 | 0.2397 | 0.125 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H234N/E238Q, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant H234N/E238Q | Escherichia coli | L-cysteine | 0.022 | 3.04 | 0.00724 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H234N/E238Q, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant H234S | Escherichia coli | adenosine 5'-triphosphate | 0.0032 | 0.4 | 0.00801 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H234S, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant H234S | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.018 | 0.0576 | 0.311 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H234S, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant H235S | Escherichia coli | adenosine 5'-triphosphate | 1.44 | 1.57 | 0.917 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H235S, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant H235S | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.039 | 0.00483 | 8.07 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H235S, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant H235S | Escherichia coli | L-cysteine | 1.32 | 2.58 | 0.512 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H235S, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant H256S | Escherichia coli | adenosine 5'-triphosphate | 1.38 | 0.42 | 3.28 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H256S, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant H256S | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.57 | 0.00267 | 213 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H256S, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant H256S | Escherichia coli | L-cysteine | 1.12 | 0.21 | 5.33 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H256S, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant H297A | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 0.16 | 5.5 × 10⁻⁴ | 320 | PMID 21890630 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H297A, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant H40A | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 0.0093 | 0.002 | 5.4 | PMID 21890630 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H40A, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant H40A/R42A | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 0.01 | 0.0024 | 5.3 | PMID 21890630 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H40A/R42A, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant M294A | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 0.14 | 4.7 × 10⁻⁴ | 300 | PMID 21890630 | Open record: cysteinyl-tRNA synthetase (CysRS) variant M294A, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant M294A/H297A | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 0.088 | 6.6 × 10⁻⁴ | 140 | PMID 21890630 | Open record: cysteinyl-tRNA synthetase (CysRS) variant M294A/H297A, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant M294A/R427A | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 0.02 | 0.0076 | 3 | PMID 21890630 | Open record: cysteinyl-tRNA synthetase (CysRS) variant M294A/R427A, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant R427A | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 0.027 | 0.0075 | 3.8 | PMID 21890630 | Open record: cysteinyl-tRNA synthetase (CysRS) variant R427A, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant R42A | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 1.1 | 0.0018 | 750 | PMID 21890630 | Open record: cysteinyl-tRNA synthetase (CysRS) variant R42A, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant W205A | Escherichia coli | adenosine 5'-triphosphate | 0.2 | 0.69 | 0.296 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205A, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant W205A | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.039 | 0.00489 | 8 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205A, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant W205A | Escherichia coli | L-cysteine | 0.22 | 6.58 | 0.0328 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205A, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant W205F | Escherichia coli | adenosine 5'-triphosphate | 0.55 | 1.16 | 0.481 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205F, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant W205F | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | — | — | 11.7 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205F, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant W205F | Escherichia coli | L-cysteine | 0.28 | 2.52 | 0.109 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205F, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant W205Y | Escherichia coli | adenosine 5'-triphosphate | 0.3 | 0.77 | 3.8 | PMID 12662918 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205Y, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant W205Y | Escherichia coli | adenosine 5'-triphosphate | 0.29 | — | 0.382 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205Y, adenosine 5'-triphosphate | |
| cysteinyl-tRNA synthetase (CysRS)variant W205Y | Escherichia coli | cysteine | 0.28 | 3.61 | 0.076 | PMID 12662918 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205Y, cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant W205Y | Escherichia coli | E. coli tRNA(Cys) (T7 transcript) | 0.00674 | — | 1.02 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205Y, E. coli tRNA(Cys) (T7 transcript) | |
| cysteinyl-tRNA synthetase (CysRS)variant W205Y | Escherichia coli | L-cysteine | — | — | 0.0764 | PMID 12974627 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205Y, L-cysteine | |
| cysteinyl-tRNA synthetase (CysRS)variant W205Y | Escherichia coli | tRNA^{Cys} | 0.007 | 0.00662 | 1 | PMID 12662918 | Open record: cysteinyl-tRNA synthetase (CysRS) variant W205Y, tRNA^{Cys} |