Values CatLog read from this paper
Allosteric communication in cysteinyl tRNA synthetase: a network of direct and indirect readout
PMID 21890630doi:10.1074/jbc.m111.246702Open in Browse
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CatLog read 11 records (33 values) from this paper: cysteinyl-tRNA synthetase (CysRS) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cysteinyl-tRNA synthetase (CysRS) | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 1.2 | 0.0012 | 1.1 × 10³ | Open record: cysteinyl-tRNA synthetase (CysRS), E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant E354Q | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 0.082 | 5.2 × 10⁻⁴ | 160 | Open record: cysteinyl-tRNA synthetase (CysRS) variant E354Q, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant E354Q/R427A | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 0.49 | 0.01 | 50 | Open record: cysteinyl-tRNA synthetase (CysRS) variant E354Q/R427A, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant H297A | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 0.16 | 5.5 × 10⁻⁴ | 320 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H297A, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant H40A | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 0.0093 | 0.002 | 5.4 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H40A, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant H40A/R42A | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 0.01 | 0.0024 | 5.3 | Open record: cysteinyl-tRNA synthetase (CysRS) variant H40A/R42A, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant M294A | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 0.14 | 4.7 × 10⁻⁴ | 300 | Open record: cysteinyl-tRNA synthetase (CysRS) variant M294A, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant M294A/H297A | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 0.088 | 6.6 × 10⁻⁴ | 140 | Open record: cysteinyl-tRNA synthetase (CysRS) variant M294A/H297A, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant M294A/R427A | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 0.02 | 0.0076 | 3 | Open record: cysteinyl-tRNA synthetase (CysRS) variant M294A/R427A, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant R427A | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 0.027 | 0.0075 | 3.8 | Open record: cysteinyl-tRNA synthetase (CysRS) variant R427A, E. coli tRNA(Cys), T7 in vitro transcript | |
| cysteinyl-tRNA synthetase (CysRS)variant R42A | Escherichia coli | E. coli tRNA(Cys), T7 in vitro transcript | 1.1 | 0.0018 | 750 | Open record: cysteinyl-tRNA synthetase (CysRS) variant R42A, E. coli tRNA(Cys), T7 in vitro transcript |