Values CatLog read from this paper
Cloning and overexpression of Lactobacillus helveticus D-lactate dehydrogenase gene in Escherichia coli
PMID 1396685doi:10.1111/j.1432-1033.1992.tb17250.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (25 values) from this paper: D-lactate dehydrogenase from Lactobacillus helveticus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| D-lactate dehydrogenase | Lactobacillus helveticus | 2-oxobutyrate | 126 | 30 | 4.2 | pH 7.5, 25 °C | Open record: D-lactate dehydrogenase, 2-oxobutyrate |
| D-lactate dehydrogenase | Lactobacillus helveticus | bromopyruvate | 830 | 13 | 64 | pH 7.5, 25 °C | Open record: D-lactate dehydrogenase, bromopyruvate |
| D-lactate dehydrogenase | Lactobacillus helveticus | D-lactate | 100 | 66 | 1.5 | pH 7.5, 25 °C | Open record: D-lactate dehydrogenase, D-lactate |
| D-lactate dehydrogenase | Lactobacillus helveticus | glyoxylate | 351 | 67 | 5.2 | pH 7.5, 25 °C | Open record: D-lactate dehydrogenase, glyoxylate |
| D-lactate dehydrogenase | Lactobacillus helveticus | NADH | 100 | 0.01 | 1 × 10⁴ | pH 7.5, 25 °C | Open record: D-lactate dehydrogenase, NADH |
| D-lactate dehydrogenase | Lactobacillus helveticus | oxalacetatenative enzyme purified from L. helveticus ATCC 15009 | 295 | 3.6 | 82 | pH 7.5, 25 °C | Open record: D-lactate dehydrogenase, oxalacetate |
| D-lactate dehydrogenase | Lactobacillus helveticus | oxalacetaterecombinant; purified | 250 | 4.2 | 60 calc. | pH 7.5, 25 °C | Open record: D-lactate dehydrogenase, oxalacetate |
| D-lactate dehydrogenase | Lactobacillus helveticus | pyruvatenative enzyme purified from L. helveticus ATCC 15009 | 1392 | 1.25 | 1.1 × 10³ | pH 7.5, 25 °C | Open record: D-lactate dehydrogenase, pyruvate |
| D-lactate dehydrogenase | Lactobacillus helveticus | pyruvaterecombinant; purified | 1.33 × 10³ | 1.5 | 890 calc. | pH 7.5, 25 °C | Open record: D-lactate dehydrogenase, pyruvate |