Values CatLog read from this paper
Temperature adaptation of proteins: engineering mesophilic-like activity and stability in a cold-adapted alpha-amylase
PMID 14499602doi:10.1016/j.jmb.2003.07.014Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (16 values) from this paper: α-amylase (AHA), pig pancreatic α-amylase (PPA) from Pseudoalteromonas haloplanktis, Sus scrofa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| pig pancreatic α-amylase (PPA) | Sus scrofa | 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene | 291 | 0.065 | 4.48 × 10³ | pH 7.2, 25 °C | Open record: pig pancreatic α-amylase (PPA), 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene |
| pig pancreatic α-amylase (PPA) | Sus scrofa | 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene | 141 | — | — | pH 7.2, 15 °C | Open record: pig pancreatic α-amylase (PPA), 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene |
| α-amylase (AHA) | Pseudoalteromonas haloplanktis | 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene | 697 | 0.234 | 2.98 × 10³ | pH 7.2, 25 °C | Open record: α-amylase (AHA), 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene |
| α-amylase (AHA) | Pseudoalteromonas haloplanktis | 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene | 392 | — | — | pH 7.2, 15 °C | Open record: α-amylase (AHA), 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene |
| α-amylase (AHA)variant K300R/N150D/V196F/Q164I/T232V | Pseudoalteromonas haloplanktis | 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene | 308 | 0.066 | 4.67 × 10³ | pH 7.2, 25 °C | Open record: α-amylase (AHA) variant K300R/N150D/V196F/Q164I/T232V, 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene |
| α-amylase (AHA)variant K300R/N150D/V196F/Q164I/T232V | Pseudoalteromonas haloplanktis | 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene | 144 | — | — | pH 7.2, 15 °C | Open record: α-amylase (AHA) variant K300R/N150D/V196F/Q164I/T232V, 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene |
| α-amylase (AHA)variant Q58C/A99C/N150D/Q164I/V196F/T232V/K300R | Pseudoalteromonas haloplanktis | 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene | 331 | 0.086 | 3.87 × 10³ | pH 7.2, 25 °C | Open record: α-amylase (AHA) variant Q58C/A99C/N150D/Q164I/V196F/T232V/K300R, 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene |
| α-amylase (AHA)variant Q58C/A99C/N150D/Q164I/V196F/T232V/K300R | Pseudoalteromonas haloplanktis | 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene | 139 | — | — | pH 7.2, 15 °C | Open record: α-amylase (AHA) variant Q58C/A99C/N150D/Q164I/V196F/T232V/K300R, 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene |