EC 3.2.1.1: alpha-amylase
HydrolasesOpen in Browse
CatLog read 125 records for EC 3.2.1.1 from 9 papers, for 11 organisms. Names in these papers: α-amylase (AMA), alpha amylase (BLA), barley α-amylase isozyme 1 (AMY1), α-amylase and 7 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 98 records for EC 3.2.1.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.1.1 in Browse.
Papers
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| alpha amylase (BLA) | Bacillus licheniformis | 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside | 8.73 | 0.0121 | 770 | pH 6, 25 °C | PMID 18626642 | Open record: alpha amylase (BLA), 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside |
| alpha amylase (BLA) | Bacillus licheniformis | 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside | 34 | 0.281 | 140 | pH 4.5, 37 °C | PMID 18626642 | Open record: alpha amylase (BLA), 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside |
| alpha amylase (BLA) | Bacillus licheniformis | 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside | 175 | 0.201 | 870 | pH 5.5, 37 °C | PMID 18626642 | Open record: alpha amylase (BLA), 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside |
| alpha amylase (BLA) | Bacillus licheniformis | 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside | 205 | 0.147 | 1.42 × 10³ | pH 6.5, 37 °C | PMID 18626642 | Open record: alpha amylase (BLA), 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside |
| alpha amylase (BLA) | Bacillus licheniformis | starch | 1.31 × 10³ | — | — | pH 6, 25 °C | PMID 18626642 | Open record: alpha amylase (BLA), starch |
| alpha amylase (BLA)variant L134R | Bacillus licheniformis | 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside | 8.14 | 0.0117 | 710 | pH 6, 25 °C | PMID 18626642 | Open record: alpha amylase (BLA) variant L134R, 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside |
| alpha amylase (BLA)variant L134R | Bacillus licheniformis | 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside | 234 | 0.171 | 1.35 × 10³ | pH 4.5, 37 °C | PMID 18626642 | Open record: alpha amylase (BLA) variant L134R, 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside |
| alpha amylase (BLA)variant L134R | Bacillus licheniformis | 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside | 201 | 0.193 | 1.07 × 10³ | pH 5.5, 37 °C | PMID 18626642 | Open record: alpha amylase (BLA) variant L134R, 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside |
| alpha amylase (BLA)variant L134R | Bacillus licheniformis | 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside | 186 | 0.201 | 920 | pH 6.5, 37 °C | PMID 18626642 | Open record: alpha amylase (BLA) variant L134R, 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside |
| alpha amylase (BLA)variant L134R | Bacillus licheniformis | starch | 1.13 × 10³ | — | — | pH 6, 25 °C | PMID 18626642 | Open record: alpha amylase (BLA) variant L134R, starch |
| alpha amylase (BLA)variant L134R/S320A | Bacillus licheniformis | 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside | 7.86 | 0.0107 | 680 | pH 6, 25 °C | PMID 18626642 | Open record: alpha amylase (BLA) variant L134R/S320A, 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside |
| alpha amylase (BLA)variant L134R/S320A | Bacillus licheniformis | 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside | 266 | 0.137 | 1.91 × 10³ | pH 4.5, 37 °C | PMID 18626642 | Open record: alpha amylase (BLA) variant L134R/S320A, 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside |
| alpha amylase (BLA)variant L134R/S320A | Bacillus licheniformis | 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside | 223 | 0.185 | 1.23 × 10³ | pH 5.5, 37 °C | PMID 18626642 | Open record: alpha amylase (BLA) variant L134R/S320A, 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside |
| alpha amylase (BLA)variant L134R/S320A | Bacillus licheniformis | 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside | 209 | 0.19 | 1.1 × 10³ | pH 6.5, 37 °C | PMID 18626642 | Open record: alpha amylase (BLA) variant L134R/S320A, 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside |
| alpha amylase (BLA)variant L134R/S320A | Bacillus licheniformis | starch | 950 | — | — | pH 6, 25 °C | PMID 18626642 | Open record: alpha amylase (BLA) variant L134R/S320A, starch |
| alpha amylase (BLA)variant S320A | Bacillus licheniformis | 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside | 8.32 | 0.0113 | 740 | pH 6, 25 °C | PMID 18626642 | Open record: alpha amylase (BLA) variant S320A, 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside |
| alpha amylase (BLA)variant S320A | Bacillus licheniformis | 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside | 219 | 0.186 | 1.21 × 10³ | pH 4.5, 37 °C | PMID 18626642 | Open record: alpha amylase (BLA) variant S320A, 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside |
| alpha amylase (BLA)variant S320A | Bacillus licheniformis | 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside | 187 | 0.2 | 950 | pH 5.5, 37 °C | PMID 18626642 | Open record: alpha amylase (BLA) variant S320A, 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside |
| alpha amylase (BLA)variant S320A | Bacillus licheniformis | 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside | 175 | 0.205 | 880 | pH 6.5, 37 °C | PMID 18626642 | Open record: alpha amylase (BLA) variant S320A, 4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside |
| alpha amylase (BLA)variant S320A | Bacillus licheniformis | starch | 1.27 × 10³ | — | — | pH 6, 25 °C | PMID 18626642 | Open record: alpha amylase (BLA) variant S320A, starch |
| alpha-amylase AmyPG2 | Photobacterium gaetbulicola | soluble starch50 mM phosphate-citric acid buffer; no NaCl | 114.6 | — | — | pH 8, 25 °C | PMID 41964703 | Open record: alpha-amylase AmyPG2, soluble starch |
| alpha-amylase AmyPG2 | Photobacterium gaetbulicola | soluble starch50 mM phosphate-citric acid buffer with 2.0 M NaCl | 1248 | — | — | pH 8, 25 °C | PMID 41964703 | Open record: alpha-amylase AmyPG2, soluble starch |
| alpha-amylase AmyPG2variant I236A | Photobacterium gaetbulicola | soluble starch50 mM phosphate-citric acid buffer; no NaCl | 173.1 | — | — | pH 8, 25 °C | PMID 41964703 | Open record: alpha-amylase AmyPG2 variant I236A, soluble starch |
| alpha-amylase AmyPG2variant I236A | Photobacterium gaetbulicola | soluble starch50 mM phosphate-citric acid buffer with 2.0 M NaCl | 1913 | — | — | pH 8, 25 °C | PMID 41964703 | Open record: alpha-amylase AmyPG2 variant I236A, soluble starch |
| alpha-amylase AmyPG2variant I236V | Photobacterium gaetbulicola | soluble starch50 mM phosphate-citric acid buffer; no NaCl | 188.8 | — | — | pH 8, 25 °C | PMID 41964703 | Open record: alpha-amylase AmyPG2 variant I236V, soluble starch |
| alpha-amylase AmyPG2variant I236V | Photobacterium gaetbulicola | soluble starch50 mM phosphate-citric acid buffer with 2.0 M NaCl | 1899 | — | — | pH 8, 25 °C | PMID 41964703 | Open record: alpha-amylase AmyPG2 variant I236V, soluble starch |
| alpha-amylase AmyPG2variant S263A | Photobacterium gaetbulicola | soluble starch50 mM phosphate-citric acid buffer; no NaCl | 161.7 | — | — | pH 8, 25 °C | PMID 41964703 | Open record: alpha-amylase AmyPG2 variant S263A, soluble starch |
| alpha-amylase AmyPG2variant S263A | Photobacterium gaetbulicola | soluble starch50 mM phosphate-citric acid buffer with 2.0 M NaCl | 1402 | — | — | pH 8, 25 °C | PMID 41964703 | Open record: alpha-amylase AmyPG2 variant S263A, soluble starch |
| AmyB (intracellular alpha-amylase) | Thermotoga neapolitana | maltoheptaose | 25 | 3.37 | 7.5 | pH 6.5, 75 °C | PMID 19834705 | Open record: AmyB (intracellular alpha-amylase), maltoheptaose |
| AmyB (intracellular alpha-amylase) | Thermotoga neapolitana | maltopentaose | 283 | 1.69 | 167.5 | pH 6.5, 75 °C | PMID 19834705 | Open record: AmyB (intracellular alpha-amylase), maltopentaose |
| AmyB (intracellular alpha-amylase) | Thermotoga neapolitana | maltotriose | 809 | 1.26 | 642.1 | pH 6.5, 75 °C | PMID 19834705 | Open record: AmyB (intracellular alpha-amylase), maltotriose |
| AmyB (intracellular alpha-amylase) | Thermotoga neapolitana | p-nitrophenyl alpha-D-maltohexaoside | 34 | 2.46 | 13.9 | pH 6.5, 75 °C | PMID 19834705 | Open record: AmyB (intracellular alpha-amylase), p-nitrophenyl alpha-D-maltohexaoside |
| AmyB (intracellular alpha-amylase) | Thermotoga neapolitana | soluble starch | 0.02 | 0.07 | 0.286 | pH 6.5, 75 °C | PMID 19834705 | Open record: AmyB (intracellular alpha-amylase), soluble starch |
| barley alpha-amylase 1 (AMY1) | Hordeum vulgare (barley) | 2-chloro-4-nitrophenyl beta-D-maltoheptaoside | 36 | 0.7 | 48 | pH 6.8, 30 °C | PMID 18588886 | Open record: barley alpha-amylase 1 (AMY1), 2-chloro-4-nitrophenyl beta-D-maltoheptaoside |
| barley alpha-amylase 1 (AMY1) | Hordeum vulgare (barley) | amylose (potato type III, DP440) | 169 | — | — | pH 5.5 | PMID 18588886 | Open record: barley alpha-amylase 1 (AMY1), amylose (potato type III, DP440) |
| barley alpha-amylase 1 (AMY1)variant H395A | Hordeum vulgare (barley) | 2-chloro-4-nitrophenyl beta-D-maltoheptaoside | 21 | 0.91 | 23 | pH 6.8, 30 °C | PMID 18588886 | Open record: barley alpha-amylase 1 (AMY1) variant H395A, 2-chloro-4-nitrophenyl beta-D-maltoheptaoside |
| barley alpha-amylase 1 (AMY1)variant H395A | Hordeum vulgare (barley) | amylose (potato type III, DP440) | 75 | — | — | pH 5.5 | PMID 18588886 | Open record: barley alpha-amylase 1 (AMY1) variant H395A, amylose (potato type III, DP440) |
| barley alpha-amylase 1 (AMY1)variant Y105A/Y380A | Hordeum vulgare (barley) | amylose (potato type III, DP440) | 61 | — | — | pH 5.5 | PMID 18588886 | Open record: barley alpha-amylase 1 (AMY1) variant Y105A/Y380A, amylose (potato type III, DP440) |
| barley alpha-amylase 1 (AMY1)variant Y105A/Y380M | Hordeum vulgare (barley) | amylose (potato type III, DP440) | 56 | — | — | pH 5.5 | PMID 18588886 | Open record: barley alpha-amylase 1 (AMY1) variant Y105A/Y380M, amylose (potato type III, DP440) |
| barley alpha-amylase 1 (AMY1)variant Y380A/H395A | Hordeum vulgare (barley) | 2-chloro-4-nitrophenyl beta-D-maltoheptaoside | 35 | 1.4 | 25 | pH 6.8, 30 °C | PMID 18588886 | Open record: barley alpha-amylase 1 (AMY1) variant Y380A/H395A, 2-chloro-4-nitrophenyl beta-D-maltoheptaoside |
| barley alpha-amylase 1 (AMY1)variant Y380A/H395A | Hordeum vulgare (barley) | amylose (potato type III, DP440) | 163 | — | — | pH 5.5 | PMID 18588886 | Open record: barley alpha-amylase 1 (AMY1) variant Y380A/H395A, amylose (potato type III, DP440) |
| barley α-amylase isozyme 1 (AMY1) | Hordeum vulgare | 2-chloro-4-nitrophenyl β-D-maltoheptaoside | 119 | 1.7 | 70 | pH 6.8, 30 °C | PMID 14660599 | Open record: barley α-amylase isozyme 1 (AMY1), 2-chloro-4-nitrophenyl β-D-maltoheptaoside |
| barley α-amylase isozyme 1 (AMY1) | Hordeum vulgare | amylose DP17 | 165 | — | — | pH 5.5, 37 °C | PMID 14660599 | Open record: barley α-amylase isozyme 1 (AMY1), amylose DP17 |
| barley α-amylase isozyme 1 (AMY1)variant T212P | Hordeum vulgare | 2-chloro-4-nitrophenyl β-D-maltoheptaoside | 140 | 1.4 | 100 | pH 6.8, 30 °C | PMID 14660599 | Open record: barley α-amylase isozyme 1 (AMY1) variant T212P, 2-chloro-4-nitrophenyl β-D-maltoheptaoside |
| barley α-amylase isozyme 1 (AMY1)variant T212P | Hordeum vulgare | amylose DP17 | 264 | — | — | pH 5.5, 37 °C | PMID 14660599 | Open record: barley α-amylase isozyme 1 (AMY1) variant T212P, amylose DP17 |
| barley α-amylase isozyme 1 (AMY1)variant T212W | Hordeum vulgare | 2-chloro-4-nitrophenyl β-D-maltoheptaoside | 61 | 0.6 | 102 | pH 6.8, 30 °C | PMID 14660599 | Open record: barley α-amylase isozyme 1 (AMY1) variant T212W, 2-chloro-4-nitrophenyl β-D-maltoheptaoside |
| barley α-amylase isozyme 1 (AMY1)variant T212W | Hordeum vulgare | amylose DP17 | 154 | — | — | pH 5.5, 37 °C | PMID 14660599 | Open record: barley α-amylase isozyme 1 (AMY1) variant T212W, amylose DP17 |
| barley α-amylase isozyme 1 (AMY1)variant T212Y | Hordeum vulgare | 2-chloro-4-nitrophenyl β-D-maltoheptaoside | 127 | 2 | 64 | pH 6.8, 30 °C | PMID 14660599 | Open record: barley α-amylase isozyme 1 (AMY1) variant T212Y, 2-chloro-4-nitrophenyl β-D-maltoheptaoside |
| barley α-amylase isozyme 1 (AMY1)variant T212Y | Hordeum vulgare | amylose DP17 | 127 | — | — | pH 5.5, 37 °C | PMID 14660599 | Open record: barley α-amylase isozyme 1 (AMY1) variant T212Y, amylose DP17 |
| barley α-amylase isozyme 1 (AMY1)variant Y105A | Hordeum vulgare | amylose DP17 | 146 | — | — | pH 5.5, 37 °C | PMID 14660599 | Open record: barley α-amylase isozyme 1 (AMY1) variant Y105A, amylose DP17 |
| barley α-amylase isozyme 1 (AMY1)variant Y105A/T212W | Hordeum vulgare | 2-chloro-4-nitrophenyl β-D-maltoheptaoside | 24 | 3.1 | 8 | pH 6.8, 30 °C | PMID 14660599 | Open record: barley α-amylase isozyme 1 (AMY1) variant Y105A/T212W, 2-chloro-4-nitrophenyl β-D-maltoheptaoside |
| barley α-amylase isozyme 1 (AMY1)variant Y105A/T212W | Hordeum vulgare | amylose DP17 | 105 | — | — | pH 5.5, 37 °C | PMID 14660599 | Open record: barley α-amylase isozyme 1 (AMY1) variant Y105A/T212W, amylose DP17 |
| barley α-amylase isozyme 1 (AMY1)variant Y105A/T212Y | Hordeum vulgare | 2-chloro-4-nitrophenyl β-D-maltoheptaoside | 31 | 6 | 5 | pH 6.8, 30 °C | PMID 14660599 | Open record: barley α-amylase isozyme 1 (AMY1) variant Y105A/T212Y, 2-chloro-4-nitrophenyl β-D-maltoheptaoside |
| barley α-amylase isozyme 1 (AMY1)variant Y105A/T212Y | Hordeum vulgare | amylose DP17 | 78 | — | — | pH 5.5, 37 °C | PMID 14660599 | Open record: barley α-amylase isozyme 1 (AMY1) variant Y105A/T212Y, amylose DP17 |
| barley α-amylase isozyme 1 (AMY1)variant Y105F | Hordeum vulgare | 2-chloro-4-nitrophenyl β-D-maltoheptaoside | 203 | 4.9 | 41 | pH 6.8, 30 °C | PMID 14660599 | Open record: barley α-amylase isozyme 1 (AMY1) variant Y105F, 2-chloro-4-nitrophenyl β-D-maltoheptaoside |
| barley α-amylase isozyme 1 (AMY1)variant Y105F | Hordeum vulgare | amylose DP17 | 158 | — | — | pH 5.5, 37 °C | PMID 14660599 | Open record: barley α-amylase isozyme 1 (AMY1) variant Y105F, amylose DP17 |
| barley α-amylase isozyme 1 (AMY1)variant Y105W | Hordeum vulgare | 2-chloro-4-nitrophenyl β-D-maltoheptaoside | 132 | 1.3 | 102 | pH 6.8, 30 °C | PMID 14660599 | Open record: barley α-amylase isozyme 1 (AMY1) variant Y105W, 2-chloro-4-nitrophenyl β-D-maltoheptaoside |
| barley α-amylase isozyme 1 (AMY1)variant Y105W | Hordeum vulgare | amylose DP17 | 200 | — | — | pH 5.5, 37 °C | PMID 14660599 | Open record: barley α-amylase isozyme 1 (AMY1) variant Y105W, amylose DP17 |
| Pichia burtonii alpha-amylase (PBA) | Pichia burtonii | alpha-cyclodextrin | 0.399 | 14.9 | 0.0268 | pH 5.6, 40 °C | PMID 18071262 | Open record: Pichia burtonii alpha-amylase (PBA), alpha-cyclodextrin |
| Pichia burtonii alpha-amylase (PBA) | Pichia burtonii | beta-cyclodextrin | 31.1 | 12.3 | 2.53 | pH 5.6, 40 °C | PMID 18071262 | Open record: Pichia burtonii alpha-amylase (PBA), beta-cyclodextrin |
| Pichia burtonii alpha-amylase (PBA) | Pichia burtonii | gamma-cyclodextrin | 64.6 | 2.35 | 27.6 | pH 5.6, 40 °C | PMID 18071262 | Open record: Pichia burtonii alpha-amylase (PBA), gamma-cyclodextrin |
| Pichia burtonii alpha-amylase (PBA) | Pichia burtonii | maltohexaose | 44.2 | 0.71 | 62.2 | pH 5.6, 40 °C | PMID 18071262 | Open record: Pichia burtonii alpha-amylase (PBA), maltohexaose |
| Pichia burtonii alpha-amylase (PBA) | Pichia burtonii | maltopentaose | 37.2 | 0.55 | 67.8 | pH 5.6, 40 °C | PMID 18071262 | Open record: Pichia burtonii alpha-amylase (PBA), maltopentaose |
| Pichia burtonii alpha-amylase (PBA) | Pichia burtonii | maltose | 0.0258 | 6.4 | 0.00405 | pH 5.6, 40 °C | PMID 18071262 | Open record: Pichia burtonii alpha-amylase (PBA), maltose |
| Pichia burtonii alpha-amylase (PBA) | Pichia burtonii | maltotetraose | 5.87 | 0.48 | 12.3 | pH 5.6, 40 °C | PMID 18071262 | Open record: Pichia burtonii alpha-amylase (PBA), maltotetraose |
| Pichia burtonii alpha-amylase (PBA) | Pichia burtonii | maltotriose | 0.24 | 28.6 | 0.00826 | pH 5.6, 40 °C | PMID 18071262 | Open record: Pichia burtonii alpha-amylase (PBA), maltotriose |
| Pichia burtonii alpha-amylase (PBA) | Pichia burtonii | pullulan | 0.085 | — | — | pH 5.6, 40 °C | PMID 18071262 | Open record: Pichia burtonii alpha-amylase (PBA), pullulan |
| Pichia burtonii alpha-amylase (PBA) | Pichia burtonii | soluble starch | 91.4 | — | — | pH 5.6, 40 °C | PMID 18071262 | Open record: Pichia burtonii alpha-amylase (PBA), soluble starch |
| pig pancreatic α-amylase (PPA) | Sus scrofa | 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene | 291 | 0.065 | 4.48 × 10³ | pH 7.2, 25 °C | PMID 14499602 | Open record: pig pancreatic α-amylase (PPA), 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene |
| pig pancreatic α-amylase (PPA) | Sus scrofa | 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene | 141 | — | — | pH 7.2, 15 °C | PMID 14499602 | Open record: pig pancreatic α-amylase (PPA), 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene |
| Taka-amylase A (TAA) | Aspergillus oryzae | alpha-cyclodextrin | 0.24 | 9.39 | 0.0255 | pH 5.3, 25 °C | PMID 18071262 | Open record: Taka-amylase A (TAA), alpha-cyclodextrin |
| Taka-amylase A (TAA) | Aspergillus oryzae | beta-cyclodextrin | 1.31 | 9.13 | 0.144 | pH 5.3, 25 °C | PMID 18071262 | Open record: Taka-amylase A (TAA), beta-cyclodextrin |
| Taka-amylase A (TAA) | Aspergillus oryzae | gamma-cyclodextrin | 180 | 3.33 | 54 | pH 5.3, 25 °C | PMID 18071262 | Open record: Taka-amylase A (TAA), gamma-cyclodextrin |
| Taka-amylase A (TAA) | Aspergillus oryzae | maltohexaose | 277 | 6.77 | 40.9 | pH 5.3, 25 °C | PMID 18071262 | Open record: Taka-amylase A (TAA), maltohexaose |
| Taka-amylase A (TAA) | Aspergillus oryzae | maltopentaose | 30.4 | 4.5 | 6.75 | pH 5.3, 25 °C | PMID 18071262 | Open record: Taka-amylase A (TAA), maltopentaose |
| Taka-amylase A (TAA) | Aspergillus oryzae | maltose | 0.0181 | 129 | 1.5 × 10⁻⁴ | pH 5.3, 25 °C | PMID 18071262 | Open record: Taka-amylase A (TAA), maltose |
| Taka-amylase A (TAA) | Aspergillus oryzae | maltotetraose | 11.2 | 3.11 | 3.6 | pH 5.3, 25 °C | PMID 18071262 | Open record: Taka-amylase A (TAA), maltotetraose |
| Taka-amylase A (TAA) | Aspergillus oryzae | maltotriose | 2.51 | 45.7 | 0.0549 | pH 5.3, 25 °C | PMID 18071262 | Open record: Taka-amylase A (TAA), maltotriose |
| Taka-amylase A (TAA) | Aspergillus oryzae | pullulan | 11.3 | — | — | pH 5.3, 25 °C | PMID 18071262 | Open record: Taka-amylase A (TAA), pullulan |
| Taka-amylase A (TAA) | Aspergillus oryzae | soluble starch | 194 | — | — | pH 5.3, 25 °C | PMID 18071262 | Open record: Taka-amylase A (TAA), soluble starch |
| α-amylase | Candida antarctica | Amylopectin (potato) | 171 | — | — | pH 4.2, 40 °C | PMID 3106037 | Open record: α-amylase, Amylopectin (potato) |
| α-amylase | Candida antarctica | cyclomaltoheptaose | 29.4 | 13.6 | 2.16 calc. | pH 4.2, 40 °C | PMID 3106037 | Open record: α-amylase, cyclomaltoheptaose |
| α-amylase | Candida antarctica | cyclomaltohexaose | 10.7 | 52 | 0.21 calc. | pH 4.2, 40 °C | PMID 3106037 | Open record: α-amylase, cyclomaltohexaose |
| α-amylase | Candida antarctica | cyclomaltooctaose | 186 | 4.4 | 42 calc. | pH 4.2, 40 °C | PMID 3106037 | Open record: α-amylase, cyclomaltooctaose |
| α-amylase | Candida antarctica | Glycogen (oyster) | 166 | — | — | pH 4.2, 40 °C | PMID 3106037 | Open record: α-amylase, Glycogen (oyster) |
| α-amylase | Candida antarctica | Glycogen (rat liver) | 130 | — | — | pH 4.2, 40 °C | PMID 3106037 | Open record: α-amylase, Glycogen (rat liver) |
| α-amylase | Candida antarctica | Maltoheptaose | 105 | 2.7 | 39 calc. | pH 4.2, 40 °C | PMID 3106037 | Open record: α-amylase, Maltoheptaose |
| α-amylase | Candida antarctica | Maltopentaose | 94.7 | 3.1 | 31 calc. | pH 4.2, 40 °C | PMID 3106037 | Open record: α-amylase, Maltopentaose |
| α-amylase | Candida antarctica | Maltotriose | 1.6 | 6.5 | 0.25 calc. | pH 4.2, 40 °C | PMID 3106037 | Open record: α-amylase, Maltotriose |
| α-amylase | Candida antarctica | Short-chain amylose | 230 | 0.77 | 300 calc. | pH 4.2, 40 °C | PMID 3106037 | Open record: α-amylase, Short-chain amylose |
| α-amylase | Candida antarctica | Soluble starch (Merck) | 237 | — | — | pH 4.2, 40 °C | PMID 3106037 | Open record: α-amylase, Soluble starch (Merck) |
| α-amylase | Candida antarctica | Soluble starch (Zulkowsky type) | 206 | — | — | pH 4.2, 40 °C | PMID 3106037 | Open record: α-amylase, Soluble starch (Zulkowsky type) |
| α-amylase (AHA) | Pseudoalteromonas haloplanktis | 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene | 697 | 0.234 | 2.98 × 10³ | pH 7.2, 25 °C | PMID 14499602 | Open record: α-amylase (AHA), 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene |
| α-amylase (AHA) | Pseudoalteromonas haloplanktis | 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene | 392 | — | — | pH 7.2, 15 °C | PMID 14499602 | Open record: α-amylase (AHA), 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene |
| α-amylase (AHA)variant K300R/N150D/V196F/Q164I/T232V | Pseudoalteromonas haloplanktis | 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene | 308 | 0.066 | 4.67 × 10³ | pH 7.2, 25 °C | PMID 14499602 | Open record: α-amylase (AHA) variant K300R/N150D/V196F/Q164I/T232V, 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene |
| α-amylase (AHA)variant K300R/N150D/V196F/Q164I/T232V | Pseudoalteromonas haloplanktis | 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene | 144 | — | — | pH 7.2, 15 °C | PMID 14499602 | Open record: α-amylase (AHA) variant K300R/N150D/V196F/Q164I/T232V, 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene |
| α-amylase (AHA)variant Q58C/A99C/N150D/Q164I/V196F/T232V/K300R | Pseudoalteromonas haloplanktis | 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene | 331 | 0.086 | 3.87 × 10³ | pH 7.2, 25 °C | PMID 14499602 | Open record: α-amylase (AHA) variant Q58C/A99C/N150D/Q164I/V196F/T232V/K300R, 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene |
| α-amylase (AHA)variant Q58C/A99C/N150D/Q164I/V196F/T232V/K300R | Pseudoalteromonas haloplanktis | 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene | 139 | — | — | pH 7.2, 15 °C | PMID 14499602 | Open record: α-amylase (AHA) variant Q58C/A99C/N150D/Q164I/V196F/T232V/K300R, 4-nitrophenyl-α-D-maltoheptaoside-4,6-O-ethylidene |
| α-amylase (AMA) | Apis mellifera | 4-nitrophenyl α-D-glucopyranoside | — | — | 1.06 × 10⁻⁴ | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA), 4-nitrophenyl α-D-glucopyranoside |
| α-amylase (AMA) | Apis mellifera | 4-nitrophenyl α-maltoheptaoside | 333 | 0.0369 | 9.03 × 10³ | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA), 4-nitrophenyl α-maltoheptaoside |
| α-amylase (AMA) | Apis mellifera | 4-nitrophenyl α-maltohexaoside | 327 | 0.0393 | 8.31 × 10³ | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA), 4-nitrophenyl α-maltohexaoside |
| α-amylase (AMA) | Apis mellifera | 4-nitrophenyl α-maltoside | — | — | 0.108 | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA), 4-nitrophenyl α-maltoside |
| α-amylase (AMA) | Apis mellifera | 4-nitrophenyl α-maltotrioside | — | 2.25 | — | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA), 4-nitrophenyl α-maltotrioside |
| α-amylase (AMA) | Apis mellifera | amylose EX-1 (DP17) | 395 | — | — | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA), amylose EX-1 (DP17) |
| α-amylase (AMA) | Apis mellifera | p-nitrophenyl 4,6-benzylidene-α-maltoheptaoside | 104 | 0.00708 | 1.46 × 10⁴ | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA), p-nitrophenyl 4,6-benzylidene-α-maltoheptaoside |
| α-amylase (AMA) | Apis mellifera | p-nitrophenyl 4,6-benzylidene-α-maltohexaoside | 111 | 0.00576 | 1.93 × 10⁴ | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA), p-nitrophenyl 4,6-benzylidene-α-maltohexaoside |
| α-amylase (AMA) | Apis mellifera | p-nitrophenyl 4,6-benzylidene-α-maltopentaoside | 83.1 | 0.0256 | 3.25 × 10³ | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA), p-nitrophenyl 4,6-benzylidene-α-maltopentaoside |
| α-amylase (AMA) | Apis mellifera | p-nitrophenyl 4,6-benzylidene-α-maltoside | — | — | 0.317 | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA), p-nitrophenyl 4,6-benzylidene-α-maltoside |
| α-amylase (AMA) | Apis mellifera | p-nitrophenyl 4,6-benzylidene-α-maltotetraoside | 131 | 0.0943 | 1.39 × 10³ | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA), p-nitrophenyl 4,6-benzylidene-α-maltotetraoside |
| α-amylase (AMA) | Apis mellifera | p-nitrophenyl 4,6-benzylidene-α-maltotrioside | 10.1 | 1.05 | 9.67 | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA), p-nitrophenyl 4,6-benzylidene-α-maltotrioside |
| α-amylase (AMA)variant E251A | Apis mellifera | amylose EX-1 (DP17) | 301 | — | — | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA) variant E251A, amylose EX-1 (DP17) |
| α-amylase (AMA)variant E272A | Apis mellifera | amylose EX-1 (DP17) | 616 | — | — | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA) variant E272A, amylose EX-1 (DP17) |
| α-amylase (AMA)variant I246A | Apis mellifera | amylose EX-1 (DP17) | 290 | — | — | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA) variant I246A, amylose EX-1 (DP17) |
| α-amylase (AMA)variant K210A | Apis mellifera | amylose EX-1 (DP17) | 134 | — | — | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA) variant K210A, amylose EX-1 (DP17) |
| α-amylase (AMA)variant K268A | Apis mellifera | amylose EX-1 (DP17) | 421 | — | — | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA) variant K268A, amylose EX-1 (DP17) |
| α-amylase (AMA)variant L175A | Apis mellifera | amylose EX-1 (DP17) | 15.9 | — | — | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA) variant L175A, amylose EX-1 (DP17) |
| α-amylase (AMA)variant N281A | Apis mellifera | amylose EX-1 (DP17) | 367 | — | — | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA) variant N281A, amylose EX-1 (DP17) |
| α-amylase (AMA)variant N288A | Apis mellifera | amylose EX-1 (DP17) | 380 | — | — | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA) variant N288A, amylose EX-1 (DP17) |
| α-amylase (AMA)variant Q81A | Apis mellifera | amylose EX-1 (DP17) | 132 | — | — | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA) variant Q81A, amylose EX-1 (DP17) |
| α-amylase (AMA)variant V173A | Apis mellifera | amylose EX-1 (DP17) | 198 | — | — | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA) variant V173A, amylose EX-1 (DP17) |
| α-amylase (AMA)variant W285A | Apis mellifera | amylose EX-1 (DP17) | 350 | — | — | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA) variant W285A, amylose EX-1 (DP17) |
| α-amylase (AMA)variant W293A | Apis mellifera | amylose EX-1 (DP17) | 394 | — | — | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA) variant W293A, amylose EX-1 (DP17) |
| α-amylase (AMA)variant W77A | Apis mellifera | amylose EX-1 (DP17) | 163 | — | — | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA) variant W77A, amylose EX-1 (DP17) |
| α-amylase (AMA)variant Y161A | Apis mellifera | amylose EX-1 (DP17) | 420 | — | — | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA) variant Y161A, amylose EX-1 (DP17) |
| α-amylase (AMA)variant Y248A | Apis mellifera | amylose EX-1 (DP17) | 161 | — | — | pH 5, 37 °C | PMID 42588464 | Open record: α-amylase (AMA) variant Y248A, amylose EX-1 (DP17) |