Values CatLog read from this paper
Tyrosine 105 and threonine 212 at outermost substrate binding subsites -6 and +4 control substrate specificity, oligosaccharide cleavage patterns, and multiple binding modes of barley alpha-amylase…
PMID 14660599doi:10.1074/jbc.m312825200Open in Browse
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CatLog read 17 records (33 values) from this paper: barley α-amylase isozyme 1 (AMY1) from Hordeum vulgare.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| barley α-amylase isozyme 1 (AMY1) | Hordeum vulgare | 2-chloro-4-nitrophenyl β-D-maltoheptaoside | 119 | 1.7 | 70 | pH 6.8, 30 °C | Open record: barley α-amylase isozyme 1 (AMY1), 2-chloro-4-nitrophenyl β-D-maltoheptaoside |
| barley α-amylase isozyme 1 (AMY1) | Hordeum vulgare | amylose DP17 | 165 | — | — | pH 5.5, 37 °C | Open record: barley α-amylase isozyme 1 (AMY1), amylose DP17 |
| barley α-amylase isozyme 1 (AMY1)variant T212P | Hordeum vulgare | 2-chloro-4-nitrophenyl β-D-maltoheptaoside | 140 | 1.4 | 100 | pH 6.8, 30 °C | Open record: barley α-amylase isozyme 1 (AMY1) variant T212P, 2-chloro-4-nitrophenyl β-D-maltoheptaoside |
| barley α-amylase isozyme 1 (AMY1)variant T212P | Hordeum vulgare | amylose DP17 | 264 | — | — | pH 5.5, 37 °C | Open record: barley α-amylase isozyme 1 (AMY1) variant T212P, amylose DP17 |
| barley α-amylase isozyme 1 (AMY1)variant T212W | Hordeum vulgare | 2-chloro-4-nitrophenyl β-D-maltoheptaoside | 61 | 0.6 | 102 | pH 6.8, 30 °C | Open record: barley α-amylase isozyme 1 (AMY1) variant T212W, 2-chloro-4-nitrophenyl β-D-maltoheptaoside |
| barley α-amylase isozyme 1 (AMY1)variant T212W | Hordeum vulgare | amylose DP17 | 154 | — | — | pH 5.5, 37 °C | Open record: barley α-amylase isozyme 1 (AMY1) variant T212W, amylose DP17 |
| barley α-amylase isozyme 1 (AMY1)variant T212Y | Hordeum vulgare | 2-chloro-4-nitrophenyl β-D-maltoheptaoside | 127 | 2 | 64 | pH 6.8, 30 °C | Open record: barley α-amylase isozyme 1 (AMY1) variant T212Y, 2-chloro-4-nitrophenyl β-D-maltoheptaoside |
| barley α-amylase isozyme 1 (AMY1)variant T212Y | Hordeum vulgare | amylose DP17 | 127 | — | — | pH 5.5, 37 °C | Open record: barley α-amylase isozyme 1 (AMY1) variant T212Y, amylose DP17 |
| barley α-amylase isozyme 1 (AMY1)variant Y105A | Hordeum vulgare | amylose DP17 | 146 | — | — | pH 5.5, 37 °C | Open record: barley α-amylase isozyme 1 (AMY1) variant Y105A, amylose DP17 |
| barley α-amylase isozyme 1 (AMY1)variant Y105A/T212W | Hordeum vulgare | 2-chloro-4-nitrophenyl β-D-maltoheptaoside | 24 | 3.1 | 8 | pH 6.8, 30 °C | Open record: barley α-amylase isozyme 1 (AMY1) variant Y105A/T212W, 2-chloro-4-nitrophenyl β-D-maltoheptaoside |
| barley α-amylase isozyme 1 (AMY1)variant Y105A/T212W | Hordeum vulgare | amylose DP17 | 105 | — | — | pH 5.5, 37 °C | Open record: barley α-amylase isozyme 1 (AMY1) variant Y105A/T212W, amylose DP17 |
| barley α-amylase isozyme 1 (AMY1)variant Y105A/T212Y | Hordeum vulgare | 2-chloro-4-nitrophenyl β-D-maltoheptaoside | 31 | 6 | 5 | pH 6.8, 30 °C | Open record: barley α-amylase isozyme 1 (AMY1) variant Y105A/T212Y, 2-chloro-4-nitrophenyl β-D-maltoheptaoside |
| barley α-amylase isozyme 1 (AMY1)variant Y105A/T212Y | Hordeum vulgare | amylose DP17 | 78 | — | — | pH 5.5, 37 °C | Open record: barley α-amylase isozyme 1 (AMY1) variant Y105A/T212Y, amylose DP17 |
| barley α-amylase isozyme 1 (AMY1)variant Y105F | Hordeum vulgare | 2-chloro-4-nitrophenyl β-D-maltoheptaoside | 203 | 4.9 | 41 | pH 6.8, 30 °C | Open record: barley α-amylase isozyme 1 (AMY1) variant Y105F, 2-chloro-4-nitrophenyl β-D-maltoheptaoside |
| barley α-amylase isozyme 1 (AMY1)variant Y105F | Hordeum vulgare | amylose DP17 | 158 | — | — | pH 5.5, 37 °C | Open record: barley α-amylase isozyme 1 (AMY1) variant Y105F, amylose DP17 |
| barley α-amylase isozyme 1 (AMY1)variant Y105W | Hordeum vulgare | 2-chloro-4-nitrophenyl β-D-maltoheptaoside | 132 | 1.3 | 102 | pH 6.8, 30 °C | Open record: barley α-amylase isozyme 1 (AMY1) variant Y105W, 2-chloro-4-nitrophenyl β-D-maltoheptaoside |
| barley α-amylase isozyme 1 (AMY1)variant Y105W | Hordeum vulgare | amylose DP17 | 200 | — | — | pH 5.5, 37 °C | Open record: barley α-amylase isozyme 1 (AMY1) variant Y105W, amylose DP17 |