CatLogenzyme kinetics catalog

Values CatLog read from this paper

Acid stabilization of Bacillus licheniformis alpha amylase through introduction of mutations

PMID 18626642doi:10.1007/s00253-008-1580-5Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 20 records (52 values) from this paper: alpha amylase (BLA) from Bacillus licheniformis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.2.1.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
alpha amylase (BLA)Bacillus licheniformis4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside8.730.0121770pH 6, 25 °COpen
alpha amylase (BLA)Bacillus licheniformis4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside340.281140pH 4.5, 37 °COpen
alpha amylase (BLA)Bacillus licheniformis4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside1750.201870pH 5.5, 37 °COpen
alpha amylase (BLA)Bacillus licheniformis4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside2050.1471.42 × 10³pH 6.5, 37 °COpen
alpha amylase (BLA)Bacillus licheniformisstarch1.31 × 10³——pH 6, 25 °COpen
alpha amylase (BLA)variant L134RBacillus licheniformis4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside8.140.0117710pH 6, 25 °COpen
alpha amylase (BLA)variant L134RBacillus licheniformis4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside2340.1711.35 × 10³pH 4.5, 37 °COpen
alpha amylase (BLA)variant L134RBacillus licheniformis4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside2010.1931.07 × 10³pH 5.5, 37 °COpen
alpha amylase (BLA)variant L134RBacillus licheniformis4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside1860.201920pH 6.5, 37 °COpen
alpha amylase (BLA)variant L134RBacillus licheniformisstarch1.13 × 10³——pH 6, 25 °COpen
alpha amylase (BLA)variant L134R/S320ABacillus licheniformis4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside7.860.0107680pH 6, 25 °COpen
alpha amylase (BLA)variant L134R/S320ABacillus licheniformis4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside2660.1371.91 × 10³pH 4.5, 37 °COpen
alpha amylase (BLA)variant L134R/S320ABacillus licheniformis4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside2230.1851.23 × 10³pH 5.5, 37 °COpen
alpha amylase (BLA)variant L134R/S320ABacillus licheniformis4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside2090.191.1 × 10³pH 6.5, 37 °COpen
alpha amylase (BLA)variant L134R/S320ABacillus licheniformisstarch950——pH 6, 25 °COpen
alpha amylase (BLA)variant S320ABacillus licheniformis4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside8.320.0113740pH 6, 25 °COpen
alpha amylase (BLA)variant S320ABacillus licheniformis4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside2190.1861.21 × 10³pH 4.5, 37 °COpen
alpha amylase (BLA)variant S320ABacillus licheniformis4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside1870.2950pH 5.5, 37 °COpen
alpha amylase (BLA)variant S320ABacillus licheniformis4,6-ethylidene(G7)-p-nitrophenyl(G1)-alpha-D-maltoheptaoside1750.205880pH 6.5, 37 °COpen
alpha amylase (BLA)variant S320ABacillus licheniformisstarch1.27 × 10³——pH 6, 25 °COpen