Values CatLog read from this paper
Role of aspartate-133 and histidine-458 in the mechanism of tryptophan indole-lyase from Proteus vulgaris
PMID 14503866doi:10.1021/bi034348tOpen in Browse
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CatLog read 31 records (69 values) from this paper: tryptophan indole-lyase (tryptophanase, Trpase) from Proteus vulgaris.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| tryptophan indole-lyase (tryptophanase, Trpase) | Proteus vulgaris | H2-L-Trp | 1.66 | 0.18 | 9.32 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase), H2-L-Trp |
| tryptophan indole-lyase (tryptophanase, Trpase) | Proteus vulgaris | L-serine | 1.83 | 50.9 | 0.0359 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase), L-serine |
| tryptophan indole-lyase (tryptophanase, Trpase) | Proteus vulgaris | L-tryptophan | — | 0.28 | — | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase), L-tryptophan |
| tryptophan indole-lyase (tryptophanase, Trpase) | Proteus vulgaris | L-tryptophan | 6.29 | — | — | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase), L-tryptophan |
| tryptophan indole-lyase (tryptophanase, Trpase) | Proteus vulgaris | L-tryptophan | — | — | 22.5 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase), L-tryptophan |
| tryptophan indole-lyase (tryptophanase, Trpase) | Proteus vulgaris | L-tryptophanin D2O (solvent isotope effect) | — | 0.26 | — | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase), L-tryptophan |
| tryptophan indole-lyase (tryptophanase, Trpase) | Proteus vulgaris | L-tryptophanin D2O (solvent isotope effect) | 1.73 | — | — | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase), L-tryptophan |
| tryptophan indole-lyase (tryptophanase, Trpase) | Proteus vulgaris | L-tryptophanin D2O (solvent isotope effect) | — | — | 6.65 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase), L-tryptophan |
| tryptophan indole-lyase (tryptophanase, Trpase) | Proteus vulgaris | S-(o-nitrophenyl)-L-cysteine | 33.35 | 0.18 | 185 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase), S-(o-nitrophenyl)-L-cysteine |
| tryptophan indole-lyase (tryptophanase, Trpase) | Proteus vulgaris | S-benzyl-L-cysteine | 1.95 | 0.8 | 2.44 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase), S-benzyl-L-cysteine |
| tryptophan indole-lyase (tryptophanase, Trpase) | Proteus vulgaris | S-ethyl-L-cysteine | 3.59 | 3.17 | 1.13 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase), S-ethyl-L-cysteine |
| tryptophan indole-lyase (tryptophanase, Trpase) | Proteus vulgaris | S-methyl-L-cysteine | 4.1 | 16.8 | 0.238 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase), S-methyl-L-cysteine |
| tryptophan indole-lyase (tryptophanase, Trpase) | Proteus vulgaris | β-chloro-L-alanine | 8.41 | 9.36 | 0.898 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase), β-chloro-L-alanine |
| tryptophan indole-lyase (tryptophanase, Trpase)variant D133A | Proteus vulgaris | S-(o-nitrophenyl)-L-cysteine | 1.4 | 0.01 | 140 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase) variant D133A, S-(o-nitrophenyl)-L-cysteine |
| tryptophan indole-lyase (tryptophanase, Trpase)variant D133A | Proteus vulgaris | S-benzyl-L-cysteine | 0.87 | 0.01 | 87 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase) variant D133A, S-benzyl-L-cysteine |
| tryptophan indole-lyase (tryptophanase, Trpase)variant D133A | Proteus vulgaris | S-ethyl-L-cysteine | 0.21 | 0.03 | 7 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase) variant D133A, S-ethyl-L-cysteine |
| tryptophan indole-lyase (tryptophanase, Trpase)variant D133A | Proteus vulgaris | S-methyl-L-cysteine | 0.35 | 0.19 | 1.84 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase) variant D133A, S-methyl-L-cysteine |
| tryptophan indole-lyase (tryptophanase, Trpase)variant D133A | Proteus vulgaris | β-chloro-L-alanine | 10.8 | 4.6 | 2.35 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase) variant D133A, β-chloro-L-alanine |
| tryptophan indole-lyase (tryptophanase, Trpase)variant H458A | Proteus vulgaris | H2-L-Trp | 0.05 | 0.76 | 0.065 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase) variant H458A, H2-L-Trp |
| tryptophan indole-lyase (tryptophanase, Trpase)variant H458A | Proteus vulgaris | L-serine | 1.3 | 7.85 | 0.165 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase) variant H458A, L-serine |
| tryptophan indole-lyase (tryptophanase, Trpase)variant H458A | Proteus vulgaris | L-tryptophan | — | 1.15 | — | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase) variant H458A, L-tryptophan |
| tryptophan indole-lyase (tryptophanase, Trpase)variant H458A | Proteus vulgaris | L-tryptophan | 0.11 | — | — | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase) variant H458A, L-tryptophan |
| tryptophan indole-lyase (tryptophanase, Trpase)variant H458A | Proteus vulgaris | L-tryptophan | — | — | 0.095 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase) variant H458A, L-tryptophan |
| tryptophan indole-lyase (tryptophanase, Trpase)variant H458A | Proteus vulgaris | L-tryptophanin D2O (solvent isotope effect) | — | 1 | — | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase) variant H458A, L-tryptophan |
| tryptophan indole-lyase (tryptophanase, Trpase)variant H458A | Proteus vulgaris | L-tryptophanin D2O (solvent isotope effect) | 0.04 | — | — | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase) variant H458A, L-tryptophan |
| tryptophan indole-lyase (tryptophanase, Trpase)variant H458A | Proteus vulgaris | L-tryptophanin D2O (solvent isotope effect) | — | — | 0.04 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase) variant H458A, L-tryptophan |
| tryptophan indole-lyase (tryptophanase, Trpase)variant H458A | Proteus vulgaris | S-(o-nitrophenyl)-L-cysteine | 31.8 | 0.1 | 318 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase) variant H458A, S-(o-nitrophenyl)-L-cysteine |
| tryptophan indole-lyase (tryptophanase, Trpase)variant H458A | Proteus vulgaris | S-benzyl-L-cysteine | 10.9 | 0.096 | 113 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase) variant H458A, S-benzyl-L-cysteine |
| tryptophan indole-lyase (tryptophanase, Trpase)variant H458A | Proteus vulgaris | S-ethyl-L-cysteine | 6.41 | 0.42 | 15.2 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase) variant H458A, S-ethyl-L-cysteine |
| tryptophan indole-lyase (tryptophanase, Trpase)variant H458A | Proteus vulgaris | S-methyl-L-cysteine | 6.03 | 2.32 | 2.59 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase) variant H458A, S-methyl-L-cysteine |
| tryptophan indole-lyase (tryptophanase, Trpase)variant H458A | Proteus vulgaris | β-chloro-L-alanine | 23.3 | 5.6 | 4.16 | pH 8, 30 °C | Open record: tryptophan indole-lyase (tryptophanase, Trpase) variant H458A, β-chloro-L-alanine |