Values CatLog read from this paper
Allosteric and catalytic functions of the PPi-binding motif in the ATP sulfurylase-GTPase system
PMID 14506286doi:10.1074/jbc.m306897200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 18 records (32 values) from this paper: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 14 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) | Escherichia coli | adenosine 5'-phosphosulfate | 0.6 | 0.1 | 6.0 calc. | pH 8, 25 °C | Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit), adenosine 5'-phosphosulfate |
| ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) | Escherichia coli | guanosine 5'-triphosphateMgCl2 = [GTP] + 1.0 mM | 2.1 | 0.051 | 41 calc. | pH 8, 25 °C | Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit), guanosine 5'-triphosphate |
| ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) | Escherichia coli | guanosine 5'-triphosphateMgCl2 = [GTP] + [PPi] + 0.70 mM | 0.33 | 0.009 | 37 calc. | pH 8, 25 °C | Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit), guanosine 5'-triphosphate |
| ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) | Escherichia coli | inorganic pyrophosphate | — | 0.052 | — | pH 8, 25 °C | Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit), inorganic pyrophosphate |
| ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant D39A | Escherichia coli | guanosine 5'-triphosphateMgCl2 = [GTP] + 1.0 mM | 3.6 | 0.24 | 15 calc. | pH 8, 25 °C | Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant D39A, guanosine 5'-triphosphate |
| ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant D39A | Escherichia coli | guanosine 5'-triphosphateMgCl2 = [GTP] + [PPi] + 0.70 mM | 0.24 | 0.29 | 0.83 calc. | pH 8, 25 °C | Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant D39A, guanosine 5'-triphosphate |
| ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant G37A | Escherichia coli | adenosine 5'-phosphosulfate | 0.051 | 0.68 | 0.075 calc. | pH 8, 25 °C | Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant G37A, adenosine 5'-phosphosulfate |
| ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant G37A | Escherichia coli | guanosine 5'-triphosphateMgCl2 = [GTP] + 1.0 mM | 1.87 | 0.1 | 19 calc. | pH 8, 25 °C | Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant G37A, guanosine 5'-triphosphate |
| ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant G37A | Escherichia coli | guanosine 5'-triphosphateMgCl2 = [GTP] + [PPi] + 0.70 mM | 0.035 | 0.11 | 0.32 calc. | pH 8, 25 °C | Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant G37A, guanosine 5'-triphosphate |
| ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant G37A | Escherichia coli | inorganic pyrophosphate | — | 8.3 | — | pH 8, 25 °C | Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant G37A, inorganic pyrophosphate |
| ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant S35A | Escherichia coli | adenosine 5'-phosphosulfate | 0.85 | 0.13 | 6.5 calc. | pH 8, 25 °C | Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant S35A, adenosine 5'-phosphosulfate |
| ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant S35A | Escherichia coli | guanosine 5'-triphosphateMgCl2 = [GTP] + 1.0 mM | 1.5 | 0.05 | 30 calc. | pH 8, 25 °C | Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant S35A, guanosine 5'-triphosphate |
| ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant S35A | Escherichia coli | guanosine 5'-triphosphateMgCl2 = [GTP] + [PPi] + 0.70 mM | 0.24 | 0.085 | 2.8 calc. | pH 8, 25 °C | Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant S35A, guanosine 5'-triphosphate |
| ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant S35A | Escherichia coli | inorganic pyrophosphate | — | 0.16 | — | pH 8, 25 °C | Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant S35A, inorganic pyrophosphate |
| ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant S40A | Escherichia coli | adenosine 5'-phosphosulfate | 3.2 | 0.49 | 6.5 calc. | pH 8, 25 °C | Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant S40A, adenosine 5'-phosphosulfate |
| ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant S40A | Escherichia coli | guanosine 5'-triphosphateMgCl2 = [GTP] + 1.0 mM | 1 | 0.083 | 12 calc. | pH 8, 25 °C | Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant S40A, guanosine 5'-triphosphate |
| ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant S40A | Escherichia coli | guanosine 5'-triphosphateMgCl2 = [GTP] + [PPi] + 0.70 mM | 0.25 | 0.17 | 1.5 calc. | pH 8, 25 °C | Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant S40A, guanosine 5'-triphosphate |
| ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit)variant S40A | Escherichia coli | inorganic pyrophosphate | — | 1.8 | — | pH 8, 25 °C | Open record: ATP sulfurylase (CysD-CysN heterodimer; mutations in the CysD adenylyltransferase subunit) variant S40A, inorganic pyrophosphate |